Silencing of SlFTR-c, the catalytic subunit of ferredoxin:thioredoxin reductase, induces pathogenesis-related genes and pathogen resistance in tomato plants

Lim, Chan Ju; Kim, Woong Bom; Lee, Bok-Sim; Lee, Ha Youn; Kwon, Taeho; Park, Jeong Mee; Kwon, Suk‐Yoon

doi:10.1016/j.bbrc.2010.08.016

Cited by 17 publications

(19 citation statements)

References 49 publications

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“…To gain further information concerning the increased salt tolerance phenotype observed in SlDEAD31 -overexpressing tomato plants, transcripts of previously reported stress-related genes were utilized for comparative analysis between WT and transgenic tomato roots under normal and salt-stressed conditions. The key ascorbic acid (AsA) synthetase gene GME2 [ 30 ]; two catalase (CAT) genes, Cat1 and Cat2 [ 31 ]; an ascorbate peroxidase (APX) gene, APX2 [ 32 ]; an ethylene-responsive LEA gene ( ER5 ) [ 33 ]; the ethylene-responsive factor ERF1 [ 34 ], and two pathogenesis-related (PR) genes, PR1 and PR5 [ 35 ] were selected ( Fig 7 ). Based on the reported literature, these stress tolerance genes are involved in the regulation of biotic and abiotic stress responses (such as PR1 , PR5 , ER5 , and ERF1 ), production of osmolytes (such as GME2 ), detoxification and redox homeostasis (such as Cat1 , Cat2 , and APX2 ).…”

Section: Resultsmentioning

confidence: 99%

SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato

et al. 2015

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The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge. However, none of DEAD-box genes was well characterized in tomato so far. In this study, we reported on the identification and characterization of two putative DEAD-box RNA helicase genes, SlDEAD30 and SlDEAD31 from tomato, which were classified into stress-related DEAD-box proteins by phylogenetic analysis. Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues. Furthermore, the expression of both genes was induced mainly in roots under NaCl stress, and SlDEAD31 mRNA was also increased by heat, cold, and dehydration. In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants. Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.

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Section: Resultsmentioning

confidence: 99%

SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato

et al. 2015

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“…(a) (b) Fig. 8 Phytologist & Rey, 2006;Lim et al, 2010), is the target protein of MjTTL5 in Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

A novel nematode effector suppresses plant immunity by activating host reactive oxygen species‐scavenging system

et al. 2015

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Summary Evidence is emerging that plant‐parasitic nematodes can secrete effectors to interfere with the host immune response, but it remains unknown how these effectors can conquer host immune responses. Here, we depict a novel effector, MjTTL5, that could suppress plant immune response.Immunolocalization and transcriptional analyses showed that MjTTL5 is expressed specifically within the subventral gland of Meloidogyne javanica and up‐regulated in the early parasitic stage of the nematode. Transgenic Arabidopsis lines expressing MjTTL5 were significantly more susceptible to M. javanica infection than wild‐type plants, and vice versa, in planta silencing of MjTTL5 substantially increased plant resistance to M. javanica.Yeast two‐hybrid, coimmunoprecipitation and bimolecular fluorescent complementation assays showed that MjTTL5 interacts specifically with Arabidopsis ferredoxin : thioredoxin reductase catalytic subunit (AtFTRc), a key component of host antioxidant system. The expression of AtFTRc is induced by the infection of M. javanica. Interaction between AtFTRc and MjTTL could drastically increase host reactive oxygen species‐scavenging activity, and result in suppression of plant basal defenses and attenuation of host resistance to the nematode infection.Our results demonstrate that the host ferredoxin : thioredoxin system can be exploited cunningly by M. javanica, revealing a novel mechanism utilized by plant–parasitic nematodes to subjugate plant innate immunity and thereby promoting parasitism.

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“…Accumulation of tomato Fd‐dependent thioredoxin reductase (Sl‐FTR) was suppressed using a virus‐mediated silencing approach (Lim et al . 2010).…”

Section: Redox‐dependent Re‐adjustment Of Metabolismmentioning

confidence: 99%

“…Accumulation of tomato Fd-dependent thioredoxin reductase (Sl-FTR) was suppressed using a virus-mediated silencing approach (Lim et al 2010). The leaves of the FTRsilenced plants show necrotic lesions and accumulate ROS.…”

Section: Redox Signalling From the Ftr And Ntrc Branchmentioning

confidence: 99%

Reduction–oxidation network for flexible adjustment of cellular metabolism in photoautotrophic cells

Scheibe

Dietz

2011

Plant Cell & Environment

120

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Photosynthesis generates the energy carriers NADPH and ATP to be consumed in assimilatory processes. Continuous energy conversion and optimal use of the available light energy are only guaranteed when all reduction-oxidation (redox) processes are tightly controlled. A robust network links metabolism with regulation and signalling. Information on the redox situation is generated and transferred by various redox components that are parts of this network. Any imbalance in the network is sensed, and the information is transmitted in order to elicit a response at the various levels of regulation and in the different cellular compartments. Redox information within the chloroplast is derived from intersystem electron transport, the ferredoxin-NADP oxidoreductase (FNR)/NADPH branch of the redox network, the thioredoxin branch and from reactive oxygen species (ROS), resulting in a high diversity of responses that are able to adjust photosynthesis, as well as poising and antioxidant systems accordingly in each specific situation. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) represents a central step in CO2 reduction and in carbohydrate oxidation involving both forms of energy, namely NAD(P)H and ATP, with its various isoforms that are located in plastids, cytosol and nucleus. GAPDH is used as an example to demonstrate complexity, flexibility and robustness of the regulatory redox network in plants.

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Silencing of SlFTR-c, the catalytic subunit of ferredoxin:thioredoxin reductase, induces pathogenesis-related genes and pathogen resistance in tomato plants

Cited by 17 publications

References 49 publications

SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato

SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato

A novel nematode effector suppresses plant immunity by activating host reactive oxygen species‐scavenging system

Reduction–oxidation network for flexible adjustment of cellular metabolism in photoautotrophic cells

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