Silencing of phospholipase C gamma 2 promotes proliferation of rat hepatocytes in vitro

Chen, Xiaoguang; Zhu, Xuemin; Liu, Yumei; Lv, Qiongxia; Ma, Jun

doi:10.1002/jcb.26592

Cited by 4 publications

(3 citation statements)

References 46 publications

(65 reference statements)

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“…PLCB2 regulates the function of the hepatic endothelial barrier and mediates intracellular signaling downstream of G protein-coupled receptors. In previous research, an increase in phospholipase C gamma 2 ( PLCγ2 ) mRNA was detected in the late phase of rat liver regeneration [ 96 ]. Although, TNF-α alone cannot induce apoptosis in normal hepatic tissue, because TNF-α also activates antiapoptotic signal pathways.…”

Section: Discussionmentioning

confidence: 99%

Hepatic transcript profiling in beef cattle: Effects of rumen-protected niacin supplementation

et al. 2023

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The objective of our study was to assess the effect of rumen-protected niacin supplementation on the transcriptome of liver tissue in growing Angus × Simmental steers and heifers through RNA-seq analysis. Consequently, we wanted to assess the known role of niacin in the physiological processes of vasodilation, detoxification, and immune function in beef hepatic tissue. Normal weaned calves (~8 months old) were provided either a control diet or a diet supplemented with rumen-protected niacin (6 g/hd/d) for a 30-day period, followed by a liver biopsy. We observed a significant list of changes at the transcriptome level due to rumen-protected niacin supplementation. Several metabolic pathways revealed potential positive effects to the animal’s liver metabolism due to administration of rumen-protected niacin; for example, a decrease in lipolysis, apoptosis, inflammatory responses, atherosclerosis, oxidative stress, fibrosis, and vasodilation-related pathways. Therefore, results from our study showed that the liver transcriptional machinery switched several metabolic pathways to a condition that could potentially benefit the health status of animals supplemented with rumen-protected niacin. In conclusion, based on the results of our study, we can suggest the utilization of rumen-protected niacin supplementation as a nutritional strategy could improve the health status of growing beef cattle in different beef production stages, such as backgrounding operations or new arrivals to a feedlot.

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Section: Discussionmentioning

confidence: 99%

Hepatic transcript profiling in beef cattle: Effects of rumen-protected niacin supplementation

et al. 2023

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“…In combination with various databases and preliminary screening results, we selected three typical mRNAs for further verification. For example, PLCG2 could promote proliferation through inactivating ERK and NF-κB pathway ( Ma et al, 2019 ), p38 MAPK and JNK MAPK pathways ( Chen et al, 2018a ), PKCδ-mediated JNK1/2 signaling pathway ( Chen et al, 2018b ). The TPR contributes to the organization of the nuclear lamina and in cooperation with lamins guards the interphase assembly of nuclear pore complexes ( Fišerová et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of N6-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction

Huang

et al. 2022

Front. Genet.

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Background: From previous studies, we found that there are more than 100 types of RNA modifications in RNA molecules. m6A methylation is the most common. The incidence rate of adenocarcinoma of the esophagogastric junction (AEG) at home and abroad has increased faster than that of stomach cancer at other sites in recent years. Here, we systematically analyze the modification pattern of m6A mRNA in adenocarcinoma at the esophagogastric junction.Methods: m6A sequencing, RNA sequencing, and bioinformatics analysis were used to describe the m6A modification pattern in adenocarcinoma and normal tissues at the esophagogastric junction.Results: In AEG samples, a total of 4,775 new m6A peaks appeared, and 3,054 peaks disappeared. The unique m6A-related genes in AEG are related to cancer-related pathways. There are hypermethylated or hypomethylated m6A peaks in AEG in differentially expressed mRNA transcripts.Conclusion: This study preliminarily constructed the first m6A full transcriptome map of human AEG. This has a guiding role in revealing the mechanism of m6A-mediated gene expression regulation.

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“…27 PLCγ2 gene expression inhibition can restrain the MEKK1/MKK4/JNK1/2/c-Jun signaling pathway to induce caspase-dependent cell death. 28 Nevertheless, the function of the pathway in cardiovascular diseases has not hitherto been reported.…”

Section: Introductionmentioning

confidence: 99%

Exosomal miR-455-3p from BMMSCs prevents cardiac ischemia-reperfusion injury

Wang

Shen

2022

Hum Exp Toxicol

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Objective Bone marrow mesenchymal stem cells (BMMSCs) exert protective effects against myocardial infarction (MI). Here, we focused on the function and mechanism of miR-455-3p from BMMSCs-derived exosomes (BMMSCs-Exo) in myocardial infarction. Materials and methods BMMSCs were isolated from rat bone marrow, and the exosomes from the culture medium of BMMSCs were separated, and administered to H9C2 cells under hypoxia-reperfusion (H/R) stimulation. MTT and TUNEL staining analyzed cell viability and apoptosis, respectively. RT-qPCR determined miR-455-3p expression. Apoptosis-related proteins, autophagy-associated proteins, and the MEKK1-MKK4-JNK signaling pathway were detected. The interaction between miR-455-3p and MEKK1 was confirmed through dual luciferase activity and RIP assay. An in vivo ischemia reperfusion (I/R) model was established in rats. 2, 3, 5 triphenyltetrazolium chloride (TTC) staining, hematoxylin-eosin (H&E) staining, Masson staining, and TUNEL staining evaluated the infarct volume and histopathological changes. Results miR-455-3p′s expression was down-regulated in BMMSCs-derived exosomes, I/R myocardial tissues, and H/R myocardial cells. miR-455-3p enriched by BMMSC exosomes reduced H/R-mediated cardiomyocyte damage and death-related autophagy. miR-455-3p upregulation suppressed MEKK1-MKK4-JNK. MEKK1 overexpression notably mitigated cell apoptosis, cramped cell viability, suppressed autophagy expansion, and attenuated Exo-miR-455-3p′s protection on H/R myocardial cells. In-vivo trials reflected that BMMSC exosomes enriched with miR-455-3p repressed ischemia reperfusion-induced myocardial damage and myocardial cell function. Conclusion miR-455-3p, shuttled by exosomes from MSCs, targets the MEKK1-MKK4-JNK signaling pathway to guard against myocardial ischemia-reperfusion damage.

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Silencing of phospholipase C gamma 2 promotes proliferation of rat hepatocytes in vitro

Cited by 4 publications

References 46 publications

Hepatic transcript profiling in beef cattle: Effects of rumen-protected niacin supplementation

Hepatic transcript profiling in beef cattle: Effects of rumen-protected niacin supplementation

Analysis of N6-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction

Exosomal miR-455-3p from BMMSCs prevents cardiac ischemia-reperfusion injury

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