Silica-based monolithic sensing plates for waveguide-mode sensors

Fujimaki, Makoto; Rockstuhl, Carsten; Wang, Xiaomin; Awazu, Kunio; Tominaga, Junji; Koganezawa, Yuuki; Ohki, Yoshimichi; Komatsubara, T.

doi:10.1364/oe.16.006408

Cited by 58 publications

(49 citation statements)

References 16 publications

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“…The EFC-WM sensor uses a sensing plate with a multilayer structure that consists of a dielectric waveguide, higher refractive index layer, and glass substrate [30]. The sensing plate illuminated under the Kretschmann configuration operates as a sensor that is capable of detecting modifications in the dielectric environment near the waveguide surface by measuring changes in reflectivity [40].…”

Section: Methodsmentioning

confidence: 99%

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Observations of Immuno-Gold Conjugates on Influenza Viruses Using Waveguide-Mode Sensors

et al. 2013

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Gold nanoparticles were conjugated to an antibody (immuno-AuNP) against A/Udorn/307/1972 (H3N2) influenza virus to detect viruses on a sensing plate designed for an evanescent field-coupled waveguide-mode sensor. Experiments were conducted using human influenza A/H3N2 strains, and immuno-AuNP could detect 8×105 PFU/ml (40 pg/µl) intact A/Udorn/307/1972 and 120 pg/µl A/Brisbane/10/2007. Furthermore, increased signal magnitude was achieved in the presence of non-ionic detergent, as the virtual detection level was increased to 8×104 PFU/ml A/Udorn/307/1972. Immuno-AuNPs were then complexed with viruses to permit direct observation, and they formed a ring of confined nanodots on the membrane of both intact and detergent-treated viruses as directly visualized by scanning electron microscopy. With this complex the detection limit was improved further to 8×103 PFU/ml on anti-rabbit IgG immobilized sensing plate. These strategies introduce methods for observing trapped intact viruses on the sensing plates generated for optical systems.

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Section: Methodsmentioning

confidence: 99%

“…The prism was made of SiO 2 glass, and the bottom angle of the prism was 38°C. A monolithic sensing plate that we developed previously was applied [30]. The monolithic sensing plate consisted of a SiO 2 glass substrate, single crystalline Si layer, and thermally grown SiO 2 waveguide.…”

Section: Methodsmentioning

confidence: 99%

Observations of Immuno-Gold Conjugates on Influenza Viruses Using Waveguide-Mode Sensors

et al. 2013

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“…The sensor chip had a layered structure, with a 36-nm Si layer and a 364-nm SiO 2 layer on a SiO 2 substrate (Shin-Etsu Chemical Co., Ltd., Tokyo, Japan). The method of fabrication of the sensor chip has been described in detail previously26. The SiO 2 surface of the sensor chip was modified with a self-assembled monolayer (SAM) using N-[3-(triethoxysilyl)propyl]-3,6,9,12-tetraoxatridecanamide to prevent non-specific adsorption27.…”

Section: Methodsmentioning

confidence: 99%

Detection of Extremely Low Concentrations of Biological Substances Using Near-Field Illumination

Yasuura

Fujimaki

2016

Sci Rep

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An external force-assisted near-field illumination biosensor (EFA-NI biosensor) detects a target substance that is propelled through an evanescent field by an external force. The target substance is sandwiched between an antibody coupled to a magnetic bead and an antibody coupled to a polystyrene bead. The external force is supplied by a magnetic field. The magnetic bead propels the target substance and the polystyrene bead emits an optical signal. The detection protocol includes only two steps; mixing the sample solution with a detection reagent containing the antibody-coated beads and injecting the sample mixture into a liquid cell. Because the system detects the motion of the beads, the sensor allows detection of trace amounts of target substances without a washing step. The detection capability of the sensor was demonstrated by the detection of norovirus virus-like particles at a concentration of ~40 particles per 100 μl in contaminated water.

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“…[16] [17] As reported in Synthesiology, [1] in the earliest waveguide mode sensor, the waveguide modes were formed in the ref lective film layer and the waveguide layer at a certain angle of incidence. When the angle sweep is done using the He-Ne laser at a wavelength of 632.8 nm as the light source, the reflection intensity declines at a certain angle.…”

Section: Development Of Portable Waveguide Mode Sensormentioning

confidence: 99%

Detection of influenza viruses with the waveguide mode sensor

Awazu

Fujimaki

Gopinath

et al. 2015

Synthesiology English edition

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(NA) with different antigenicity. In a new strains of influenza, mutation occurs in the HA and/or NA. Names such as type H1N1 influenza are given according to the type of HA and NA. In a new strain of influenza, new types of HA or NA appear among humans, and the antigenic drift and antigenic shift occur, or the influenza genes pass on from birds to humans. In past fact of research, it was found that the Spanish flu was caused by a hypovirulent H1N1 type derived from the avian flu.There are three reasons why a new strain of influenza is serious.(1) Since a new strain never existed before, no one has immunity. (2) It is highly infectious. It is thought that only an influenza virus that developed the ability for airborne infection causes a pandemic. (3) Since it is an unknown virus, vaccines cannot be made beforehand. Also, as an outstanding characteristic of the Spanish flu, young people developed serious conditions such as systemic infection, multiple organ failure, or a cytokine storm, which is an excessive vital reaction to viral infection. For example, the peak deaths of the Spanish flu occurred in people of ages 24-29. In Japan, there were newspaper headlines that said, "Most productive people were first to fall victims." The patients spread amongst the most productive people that supported society, and they died and paralyzed the social functions. According to the estimates by the Ministry of Health, Labour and Welfare, [5] if a pandemic equivalent to the strength of the Spanish flu (2.0 % fatality) occurred, 640 thousand people may die. However, since a mutant virus of subtype H5N1 has 60 % fatality rate, there are estimates that greater damage

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Silica-based monolithic sensing plates for waveguide-mode sensors

Cited by 58 publications

References 16 publications

Observations of Immuno-Gold Conjugates on Influenza Viruses Using Waveguide-Mode Sensors

Observations of Immuno-Gold Conjugates on Influenza Viruses Using Waveguide-Mode Sensors

Detection of Extremely Low Concentrations of Biological Substances Using Near-Field Illumination

Detection of influenza viruses with the waveguide mode sensor

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