Silica Nanoparticles with Continuously Tunable Sizes: Synthesis and Size Effects on Cellular Contrast Imaging

Jin, Yuhui; Lohstreter, Samuel; Pierce, David T.; Parisien, Jenna; Wu, Min; Hall, Clifford; Zhao, Julia Xiaojun

doi:10.1021/cm8007478

Cited by 101 publications

(109 citation statements)

References 39 publications

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…This synthetic process takes more time than the Stöber method. However, the reverse microemulsion method can overcome the limitations of the Stöber method [25,[55][56][57] by producing silica nanoparticles with a uniform size distribution. Furthermore, this method can efficiently dope dye molecules into the silica nanoparticles.…”

Section: Reverse Microemulsion Methodsmentioning

confidence: 99%

Silica-Based Nanoparticles: Design and Properties

Liu

John

et al. 2010

Advanced Fluorescence Reporters in Chemistry and Biology II

Self Cite

View full text Add to dashboard Cite

A number of dye-doped silica nanoparticles (DDSNs) have been developed by encapsulating various fluorophores into silica nanomatrixes. These DDSNs have shown great potential as highly sensitive fluorescent labeling agents for biosensing and bioimaging. The silica nanomatrix affects the radiative properties and reactivity of doped fluorophores and endows the DDSN surface properties of silica. Compared with molecular fluorophores, the DDSNs exhibit advantageous properties such as high fluorescence intensity, good photostability, and biological compatibility. In this chapter, methods for silica nanoparticle synthesis and approaches for doping dye molecules within the silica nanomatrixes are overviewed. Various advantageous properties of DDSNs are discussed including reaction kinetics, solubility, photostability, quantum yield, lifetime, and toxicity.

show abstract

Section: Reverse Microemulsion Methodsmentioning

confidence: 99%

Silica-Based Nanoparticles: Design and Properties

Liu

John

et al. 2010

Advanced Fluorescence Reporters in Chemistry and Biology II

Self Cite

View full text Add to dashboard Cite

show abstract

“…Jin and co-workers studied the effect of size on the penetrating ability of NPs fabricated by ME and they showed that the small NPs (23 ± 3 nm) clearly penetrated the cells much faster than larger NPs (85 ± 5 nm). [ 41 ] In addition, the better image quality achieved with NPs-LA is assigned to their larger values of σ TPA (see Figure 6 ).…”

Section: Organic Fluorescent Nanoparticles For Bioimaging Applicationsmentioning

confidence: 97%

“…Size distribution Medium [27,28] Wide [32,38] Narrow [34] Tunable size Easy a) [15,36] Easy [39] Easy [40,41] Stability Stable fl uorescence a) [42] Stable fl uorescence [33] Stable fl uorescence [43,44] Aggregation Low [15,28,45] Low-medium [46] Medium [47][48][49] Surfactant Not necessary a) [50,51] Not necessary [31,52] Removed after purifi cation…”

Section: Structurementioning

confidence: 99%

Two‐Photon Imaging of a Cellular Line Using Organic Fluorescent Nanoparticles Synthesized by Laser Ablation

Aparicio-Ixta

Alba-Rosales

Ramos‐Ortíz

et al. 2016

Part & Part Syst Charact

View full text Add to dashboard Cite

A comparative study of the optical properties of organic fl uorescent nanoparticles fabricated by laser ablation (NPs-LA), reprecipitation (NPs-RP), and microemulsion (NPs-ME) methods is presented. These nanoparticles contain a fl uorene-based p-conjugated molecule (BT2). Distinctive electronic transitions are observed in samples due to the specifi c way in which the molecule BT2 is assembled in each type of nanoparticles; for instance, transitions involved in absorption and emission spectra of NPs-LA result in blueshifting with respect to the molecular solution of BT2, whereas redshifting is observed in NPs-RP and NPs-ME. Further, the results show that under infrared excitation, the aqueous suspensions of NPs-LA exhibit the highest fl uorescence induced by two-photon absorption (≈790 GM at 740 nm), as well as the best photostability, compared with aqueous suspensions of NPs-RP and NPs-ME. The nanoparticles synthetized by the three aforementioned methods are employed as exogenous agents for the visualization of human cervical cancer cell line (HeLa) using confocal and two-photon microscopy. Under similar experimental conditions, it is found that microscopy images of the best quality are obtained with NPs-LA. These results show that laser ablation is a suitable technique for the fabrication of organic fl uorescent nanoparticles used as contrast agents for in vitro fl uorescence microscopy.O-NPs have been successfully exploited as contrast agents (fl uorescent markers) for in vitro and in vivo studies in cellular lines and organisms, [ 5 ] medical imaging, [ 6 ] diagnostic purposes, [ 7 ] and positive confi rmation of the site-specifi c delivery of a drug with the goal of obtaining effective treatment of diseases. [ 8,9 ] Some advantages obtained by using organic nanostructures are: i) stability of fl uorescent organic materials in aqueous suspensions, ii) reduced toxicity, iii) decreased degradation, and iv) selectivity toward specifi c targets.Simple and versatile strategies of fabrication have been used to obtain O-NPs with different morphologies and optical properties in aqueous media. Reprecipitation (RP), [ 10 ] self-assembly, [ 11 ] ion-association, [ 12 ] condensation, [ 13 ] microemulsion (ME), [ 14 ] and laser ablation (LA) [ 15 ] are some methods used to fabricate O-NPs. For the preparation of O-NPs comprising luminescent properties, the most commonly employed methods are RP and ME. [ 16,17 ] LA has also been used to obtain fl uorescent O-NPs, [18][19][20] and some basic characterization of their optical properties has been performed, but to the best of our knowledge, demonstration of their use in the context of biophotonic applications, i.e., imaging of biological samples, has

show abstract

“…nanowires [9], nanotubes [10,11] and nanorods [12]) as well as 3D silica nanostructures (e.g. nanoparticles [13,14] and nanocubes [15]) have been widely investigated. Recently, graphene-supported large area silica nanosheets have been reported by our group [16].…”

mentioning

confidence: 99%

Graphene-templated approach to ultrathin silica nanosheets

2012

View full text Add to dashboard Cite

Graphene, a perfect two-dimensional (2D) nanostructure, is an ideal template for 2D material design. We developed a graphenetemplated method to synthesize 2D silica nanosheets through the crosslinking of poly(3-methacryloxypropyl trimethoxysilane)-grafted graphene oxide (GO-g-PMPS), followed by pyrolysis at 700°C for 10 h. graphene oxide (GO), poly(3-methacryloxypropyl trimethoxysilane) (PMPS), 2D silica nanosheets Citation:Kan L Y, Zheng B N, Gao C. Graphene-templated approach to ultrathin silica nanosheets.

show abstract

Silica Nanoparticles with Continuously Tunable Sizes: Synthesis and Size Effects on Cellular Contrast Imaging

Cited by 101 publications

References 39 publications

Silica-Based Nanoparticles: Design and Properties

Silica-Based Nanoparticles: Design and Properties

Two‐Photon Imaging of a Cellular Line Using Organic Fluorescent Nanoparticles Synthesized by Laser Ablation

Graphene-templated approach to ultrathin silica nanosheets

Contact Info

Product

Resources

About