Relatively little is known about the locus of boron in the plant cell or its availability for movement from one site to another. It appears that little or no reutilization of boron occurs in dicotyledonous plants, and an external available supply is needed throughout the period of growth. As the borate ion complexes readily with various polyhydroxy compounds (13), localization of boron in certain cellular fractions might not necessarily imply an important physiological role at these sites; nevertheless, information on its distribution or localization within the plant cell could contribute to a better understanding of its function.Boron determinations on various intracellular fractions separated by differential centrifugation indicated certain consistent trends regarding the distribution of boron within the cell, particularly in relation to growth, boron utilization and boron availability. These results have been briefly summarized (12) and will be reported fully here.
MATERIALS AND METHODSSunflower (Helianthus annuus L., var. Mammoth Russian) and mung bean (Phaseolus aureus Roxb.) were grown in nutrient solutions in a controlledenvironment room under conditions previously described (10). The seeds were sown in quartz sand, watered with tap water, and one week later the seedlings were transferred to nutrient solutions contained in wide-mouth, low-boron soft glass quart fruit jars wrapped in black cloth. The sunflower plants were grown in solutions of the macronutrient composition previously described (10); the solutions for the mung beans consisted of a one-eighth concentration of Hoagland and Arnon's no. 1 solution (3). Iron, manganese, zinc and copper were added to both solutions at concentrations of 5.0, 0.5, 0.05 and 0.02 ppm respectively.As Plants were harvested at several periods for various determinations. Twenty-five g fresh tissue samples were used; this included the terminal bud regions and the blades of the two youngest, fully expanded leaves of about 11 plants for sunflower and the terminal bud regions and the leaflets from the youngest, fully expanded leaf of about 45 plants for mung bean. The samples were prepared and separated into various intracellular fractions by the procedure used bv Gordon (2). The tissue was ground in a chilled mortar with the aid of acid-washed sand in 50 ml phosphate buffer, 0.2 M, of pH 7.0, made 0.3 M with sucrose. The homogenate was strained through 200-mesh nylon cloth; the material that passed through is designated homogenate filtrate and that remaining in the cloth as homogenate residue (