Silencing of circ_0078607 prevents development of gastric cancer and inactivates the ERK1/2/AKT pathway through the miR-188-3p/RAP1B axis

Bian, Weixin; Liu, Zhiqiang; Chu, Yanjie; Xing, Xiaojing

doi:10.1097/cad.0000000000001083

Cited by 7 publications

(6 citation statements)

References 27 publications

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“…The competing endogenous RNA (ceRNA) theory suggested that circRNA regulated gene expression through sponge miRNAs 1 . Previous studies had shown that miR‐188‐3p played an important role in cancers such as hepatocellular carcinoma, 24 breast cancer, 25 and gastric cancer 26 . In PE, miR‐188‐3p had been reported to be down‐regulated, but its effect on PE has not been investigated 21 .…”

Section: Discussionmentioning

confidence: 99%

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Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR‐188‐3p/GRHL2 axis

Zhou

Lou

et al. 2022

American J Rep Immunol

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Problem: Preeclampsia (PE) is the main factor threatening the life of primipara.Defective migration and invasion of trophoblast cells was one of the causes of PE.Circ_0111277 had been reported to be related to the development of PE, but the mechanism of its effect on trophoblast cells needed further study. Method of Study:The expression of circ_0111277, and grainyhead-like 2 (GRHL2) mRNA were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit-8 (CCK-8) assay, 5-Ethynyl-20deoxyuridine (EdU) and colony formation assay were used to examine cell proliferation ability. Tube formation and transwell assay were performed to assess the angiogenesis and metastasis ability of cells. Western blot was applied to measure the levels of epithelial-mesenchymal transition (EMT)-related proteins (E-cadherin and Vimentin) and GRHL2 protein. The relationship between miR-188-3p and circ_0111277 or GRHL2 was verified by the dual luciferase reporter experiment.Results: Circ_0111277 and GRHL2 were elevated, and miR-188-3p was declined in PE patients. Overexpression of circ_0111277 could inhibit the proliferation, angiogenesis, migration, invasion and EMT of trophoblast cells (HTR-8/Svneo). Circ_0111277 was the molecular sponge of miR-188-3p. MiR-188-3p up-regulation could reduce the inhibition of HTR-8/Svneo cell growth caused by overexpression of circ_0111277. GRHL2 was a target gene of miR-188-3p, and GRHL2 silencing relieved the adverse effects of miR-188-3p inhibitors on HTR-8/Svneo. In general, circ_0111277 up-regulated GRHL2 expression through sponge miR-188-3p. Conclusion:Highly expressed circ_0111277 up-regulated the expression of GRHL2 through sponge miR-188-3p, thereby inhibiting trophoblast cells function, which suggested a new molecular mechanism for the pathogenesis of PE.Meixi Zhou and Xiang Yu contributed equally to this work.

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Section: Discussionmentioning

confidence: 99%

“…1 Previous cers such as hepatocellular carcinoma, 24 breast cancer, 25 and gastric cancer. 26 In PE, miR-188-3p had been reported to be down-regulated, but its effect on PE has not been investigated. 21 Our results also confirmed that the expression level of miR-188-3p in PE placenta was lower than that in normal placenta.…”

Section: Discussionmentioning

confidence: 99%

Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR‐188‐3p/GRHL2 axis

Zhou

Lou

et al. 2022

American J Rep Immunol

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“…The network analysis indicated that the ERK1/2/AKT pathway was involved in the metastasis of gastric cancer. 67 Circ-Rangap1 was found to be highly expressed in the plasma of patients with gastric cancer. Its high expression was positively correlated with TNM stage and advanced lymph node metastasis.…”

Section: Gastric Cancermentioning

confidence: 99%

Plasma circRNAs as Biomarkers in Cancer

Zhou¹,

Ju²,

Ji³

et al. 2021

CMAR

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The incidence and mortality of cancer are increasing each year. At present, the sensitivity and specificity of the blood biomarkers that were used in clinical practice are low, which make the detection rate of cancer decrease. With advances in bioinformatics and technology, some non-coding RNA as biomarkers can be easily detected through some traditional and new technologies. Circular RNAs (circRNAs) are non-coding RNAs, that is, they do not encode proteins, and have important regulatory functions. CircRNAs can remain stable in bodily fluids, such as in saliva, blood, urine, and especially plasma. The difference in the expression of plasma circRNAs between cancer patients and normal people may suggest that plasma circRNAs may play an important role in the occurrence and development of cancer. In this review, we summarized the clinical effect of plasma circRNAs in several high-incidence cancers. CircRNAs may be effective biomarkers for tumour diagnosis, treatment selection and prognosis evaluation.

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“…Circ_0078607 has been reported to control tumorigenesis and regulate proliferation and migration in human cancers. Downregulation of circ_0078607 blocked the gastric cancer development and induced the inactivation of the ERK1/2 and Akt pathways via targeting the miR-188-3p and RAP1B ( Bian et al, 2021 ). Circ_0078607 has been found to repress tumor progression via modulating the miR-518a-5p and Fas signaling pathway in ovarian cancer ( Zhang et al, 2020c ).…”

Section: Role Of Circrnas In Drug Resistancementioning

confidence: 99%

The role of circRNAs in regulation of drug resistance in ovarian cancer

Zhan,

Li,

Lin

et al. 2023

Front. Genet.

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Ovarian cancer is one of the female reproductive system tumors. Chemotherapy is used for advanced ovarian cancer patients; however, drug resistance is a pivotal cause of chemotherapeutic failure. Hence, it is critical to explore the molecular mechanisms of drug resistance of ovarian cancer cells and to ameliorate chemoresistance. Noncoding RNAs (ncRNAs) have been identified to critically participate in drug sensitivity in a variety of human cancers, including ovarian cancer. Among ncRNAs, circRNAs sponge miRNAs and prevent miRNAs from regulation of their target mRNAs. CircRNAs can interact with DNA or proteins to modulate gene expression. In this review, we briefly describe the biological functions of circRNAs in the development and progression of ovarian cancer. Moreover, we discuss the underneath regulatory molecular mechanisms of circRNAs on governing drug resistance in ovarian cancer. Furthermore, we mention the novel strategies to overcome drug resistance via targeting circRNAs in ovarian cancer. Due to that circRNAs play a key role in modulation of drug resistance in ovarian cancer, targeting circRNAs could be a novel approach for attenuation of chemoresistance in ovarian cancer.

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Silencing of circ_0078607 prevents development of gastric cancer and inactivates the ERK1/2/AKT pathway through the miR-188-3p/RAP1B axis

Cited by 7 publications

References 27 publications

Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR‐188‐3p/GRHL2 axis

Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR‐188‐3p/GRHL2 axis

Plasma circRNAs as Biomarkers in Cancer

The role of circRNAs in regulation of drug resistance in ovarian cancer

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