Starch-Coated Magnetic Iron Oxide Nanoparticles for Affinity Purification of Recombinant Proteins

Abstract

Starch-coated magnetic iron oxide nanoparticles have been synthesized by a simple, fast, and cost-effective co-precipitation method with cornstarch as a stabilizing agent. The structural and magnetic characteristics of the synthesized material have been studied by transmission electron microscopy, Mössbauer spectroscopy, and vibrating sample magnetometry. The nature of bonds between ferrihydrite nanoparticles and a starch shell has been examined by Fourier transform infrared spectroscopy. The data on the magnetic response of the prepared composite particles have been obtained by magnetic measurements. The determined magnetic characteristics make the synthesized material a good candidate for use in magnetic separation. Starch-coated magnetic iron oxide nanoparticles have been tested as an affinity sorbent for one-step purification of several recombinant proteins (cardiac troponin I, survivin, and melanoma inhibitory activity protein) bearing the maltose-binding protein as an auxiliary fragment. It has been shown that, due to the highly specific binding of this fragment to the starch shell, the target fusion protein is selectively immobilized on magnetic nanoparticles and eluted with the maltose solution. The excellent efficiency of column-free purification, high binding capacity of the sorbent (100–500 µg of a recombinant protein per milligram of starch-coated magnetic iron oxide nanoparticles), and reusability of the obtained material have been demonstrated.

Advances in superparamagnetic iron oxide nanoparticles modified with branched polyethyleneimine for multimodal imaging

“…Natural Materials Dextran Enables optimum polar interactions with iron oxide surfaces and improves blood circulation time, stability, and biocompatibility (Berry et al, 2003;Berry et al, 2004;Mikhaylova et al, 2004) Starch Improves biocompatibility and is good for MRI and drug target delivery (Jie et al, 2019;Krasitskaya et al, 2022) Gelatin Used as a gelling agent and hydrophilic emulsifier; biocompatible (Olsen et al, 2003;Gaihre et al, 2008) Chitosan Nontoxic, alkaline, and hydrophilic; widely used as nonviral gene delivery system; biocompatible and hydrophilic (Li et al, 2008a;Li et al, 2008b) Synthetic Polymers PEG Enhances hydrophilicity and water, solubility and improves biocompatibility and blood circulation times (Paul et al, 2004;Mondini et al, 2008) Poly (vinyl alcohol) (PVA) Prevents agglomeration, giving rise to monodispersibility (Pardoe et al, 2001;Chastellain et al, 2004;D'Souza et al, 2004) Poly (lactide acid) (PLA) Improves biocompatibility and biodegradability; low toxicity in the human body (Gomez-Lopera et al, 2006;Chena et al, 2008) Alginate Improves stability and biocompatibility (Ma et al, 2008;Morales et al, 2008) Polymethylmethacrylate (PMMA)…”

Increasing the Particle Size and Magnetic Property of Iron Oxide Nanoparticles through a Segregated Nucleation and Growth Process

“…For in vivo applications, magnetic nanoparticles can not only be used as markers to assist in the detection and diagnosis of clinical pathology [11,12], but also as a tool for loading certain drugs to achieve directional transport and release [13,14]. For in vitro applications, the surface of magnetic nanoparticles can be modified with a variety of ligands (or receptors), which can be specifically bound to the receptors (or ligands) for the detection and separation of specific cells, viruses, proteins, and nucleic acids under the action of an applied magnetic field [15][16][17][18].…”

Size-Dependent Spontaneous Separation of Colloidal Particles in Sub-Microliter Suspension by Cations

“…Separating and isolating micro/nanoparticles in suspension, especially in small-volume specimens, is a critical step in various environmental and biomedical applications [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. In order to handle precious and vital specimens of small volume, methods relying on precisely fabricated instruments and skilled operations have been developed over the last few decades, such as surface acoustic waves [ 14 , 15 , 16 ], magnetic control of paramagnetic/diamagnetic particles [ 17 , 18 , 19 , 20 , 21 ], dielectrophoresis [ 22 , 23 ] and microfluidic techniques [ 11 , 24 , 25 , 26 ]. However, separating small-volume specimens in an economical and widely applicable manner remains a great challenge [ 27 ], where a simple, maneuverable method with extremely low specimen consumption is the key prerequisite.…”