Silica cloaking of adenovirus enhances gene delivery while reducing immunogenicity

Sapre, Ajay; Yong, Gen; Yeh, Ya san; Ruff, Laura E.; Plaut, Justin S.; Sayar, Zeynep; Agarwal, Anupriya; Martinez, Jacqueline; Nguyen, Theresa N.; Liu, Yu Tsueng; Messmer, Bradley T.; Esener, Sadik C.; Fischer, Jared M.

doi:10.1016/j.jconrel.2019.01.034

Cited by 17 publications

(16 citation statements)

References 54 publications

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“…111 Non-covalent attachment is simpler and generally obtained via attractive electrostatic interactions or incorporation in various scaffolds. This can result in different architectures such as coatings, [112][113][114] complexes, [115][116][117][118][119][120] capsules, [121][122][123][124][125] and matrices [126][127][128] as illustrated in Fig. 1.…”

Section: Physical Methodsmentioning

confidence: 99%

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Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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Section: Physical Methodsmentioning

confidence: 99%

“…466 Recently, Ad coated with silica showed enhanced TE in glioma while reducing liver accumulation and immunogenicity. 125 Other formulations of silica have also been tested. Silica-based gels are synthesized through a simple sol-gel process, resulting in highly porous materials, suitable as implantable viral depots with long-term release.…”

Section: Reviewmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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“…Moreover, Sapre and colleagues have recently reported the cloaking of adenovirus (Ad) with silica, as a way of enhancing gene delivery while reducing immunogenicity. In this work, silica-coated Ad enhanced tumor transduction by 23-fold while reducing liver uptake and off-tumor targeting by 210-fold, which minimized hepatotoxicity [ 162 ].…”

Section: Silica-based Systems As An Alternative To Other Vectorsmentioning

confidence: 99%

Silica-Based Gene Delivery Systems: From Design to Therapeutic Applications

2020

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Advances in gene therapy have been foreshadowing its potential for the treatment of a vast range of diseases involving genetic malfunctioning. However, its therapeutic efficiency and successful outcome are highly dependent on the development of the ideal gene delivery system. On that matter, silica-based vectors have diverted some attention from viral and other types of non-viral vectors due to their increased safety, easily modifiable structure and surface, high stability, and cost-effectiveness. The versatility of silane chemistry and the combination of silica with other materials, such as polymers, lipids, or inorganic particles, has resulted in the development of carriers with great loading capacities, ability to effectively protect and bind genetic material, targeted delivery, and stimuli-responsive release of cargos. Promising results have been obtained both in vitro and in vivo using these nanosystems as multifunctional platforms in different potential therapeutic areas, such as cancer or brain therapies, sometimes combined with imaging functions. Herein, the current advances in silica-based systems designed for gene therapy are reviewed, including their main properties, fabrication methods, surface modifications, and potential therapeutic applications.

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“…This hybrid drug delivery platform offers high enzyme loading efficiency, protection against proteolytic cleavage, and prolonged in-tissue stability. We outline the synthesis of enzyme-loaded core-shell PLGA NPs using a modified water-oil-water double emulsion technique (40) and the subsequent sol-gel condensation of an outer nanoporous silica layer (45)(46)(47)(48). The resultant SiLGA NPs permit diffusion of small molecule substrates into the NP core while protecting the enzymatic cargo from deactivation via proteinases and slowing burst release kinetics.…”

Section: Of 17mentioning

confidence: 99%

Hybrid Silica-Coated PLGA Nanoparticles for Enhanced Enzyme-Based Therapeutics

Gustafson¹,

Mokhtari²,

Manalo³

et al. 2022

Preprint

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Some cancer cells rely heavily on non-essential biomolecules for survival, growth, and proliferation. Enzyme based therapeutics can eliminate these biomolecules, thus specifically targeting neoplastic cells; however, enzyme therapeutics are susceptible to immune clearance, exhibit short half-lives, and require frequent administration. Encapsulation of therapeutic cargo within biocompatible and biodegradable poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) is a strategy for controlled release. Unfortunately, PLGA NPs exhibit burst release of cargo shortly after delivery or upon introduction to aqueous environments where they decompose via hydrolysis. Here we show the generation of hybrid silica-coated PLGA (SiLGA) NPs as viable drug delivery vehicles exhibiting sub-200 nm diameters, a metastable Zeta potential, and high loading efficiency and content. Compared to uncoated PLGA NPs, SiLGA NPs offer greater retention of enzymatic activity and slow the burst release of cargo. Thus, SiLGA encapsulation of therapeutic enzymes, such as asparaginase, could reduce frequency of administration, increase half-life, and improve efficacy for patients with a range of diseases.

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Silica cloaking of adenovirus enhances gene delivery while reducing immunogenicity

Cited by 17 publications

References 54 publications

Materials promoting viral gene delivery

Materials promoting viral gene delivery

Silica-Based Gene Delivery Systems: From Design to Therapeutic Applications

Hybrid Silica-Coated PLGA Nanoparticles for Enhanced Enzyme-Based Therapeutics

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