Silanization of Porous Silicon Surface for Ion-Sensor Applications

Meskini, O.; Tlili, A.; Mghaieth, Ridha; Jaffrézic‐Renault, Nicole; Abdelghani, Adnane

doi:10.1166/sl.2009.1172

Cited by 4 publications

(4 citation statements)

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“…In light of the above, there are a number of avenues to explore in order to move towards calibration-free measurements. First, molecules must be covalently grafted, which can be carried out using silanization [ 29 ]. The SNR must be high enough to allow the construction of an accurate model.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence Spectra of Prototropic Forms of Fluorescein and Some Derivatives and Their Potential Use for Calibration-Free pH Sensing

Gauthier-Manuel,

Benmouhoub,

Wacogne

2024

Sensors

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Fluorescence pH sensing has proven to be efficient but with the drawback that molecules photobleach, requiring frequent calibrations. Double-emission peak molecules allow ratiometric measurements and theoretically avoid calibration. However, they are often expensive and fragile and usually have very low quantum yields. Single emission peaks such as fluorescein and derivatives are inexpensive and have very high quantum yields. Because they are single emission peaks, the pH is assumed to be derived from the ratio of emitted intensities at measured pH and at high pH values, i.e., they require frequent calibration. However, the shape of their single emitted peak evolves slightly with pH. In this paper, we first demonstrate a simple method to calculate the emission spectrum shape of each prototropic form of fluorescein (and derivatives) as well as the values of the pKas. A complete model of the evolution of the emission spectrum shape with pH is then constructed. Second, we evaluate the potential of these molecules for pH sensing by fitting the experimental spectra with the complete emission model. The method is applied to fluorescein, FITC and FAM. Depending on the molecule, pH can be measured from pH 1.9 to pH 7.3 with standard deviations between 0.06 and 0.08 pH units. Estimating pH and pKas from shape instead of intensity allows calibration-free measurements even with single-emission peak molecules.

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Section: Discussionmentioning

confidence: 99%

Fluorescence Spectra of Prototropic Forms of Fluorescein and Some Derivatives and Their Potential Use for Calibration-Free pH Sensing

Gauthier-Manuel,

Benmouhoub,

Wacogne

2024

Sensors

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“…For certain types of functionalization chemistries, amine groups located at the end of molecules which are grafted onto the sensor's surface are used to covalently attach ligands onto the surface. Depending on the sensor surface material, different chemistries can be used; thiol chemistry for gold coated surfaces [1] or silane chemistry for glass substrates [2]. In most cases, functionalization leads to the creation of a monolayer of amine terminated molecules.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity enhancement of a fluorescent pH sensor by double silanization of the sensing surface

Gauthier‐Manuel

Kateklum

Pieralli

et al. 2019

Materials Today: Proceedings

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The goal of this work concerns a new method to substantially increase the sensitivity of a fluorescence pH sensor. The method is based on a double silanization method. Two methods are compared: one using APTMS (3-Aminopropyl)trimethoxysilane) only and another one using both APTMS and APDMS (3-Aminopropyl)dimethoxymethylsilane). Using the second method, sensor's sensitivity is improved by more than 500 %.

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“…These amine groups are generally located at the end of molecules which are grafted onto the sensor surface. Depending on the sensor surface material, different chemistries can be used; thiol chemistry for gold coated surfaces (for SPR experiments for example [1]) or silane chemistry for glass substrates (for ion sensing for example [2]). However, regardless of the type of chemistry used, functionalization often leads to the creation of a monolayer of amine terminated molecules.…”

Section: Introductionmentioning

confidence: 99%

Improving the sensitivity of amino-silanized sensors using self-structured silane layers: Application to fluorescence pH measurement

Kateklum

Gauthier‐Manuel

Pieralli

et al. 2017

Sensors and Actuators B: Chemical

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We investigated the possibility to grow molecularly porous amino-silane layers on glass-like substrates. The goal of this work is to show that it is possible to substantially increase the sensitivity of a fluorescence sensor by adjusting the functionalization strategy. Two methods are studied, one using APTMS only and another one using both APTMS and APDMS. We show that, using the second method, sensor sensitivity is improved by a factor of about 5. In order to demonstrate this, we applied the technique to the grafting of fluorescein in order to build a fluorescence pH sensor.

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Silanization of Porous Silicon Surface for Ion-Sensor Applications

Cited by 4 publications

References 0 publications

Fluorescence Spectra of Prototropic Forms of Fluorescein and Some Derivatives and Their Potential Use for Calibration-Free pH Sensing

Fluorescence Spectra of Prototropic Forms of Fluorescein and Some Derivatives and Their Potential Use for Calibration-Free pH Sensing

Sensitivity enhancement of a fluorescent pH sensor by double silanization of the sensing surface

Improving the sensitivity of amino-silanized sensors using self-structured silane layers: Application to fluorescence pH measurement

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