Silencing of MALAT1 inhibits migration and invasion by sponging miR‑1‑3p in prostate cancer cells

Abstract

Prostate cancer is a common malignancy with a high mortality rate. Long non-coding RNA metastasis associated with lung adenocarcinoma transcript 1 (MALAT1) has been reported to serve tumor-promoting roles. However, the underlying mechanism requires further examination. In the present study, it was demonstrated that MALAT1 was increased while microRNA (miR/miRNA)-1-3p was decreased in prostate cancer cell lines. The silencing of MALAT1 inhibited migration, invasion and epithelial-mesenchymal transition, when epithelial (E)-cadherin expression level was increased, and neural (N)-cadherin, vimentin, Slug and Snail expression levels were decreased. Dual-luciferase reporter assay results demonstrated that miR-1-3p bound to MALAT1 and coronin 1C (CORO1C) 3′ untranslated region, and MALAT1 competed with CORO1C for the binding sites of miR-1-3p. MALAT1 inhibited the expression of miR-1-3p and vice versa. MALAT1 knockdown induced the decline of CORO1C, which was subsequently recovered by the miR-1-3p inhibitor. In addition, by inhibiting miR-1-3p or overexpressing CORO1C, the silencing of MALAT1-induced phenotypic alterations were restored. In conclusion, MALAT1 serving as a degradable miRNA sponge, may sequester miR-1-3p from CORO1C and by silencing MALAT1, migration, invasion and epithelial-mesenchymal transition may be inhibited in prostate cancer cells. MALAT1 and CORO1C may serve as novel clinical therapeutic targets for prostate cancer.

<p>MALAT1 Promotes Cell Tumorigenicity Through Regulating miR-515-5p/EEF2 Axis in Non-Small Cell Lung Cancer</p>

“…29 Consistently, MALAT1 knockdown restrained the metastasis of prostate cancer cells by directly interacting with miR-1-3p. 30 Therefore, it is imperative to disclose exosomal-derived MALAT1 might be involved in the tumorigenesis of NSCLC cells.…”

Association of rs3746444 in MiRNA-499 and rs3787016 in Long Non-coding RNAs POLR2E with Prostate Cancer: an updated meta-analysis and systematic review

“…Acting as the precursors of small non-coding RNAs, LncRNAs can produce MicroRNAs as well as endogenous small interfering RNA or served as a microRNA sponge to inhibit microRNA activity [26,27]. Meanwhile, several LncRNA also play vital roles, activating or suppressing oncogenes [28,29].…”

Interplay among SNAIL Transcription Factor, MicroRNAs, Long Non-Coding RNAs, and Circular RNAs in the Regulation of Tumor Growth and Metastasis

“…MALAT1 turned out to be a regulator of not only miR-22, but also miR-1-3p expression. In that way, it inhibits migration, invasion, and EMT, which leads to the increased expression of E-cadherin and decreased expression of vimentin, SLUG, and SNAIL [132]. Another interesting feature of MALAT1 is the modulation of cancer stem cells' (CSC) activity by regulation of the miR-1/SLUG axis in nasopharyngeal carcinoma [133].…”

“…This mechanism may be responsible for the regulation of tumor progression. miR-22 and miR-1-3p E-cadherin, vimentin, SLUG and SNAIL prostate cancer [132] miR-1 SLUG nasopharyngeal carcinoma [133] lncRNA H19 miR-22-3p SNAIL gastric cancer [134] lncRNA SNHG7 miR-34a SNAIL gastric cancer [135] lncRNA CAR10 miR-30 and miR-203 SNAIL and SLUG lung adenocarcinoma [137] lncRNA HCP5 miR-203 SNAIL lung adenocarcinoma [138] lncRNA UCA1 miR-203 SLUG hepatocellular carcinoma [139] lncRNA AB209371 miR199a-5p SNAIL hepatocellular carcinoma [140] lncRNA TINCR miR-125b SNAIL breast cancer [85] lncRNA SATB2-AS1 -SNAIL (epigenetic regulation involving SATB2) colorectal cancer [141] lncRNA NEAT1 -E-cadherin by association with G9a-DNMT1-SNAIL complex osteosarcoma cells [142] lncRNA SNHG15 -SNAIL (ubiquitination by interaction with zinc finger domain) colon cancer [143] lncRNA GAPLINC -SLUG (by binding to PSF/NONO) colorectal cancer [136] circ-ZNF652 miR-203 and miR-502-5p SNAIL hepatocellular carcinoma [96] circRNA_0084043 miR-153-3p SNAIL melanoma [144] circRNA PRMT5 miR-30c SNAIL urothelial carcinoma [145] circRNA-000284 miR-506 SLUG cervical cancer [146] hsa_circ_0008305 (circPTK2) miR-429 and miR-200b-3p SNAIL (indirectly by TIF1γ) non-small cell lung cancer [147] circPIP5K1A miR-600 SNAIL (indirectly by HIF-1α) non-small cell lung cancer [148] circ_0026344 miR-183 SNAIL (indirectly) colorectal cancer [149]…”