Significant reduction in bacterial shedding and improvement in milk production in dairy farms after the use of a new inactivated paratuberculosis vaccine in a field trial

Juste, Ramón A.; Alonso‐Hearn, Marta; Molina, Elena; Geijó, Mariví; Vázquez, Patricia; Sevilla, Iker A.; Garrido, Joseba M.

doi:10.1186/1756-0500-2-233

Cited by 53 publications

(55 citation statements)

References 9 publications

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“…MAP DNA detection based on the amplification of the specific IS900 insertion sequence was carried out using the ADIAVET ® PARATB Real Time commercial kit (Adiagene, Saint Brieuc, France) and the ABI Prism 7000 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) as previously described (Juste et al, 2009). Samples were scored positive if their threshold cycle (Ct) was less than 40.00.…”

Section: Microbiological Findings: Map Isolation and Is900 Amplificationmentioning

confidence: 99%

Latent infections are the most frequent form of paratuberculosis in slaughtered Friesian cattle

Vázquez¹,

Garrido²,

Molina³

et al. 2014

Span. j. agric. res.

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Paratuberculosis is a chronic mycobacterial infection causing granulomatous enteritis in ruminants, whose pathogenesis and epidemiology poses numerous challenges, including latency and reactivation. The most recent and complete classification of paratuberculosis immunopathological types in cattle recognized five categories. In this study, 1031 slaughtered Friesian cattle were submitted to serological, microbiological and pathological examinations with the aim of maximizing the rate of case detection. In most cases, infected animals had minimal lesions and almost no other proof of infection (38.9%), while the more characteristic types with the whole constellation of microbiological and immunological evidences accounted for a lower proportion (7.7%). As these findings in cattle suggest similarities with the epidemiology of tuberculosis in humans, we propose to re-group the original immunopathological types into two broader paratuberculosis epidemio-pathogenic forms or states: latent and patent. The former term would define infections with focal lesions and might constitute an apparent resilience status representing a difficult to detect reservoir of infection whose role could become critical if later immune-compromising factors lead to re-activation. The latter would group those cases with multifocal and diffuse inflammatory lesions with higher mycobacterial load and viability suggestive of a more immediate epidemiological risk. Interestingly, the relative frequency of presentation of each profile varied with age. The proportion of latent forms remained relatively constant between 33.8% and 54.3% through adulthood from 3 years of age, while patent forms were more frequent during the first years of age and tended to decrease among the oldest individuals.

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Section: Microbiological Findings: Map Isolation and Is900 Amplificationmentioning

confidence: 99%

Latent infections are the most frequent form of paratuberculosis in slaughtered Friesian cattle

Vázquez¹,

Garrido²,

Molina³

et al. 2014

Span. j. agric. res.

Self Cite

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“…Briefly, 300 mL of the supernatant obtained after the cell disruption stage were transferred to Eppendorf tubes containing 20 mL of Lysis 2 buffer, firstly incubated at 70 ± 2 ºC for 10 min and next at 95 ± 2 °C for 15 min. The amplification of specific IS900 MAP DNA by using Adiavet® Map DNA extraction and detection kit (Adiagene, Saint Brieuc, France) and the ABI Prism 7000 Sequence Detection System (Applied Biosystems, Forter City, CA, USA) was carried out as previously described (Juste et al, 2009). Samples were scored as positive when reaching a threshold cycle (Ct) value of 40.00 or lower.…”

Section: Map Isolation From Tissues: Culture and Real Time Pcr (Rtpcr)mentioning

confidence: 99%

“…Then, an adapted protocol for tissue samples was performed using Adiapure® MAP DNA extraction and purification kit (Adiagene, Saint Brieuc, France). The protocol was carried out as described for fecal samples (Juste et al, 2009) but including an additional chemical lysis stage. Briefly, 300 mL of the supernatant obtained after the cell disruption stage were transferred to Eppendorf tubes containing 20 mL of Lysis 2 buffer, firstly incubated at 70 ± 2 ºC for 10 min and next at 95 ± 2 °C for 15 min.…”

Section: Map Isolation From Tissues: Culture and Real Time Pcr (Rtpcr)mentioning

confidence: 99%

Effects of paratuberculosis on Friesian cattle carcass weight and age at culling

Vázquez

Garrido

Juste

2012

Span. j. agric. res.

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Bovine paratuberculosis (PTB) causes major economic losses to dairy farmers because of decreased milk production, poor body condition, weight loss and early culling. The aim of this study was to evaluate the effect of Mycobacterium avium subspecies paratuberculosis (MAP) infection on carcass weight and age at slaughter in Friesian cattle. A total of 1,014 adult cows slaughtered at two local abattoirs in the Basque Country were included in this study. MAP infection was determined by different methods: indirect ELISA on blood samples, detection of MAP in tissues by culture and real time PCR (rtPCR), and histopathological examination. Serial and parallel interpretations of these methods were also considered for setting the upper and lower infection rates. MAP infection was confirmed by at least one test in 58.9% of animals. Most infected cows were detected by histopathology (46.9%) and rtPCR (29.6%). Overall estimates of carcass mean weight losses ranged from 3.7% for cases identified by the presence of microscopic specific PTB inflammatory lesions to 12.4% for cases with positive results in the paratuberculosis antibody ELISA test. Isolation of high bacterial loads in tissues and occurrence of diffuse granulomatous enteritis were associated with the highest weight loses, 22.2% and 26.0% respectively. The life expectancy of seropositive cows and those showing diffuse lesions was reduced by nearly one year compared to that of non-infected ones. Our results provide consistent evidences of PTB effect on the reduction of slaughter weight and lifespan of dairy cows, which could be considered as surrogates of clinical disease.Additional key words: age; dairy cattle; Johne's disease, lifespan; meat; Mycobacterium; slaughter weight. ResumenEfectos de la paratuberculosis bovina sobre el peso de la canal y la edad de desvieje en ganado Frison La paratuberculosis bovina (PTB) ocasiona grandes pérdidas económicas en las explotaciones de vacuno lechero a causa del descenso de la producción láctea, la pérdida de peso y el sacrificio prematuro de los animales infectados. El objetivo de este estudio fue evaluar el efecto de la infección por Mycobacterium avium subspecies paratuberculosis (MAP) en el peso neto canal y la edad media al desvieje en el ganado Frisón. En el estudio se incluyeron 1014 vacas adultas sacrificadas en dos mataderos del País Vasco. La infección por MAP fue evaluada mediante varias técnicas: ELISA indirecto a partir de muestras de sangre, aislamiento de MAP de tejidos mediante cultivo y PCR a tiempo real (rtPCR) y examen histopatológico. Las interpretaciones en serie y en paralelo fueron estimadas para determinar las tasas de infección mínimas y máximas. En el 58,9% de los animales se detectó la infección por MAP mediante alguna de estas técnicas. La histopatología (46,9%) y la rtPCR (29,6%) detectaron la mayoría de las vacas infectadas. Las pérdidas de peso neto canal se estimaron en el 3,7% si se observaban lesiones inflamatorias compatibles con PTB y 12,4% si se detectaban anticuerpos anti-MAP. La ...

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“…Although none of the vaccines induced sterile immunity they did slow disease progression and demonstrated that development of a vaccine that induces sterile immunity is feasible (reviewed in (Rosseels and Huygen 2008). Of interest, the studies also revealed the current vaccines can be used therapeutically to reverse immunopathology (Bull et al 2007;Juste et al 2009;Singh et al 2010), an observation first observed by Koch (reviewed in (Burke 1993)). If animals are not too far advanced in clinical disease, the effect can be dramatic with a reversal of pathology (Singh et al 2010).…”

Section: Various Approaches Have Been Taken To Develop a Vaccine Tomentioning

confidence: 88%

“…paratuberculosis (Map) that induces sterile immunity against Johne's disease (JD) or prevents disease progression. These approaches have included development of killed whole vaccines, live attenuated, DNA and subunit vaccines and tests in different animal species (Bull et al 2007;Park et al 2008;Juste et al 2009;Kathaperumal et al 2009;Scandurra et al 2010;Bastida and Juste 2011;Stabel et al 2012;Faisal et al 2013). Although none of the vaccines induced sterile immunity they did slow disease progression and demonstrated that development of a vaccine that induces sterile immunity is feasible (reviewed in (Rosseels and Huygen 2008).…”

Section: Various Approaches Have Been Taken To Develop a Vaccine Tomentioning

confidence: 99%

Therapeutic Potential of a Candidate relA Deletion Mutant Vaccine of Mycobacterium Avium Subsp. Paratuberculosis

Park¹

2014

Adv. Anim. Vet. Sci.

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Significant reduction in bacterial shedding and improvement in milk production in dairy farms after the use of a new inactivated paratuberculosis vaccine in a field trial

Cited by 53 publications

References 9 publications

Latent infections are the most frequent form of paratuberculosis in slaughtered Friesian cattle

Latent infections are the most frequent form of paratuberculosis in slaughtered Friesian cattle

Effects of paratuberculosis on Friesian cattle carcass weight and age at culling

Therapeutic Potential of a Candidate relA Deletion Mutant Vaccine of Mycobacterium Avium Subsp. Paratuberculosis

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