Silencing of keratin 17 by lentivirus‑mediated short hairpin RNA inhibits the proliferation of PANC‑1 human pancreatic cancer cells

Chen, Peng; Shen, Zhengchao; Fang, Xiaosan; Wang, Guannan; Wang, Xiaoming; Wang, Jun; Xi, Shihang

doi:10.3892/ol.2020.11469

Cited by 8 publications

(15 citation statements)

References 47 publications

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“…Intriguingly, when we analyzed the downstream pathways of KRT17, we noticed that the ablation of KRT17 also leads to the deactivation of both AKT and ERK pathway. It was reported that KRT17 deficiency impairs the activation of ERK, while makes little difference on AKT activation in pancreatic cancer cells [21], which disagrees with our present results. However, a recent in vitro and in vivo study on osteosarcoma cells have shown that KRT17 participates in the regulation of the AKT/mTOR/HIFα pathway [32].…”

Section: Discussioncontrasting

confidence: 99%

“…For instance, KRT17 ablation suppresses the proliferation of oral squamous cell carcinoma in vitro and in vivo in nude mice [24]. Similarly, the pro-proliferation function of KRT17 was also observed in pancreatic cancer cell line [21]. It was demonstrated that pancreatic cancer cells transfected with KRT17 siRNA showed lower Reactive-Oxygen-Species and mTOR/S6K1 phosphorylation levels, as well as reduced proliferation, migration and invasion [25].…”

Section: Discussionmentioning

confidence: 93%

“…It has been reported previously that KRT17 silencing inactivates ERK1/2 but not AKT pathway in pancreatic cancer cells [21]. Therefore, we tested whether the same mechanism of KRT17 action is involved in BCa.…”

Section: Krt17 Regulated Akt and Erk Pathwaymentioning

confidence: 90%

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Keratin 17 knockdown suppressed malignancy and cisplatin tolerance of bladder cancer cells, as well as the activation of AKT and ERK pathway

Ruan

et al. 2021

Folia Histochem Cytobiol.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 93%

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Keratin 17 knockdown suppressed malignancy and cisplatin tolerance of bladder cancer cells, as well as the activation of AKT and ERK pathway

Ruan

et al. 2021

Folia Histochem Cytobiol.

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“… 39 In addition, Chen et al discovered that inhibition of the proliferation of PANC‑1 human pancreatic cancer cells could be replicated by silencing of KRT17 through lentivirus‑mediated short hairpin RNA. 40 And it was found that upregulation of KRT17 promoted PDAC cell proliferation, invasion, and metastasis through EMT. 40 In addition, some studies raised concerns that overexpression of KRT17 mRNA was associated with a poor outcome for PDAC patients.…”

Section: Discussionmentioning

confidence: 99%

“… 40 And it was found that upregulation of KRT17 promoted PDAC cell proliferation, invasion, and metastasis through EMT. 40 In addition, some studies raised concerns that overexpression of KRT17 mRNA was associated with a poor outcome for PDAC patients. 41 In our study, RNA-seq analysis demonstrated that mRNA expression of KRT17 was markedly increased in cancer tissues compared to adjacent normal tissue.…”

Section: Discussionmentioning

confidence: 99%

Identification of Critical Pathways and Potential Key Genes in Poorly Differentiated Pancreatic Adenocarcinoma

Lu¹,

Li²,

Liu³

et al. 2021

OTT

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Introduction The poorly differentiated pancreatic adenocarcinoma (PDAC) is an extremely lethal neoplasm without effective biomarkers for early detection and prognosis prediction, which is characteristically unresponsive to chemotherapeutic regimens. This study aims at searching for key genes which could be applied as novel prognostic biomarkers and therapeutic targets in PDAC. Methods Clinical samples were collected and a comprehensive differential analysis of seven PDAC samples by integrating RNA-seq data of tumor tissues and matched normal tissues from both our cohort and gene expression profiling interactive analysis (GEPIA) were performed to discover potential prognostic genes in PDAC. Pathway enrichment analysis was carried out to determine the biological function of PDAC differentially expressed genes (DEGs), and protein-protein interaction (PPI) network was constructed for functional modules analysis. Real-time PCR was performed to validate expression of hub genes. Results A total of 126 PDAC-specific expressed genes identified from seven PDAC samples were predominantly enriched in cell adhesion, integral component of membrane, signal transduction and chemical carcinogenesis, IL-17 signaling pathway, indicating that obtained genes might play a unique role in PDAC tumorigenesis. Furthermore, survival analysis revealed that five genes ( CEACAM5 , KRT6A , KRT6B , KRT7 , KRT17 ) which exhibited high expression levels in tumor tissues were obviously correlated with the prognosis of PDAC patients and KRT7 was positively correlated with KRT6A , KRT6B , KRT17 expression. In addition, real-time PCR demonstrated that the expression level of the hub genes was consistent with RNA-seq analysis. Discussion The current study suggested that CEACAM5 , KRT6A , KRT6B , KRT7 , and KRT17 may represent novel prognostic biomarkers as well as novel therapeutic targets for poorly differentiated PDAC.

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Solasodine, Isolated from Solanum sisymbriifolium Fruits, Has a Potent Anti-Tumor Activity Against Pancreatic Cancer

Fan

et al. 2021

DDDT

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Background Increasing evidences have revealed that solasodine, isolated from Solanum sisymbriifolium fruits, has multiple functions such as anti-oxidant, anti-tumor and anti-infection. However, its role in pancreatic cancer has not been well studied. Methods To explore the role of solasodine in pancreatic cancer, human pancreatic cell lines including SW1990 and PANC1 were treated with different concentrations of solasodine for 48 h, and cell viability was evaluated by MTT assay, cell invasion and migration were evaluated by Transwell assay. The effect of solasodine on the apoptosis of SW1990 and PANC1 cells was detected by flow cytometry. To further explore the antitumor effect of solasodine in vivo, an SW1990 tumor-bearing mouse model was constructed. The effects of solasodine on cytokines in the serum of SW1990 tumor-bearing mice were also evaluated by ELISA assay. Results Specifically, in vitro, solasodine could significantly inhibit the proliferation of pancreatic cancer cell lines SW1990 and PANC1 cells. Flow cytometric analysis indicated that solasodine could induce apoptosis of SW1990 and PANC1 cells. Western blot assay indicated that solasodine could significantly inhibit the activation of Cox-2/Akt/GSK3β signal pathway. Meanwhile, the release of Cytochrome c from mitochondria to cytoplasm which can raise the caspases cascade (C-caspase 3 and C-caspase 9) was significantly enhanced by solasodine. In vivo, the results showed that solasodine had potent anti-tumor activities with a lower cytotoxicity. In addition, the serum TNF-α, IL-2 and IFN-γ levels in SW1990 tumor-bearing mice after the treatment of solasodine was significantly increased. Conclusion Taken together, our results suggested that the solasodine could prevent the progression of pancreatic cancer by inhibiting proliferation and promoting apoptosis, as well as stimulating immunity, suggesting that solasodine might be a potential therapeutic strategy for pancreatic cancer.

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Silencing of keratin 17 by lentivirus‑mediated short hairpin RNA inhibits the proliferation of PANC‑1 human pancreatic cancer cells

Cited by 8 publications

References 47 publications

Keratin 17 knockdown suppressed malignancy and cisplatin tolerance of bladder cancer cells, as well as the activation of AKT and ERK pathway

Keratin 17 knockdown suppressed malignancy and cisplatin tolerance of bladder cancer cells, as well as the activation of AKT and ERK pathway

Identification of Critical Pathways and Potential Key Genes in Poorly Differentiated Pancreatic Adenocarcinoma

Solasodine, Isolated from Solanum sisymbriifolium Fruits, Has a Potent Anti-Tumor Activity Against Pancreatic Cancer

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