Silencing of the Nk1 gene in the SR1 Nicotiana tabacum plants by RNA interference

Influence of Septoria glycines Hemmi on the enzymes’ activity of hydrolytic Glycines max (L.) Merr seed complex

“…In most plant viruses, genetic material is represented by RNA; based on this, it can be said that extracellular RNAases induced by damage are one of antiviral protection components. Sangayev et al showed that tobacco plants with increased extracellular RNAase activity are characterized by increased resistance to tobacco mosaic virus [21]. This supports the hypothesis about the involvement of plants' extracellular RNAase in the formation of resistance to viruses.…”

Transgenic plants as genetic models for studying functions of plant genes

“…Было выдвинуто предполо-жение о возможной роли РНКаз апопласта в устойчивости к вирусам (подавляющее большинство вирусов растений содержат РНК-геномы, которые могут разрушаться рибо-нуклеазами). Для проверки этого предположения были созданы линии трансгенных растений Nicotiana tabacum, экспрессирующие гены панкреатической РНКазы Bos taurus, S-подобной РНКазы Zinnia elegans, а также линии табака с супрессированным геном собственной S-подобной РНКазы Nk1 (Сангаев и др., , 2010Trifonova et al, 2007Trifonova et al, , 2012 …”

Virus-induced silencing as a method for studying gene functions in higher plants

“…Antisense suppression of the gene activity [1][2][3] or hyperexpres sion under the control of a strong promoter (fre quently of viral origin) [4][5][6] are the most frequently used methods for the study of gene functions. How ever, both of these approaches have at least one obvi ous shortcoming (the laboriousness of transgenic plant obtainment).…”