Silica Release from Silane Cross-Linked Gelatin Based Hybrid Scaffold Affects Cell Proliferation

Balavigneswaran, Chelladurai Karthikeyan; Ramya, Venkatesan; Shyam, Karuppiah Prakash; Kumar, Gaurav; Paliwal, Pankaj; Krishnamurthy, Sairam; Kadalmani, Balamuthu; Mahto, Sanjeev Kumar; Misra, Nira

doi:10.1021/acsabm.9b00680

Cited by 19 publications

(19 citation statements)

References 52 publications

(93 reference statements)

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“…Gene expression was normalized to GAPDH. Fold change was calculated using the 2 –Δ Δ cq method as described earlier ( Balavigneswaran et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Dual Roles of Coconut Oil and Its Major Component Lauric Acid on Redox Nexus: Focus on Cytoprotection and Cancer Cell Death

et al. 2022

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It has been reported that coconut oil supplementation can reduce neuroinflammation. However, coconut oils are available as virgin coconut oil (VCO), crude coconut oil (ECO), and refined coconut oil (RCO). The impact of coconut oil extraction process (and its major fatty acid component lauric acid) at cellular antioxidant level, redox homeostasis and inflammation in neural cells is hitherto unexplained. Herein, we have shown the antioxidant levels and cellular effect of coconut oil extracted by various processes in human neuroblastoma cells (SH-SY5Y) cultured in vitro. Results indicate VCO and ECO treated cells displayed better mitochondrial health when compared to RCO. Similar trend was observed for the release of reactive oxygen species (ROS), key oxidative stress response genes (GCLC, HO-1, and Nqo1) and inflammatory genes (IL6, TNFα, and iNOS) in SH-SY5Y cells. Our results signified that both VCO and ECO offer better neural health primarily by maintaining the cellular redox balance. Further, RCO prepared by solvent extraction and chemical refining process lacks appreciable beneficial effect. Then, we extended our study to find out the reasons behind maintaining the cellular redox balance in neuroblastoma cells by VCO and ECO. Our GC-MS results showed that lauric acid (C14:0) (LA) content was the major difference in the fatty acid composition extracted by various processes. Therefore, we evaluated the efficacy of LA in SH-SY5Y cells. The LA showed dose-dependent effect. At IC50 concentration (11.8 μM), LA down regulated the oxidative stress response genes and inflammatory genes. The results clearly indicate that the LA inhibited the neuroinflammation and provided an efficient cellular antioxidant activity, which protects the cells. The efficiency was also evaluated in normal cell line such as fibroblasts (L929) to cross-validate that the results were not false positive. Different concentration of LA on L929 cells showed high compatibility. From our observation, we conclude that VCO and ECO offers better cellular protection owing to their powerful antioxidant system. Therefore, we advocate the inclusion of either VCO and/or ECO in the diet for a healthy lifestyle.

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“…Gene expression was normalized to GAPDH. Fold change was calculated using the 2 –Δ Δ cq method as described earlier ( Balavigneswaran et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Dual Roles of Coconut Oil and Its Major Component Lauric Acid on Redox Nexus: Focus on Cytoprotection and Cancer Cell Death

et al. 2022

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“…This kind of scaffolds is biocompatible, and the porosity and mechanical strength can be controlled by the inorganic–organic coupling for being scaffolds on tissue engineering [ 87 , 88 ]. Moreover, the chemical crosslinking increases the hydrophobicity and thermal stability of these scaffolds [ 89 ]. The GPTMS hydrolysis at highly acid pH (H 2 SO 4 aqueous solution) and oxidation with NaIO 4 transform the epoxy into an aldehyde functionality, which forms a Schiff base with lysine residues at pH higher 10.1 (pK of the Lys).…”

Section: Mechanisms Of Silica/protein and Silica/polysaccharide Interactions And Their Impacts On The Functional Properties Of The Derivementioning

confidence: 99%

“…is kind of scaffolds is biocompatible, and the porosity and mechanical strength can be controlled by the inorganic-organic coupling for being scaffolds on tissue engineering [87,88]. Moreover, the chemical crosslinking increases the hydrophobicity and thermal stability of these scaffolds [89].…”

Section: Functionalization Of Protein or Polysaccharide Hydrogel Precursor With Silanesmentioning

confidence: 99%

Silica/Protein and Silica/Polysaccharide Interactions and Their Contributions to the Functional Properties of Derived Hybrid Wound Dressing Hydrogels

Mesa

Becerra

2021

International Journal of Biomaterials

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Multifunctional and biocompatible hydrogels are on the focus of wound healing treatments. Protein and polysaccharides silica hybrids are interesting wound dressing alternatives. The objective of this review is to answer questions such as why silica for wound dressings reinforcement? What are the roles and contributions of silane precursors and silica on the functional properties of hydrogel wound dressings? The effects of tailoring the porous, morphological, and chemical characteristics of synthetic silicas on the bioactivity of hybrid wound dressings hydrogels are explored in the first part of the review. This is followed by a commented review of the mechanisms of silica/protein and silica/polysaccharide interactions and their impact on the barrier, scaffold, and delivery matrix functions of the derived hydrogels. Such information has important consequences for wound healing and paves the way to multidisciplinary researches on the production, processing, and biomedical application of this kind of hybrid materials.

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“…to Gel could be due to the hydrophilicity of the polymer, as observed in the earlier reports published by our group and other authors. [23,33,36] It has been reported that up to 10% hemolysis is permissible for biomaterials. [54] Thus 0.…”

Section: Effects Of Ss-plg On Interaction With Bsamentioning

confidence: 99%

“…[22] Crosslinking the gelatin-grafted polymer with silane increased the hydrophobicity as well as the stability of scaffolds. [23] Studies have revealed that gelatin notably inhibits the formation of insulin amyloid fibrils. [24] Therefore, it is important to elucidate the biocompatibility of gelatin grafted poly(D,L-lactide) (PDLLA) and its effect on amyloid fibril formation to understand the potential of the synthetic polymers as anti-amyloidogenic agents.…”

Section: Introductionmentioning

confidence: 99%

Gelatin grafted poly(D,L‐lactide) as an inhibitor of protein aggregation: An in vitro case study

Balavigneswaran

Kumar

Kumar³

et al. 2020

Biopolymers

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Amyloids are a group of proteins that are capable of forming aggregated amyloid fibrils, which is responsible for many neurodegenerative diseases including Alzheimer's disease (AD). In our previous study, synthesis and characterization of star‐shaped poly(D,L‐lactide)‐b‐gelatin (ss‐pLG) have been reported. In the present work, we have extended our work to study ss‐pLG against protein aggregation. To the best of our knowledge, this is the first report on the inhibition of amyloid fibrillation by protein grafted poly(D,L‐lactide). Bovine serum albumin (BSA) was chosen as the model protein, which readily forms fibril under high temperature. We found that ss‐pLG efficiently suppressed the fibril formation of BSA compared with gelatin (Gel), which was supported by Thioflavin T assay, circular dichroism (CD) spectroscopy and atomic force microscopy (AFM). In addition, ss‐pLG significantly curtailed amyloid‐induced hemolysis. We also found that incubation of ss‐pLG with neuroblastoma cells (MC65) protected the cells from fibril‐induced toxicity. The rescuing efficiency of ss‐pLG was better than Gel, which could be attributed to the reduced lamella thickness in branched ss‐pLG. These results suggest the significance of gelatin grafting, which probably allows gelatin to interact with the key residues of the amyloidogenic core of BSA effectively.

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Silica Release from Silane Cross-Linked Gelatin Based Hybrid Scaffold Affects Cell Proliferation

Cited by 19 publications

References 52 publications

Dual Roles of Coconut Oil and Its Major Component Lauric Acid on Redox Nexus: Focus on Cytoprotection and Cancer Cell Death

Dual Roles of Coconut Oil and Its Major Component Lauric Acid on Redox Nexus: Focus on Cytoprotection and Cancer Cell Death

Silica/Protein and Silica/Polysaccharide Interactions and Their Contributions to the Functional Properties of Derived Hybrid Wound Dressing Hydrogels

Gelatin grafted poly(D,L‐lactide) as an inhibitor of protein aggregation: An in vitro case study

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