Silica-Coated Iron Oxide Nanoparticles for DNA Isolation for Molecular Genetic Studies in Hematology

Tyumentseva, Anna V.; Yaroslavtsev, R. N.; Stolyar, S. V.; Saitova, Alina T.; Tyutrina, Ekaterina S.; Gorbenko, A. S.; Stolyar, M. A.; Olkhovskiy, I. A.

doi:10.1089/gtmb.2021.0067

Cited by 2 publications

(4 citation statements)

References 15 publications

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“…However, the further use of the isolated DNA for genetic testing by the polymerase chain reaction method demonstrated the inhibitory effect of nanoparticles on the reaction enzyme (DNA polymerase). DNA isolation with 1:4.5 particles made it possible to isolate up to 3.6 times more DNA per 1 mg of sorbent, and the inhibitory effect was not observed in further studies …”

Section: Resultsmentioning

confidence: 97%

“…Some modifications in the manufacturer’s protocol of the isolation procedure were published earlier and included the absence of particle heating both at the stage of drying and at the stage of DNA elution. Elution was carried out with preheated TE buffer . The result of approbation showed that 1:1.3 nanoparticles allow the isolation of an equal or a smaller number of molecules in comparison with standard silica per 1 mg of sorbent.…”

Section: Resultsmentioning

confidence: 99%

“…Elution was carried out with preheated TE buffer. 30 The result of approbation showed that 1:1.3 nanoparticles allow the isolation of an equal or a smaller number of molecules in comparison with standard silica per 1 mg of sorbent. However, the further use of the isolated DNA for genetic testing by the polymerase chain reaction method demonstrated the inhibitory effect of nanoparticles on the reaction enzyme (DNA polymerase).…”

Section: Resultsmentioning

confidence: 99%

“…DNA isolation with 1:4.5 particles made it possible to isolate up to 3.6 times more DNA per 1 mg of sorbent, and the inhibitory effect was not observed in further studies. 30…”

Section: Resultsmentioning

confidence: 99%

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Manifestation of Stoichiometry Deviation in Silica-Coated Magnetite Nanoparticles

et al. 2022

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Iron oxide nanoparticles were synthesized by the coprecipitation method. Two varying Fe 3 O 4 /tetraethoxysilane ratios were used for silanization: 1:1.3 and 1:4.5. The samples were investigated using transmission electron microscopy, ferromagnetic resonance, IR spectroscopy, and magnetometry. Magnetic measurements have shown that the magnetite core in nanoparticles has a higher magnetization than stoichiometric magnetite nanoparticles of the same size. The increased magnetization was caused by the deviation of the magnetite stoichiometry due to the interaction with the silicate coating. The blocking temperature distribution was determined from the temperature dependence of the coercive force and from the ZFC/FC dependencies. Nanoparticles with a thicker shell have shown greater efficiency in DNA isolation.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…DNA isolation with 1:4.5 particles made it possible to isolate up to 3.6 times more DNA per 1 mg of sorbent, and the inhibitory effect was not observed in further studies. 30…”

Section: Resultsmentioning

confidence: 99%

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Manifestation of Stoichiometry Deviation in Silica-Coated Magnetite Nanoparticles

et al. 2022

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Starch-Coated Magnetic Iron Oxide Nanoparticles for Affinity Purification of Recombinant Proteins

Krasitskaya

Kudryavtsev

Yaroslavtsev

et al. 2022

IJMS

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Starch-coated magnetic iron oxide nanoparticles have been synthesized by a simple, fast, and cost-effective co-precipitation method with cornstarch as a stabilizing agent. The structural and magnetic characteristics of the synthesized material have been studied by transmission electron microscopy, Mössbauer spectroscopy, and vibrating sample magnetometry. The nature of bonds between ferrihydrite nanoparticles and a starch shell has been examined by Fourier transform infrared spectroscopy. The data on the magnetic response of the prepared composite particles have been obtained by magnetic measurements. The determined magnetic characteristics make the synthesized material a good candidate for use in magnetic separation. Starch-coated magnetic iron oxide nanoparticles have been tested as an affinity sorbent for one-step purification of several recombinant proteins (cardiac troponin I, survivin, and melanoma inhibitory activity protein) bearing the maltose-binding protein as an auxiliary fragment. It has been shown that, due to the highly specific binding of this fragment to the starch shell, the target fusion protein is selectively immobilized on magnetic nanoparticles and eluted with the maltose solution. The excellent efficiency of column-free purification, high binding capacity of the sorbent (100–500 µg of a recombinant protein per milligram of starch-coated magnetic iron oxide nanoparticles), and reusability of the obtained material have been demonstrated.

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Silica-Coated Iron Oxide Nanoparticles for DNA Isolation for Molecular Genetic Studies in Hematology

Cited by 2 publications

References 15 publications

Manifestation of Stoichiometry Deviation in Silica-Coated Magnetite Nanoparticles

Manifestation of Stoichiometry Deviation in Silica-Coated Magnetite Nanoparticles

Starch-Coated Magnetic Iron Oxide Nanoparticles for Affinity Purification of Recombinant Proteins

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