Silicate-based bioceramic scaffolds for dual-lineage regeneration of osteochondral defect

Bunpetch, Varitsara; Zhang, Xiaoan; Tian, Li; Lin, Junxin; Maswikiti, Ewetse Paul; Wu, Yan; Cai, Dandan; Li, Jun; Zhang, Shufang; Wu, Chengtie; Ouyang, Hongwei

doi:10.1016/j.biomaterials.2018.11.025

Cited by 119 publications

(75 citation statements)

References 54 publications

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“…Furthermore, in the case of laminate composites, the weak strength bonding between layers normally results in phase separation, not leading to tissue regeneration. Therefore, some reports focused on the use of single scaffolds for osteochondral regeneration [15,16]. These scaffolds ensure continuity between phases to avoid a barrier in the interface, together with biomaterials integrity, while mimicking the natural hierarchical structure of the osteochondral tissue.…”

Section: A Multifactorial Toolbox For Designing Tissue Engineering Stmentioning

confidence: 99%

A Physiology-Inspired Multifactorial Toolbox in Soft-to-Hard Musculoskeletal Interface Tissue Engineering

Calejo

Costa‐Almeida

Reis

et al. 2020

Trends in Biotechnology

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Section: A Multifactorial Toolbox For Designing Tissue Engineering Stmentioning

confidence: 99%

A Physiology-Inspired Multifactorial Toolbox in Soft-to-Hard Musculoskeletal Interface Tissue Engineering

Calejo

Costa‐Almeida

Reis

et al. 2020

Trends in Biotechnology

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“…Silicon is a kind of trace element in the human body and is especially important for bone growth and development [31]. Extensive studies have confirmed that silicon-based biomaterials can not only promote osteogenesis formation but also stimulate angiogenesis via BMSC interaction [4, 32]. In recent years, an increasing number of researchers have paid attention to the cytotoxic mechanisms of silicon-doped materials; whether silicon nanomaterials or high concentration of free silicate, both can affect the cell viability to some extent [33].…”

Section: Discussionmentioning

confidence: 99%

Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells

Liu

et al. 2019

Stem Cell Res Ther

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BackgroundSilicon-modified biomaterials have been extensively studied in bone tissue engineering. In recent years, the toxicity of silicon-doped biomaterials has gradually attracted attention but requires further elucidation. This study was designed to explore whether high-dose silicate can induce a cytotoxicity effect in bone mesenchymal stem cells (BMSCs) and the role of autophagy in its cytotoxicity and mechanism.MethodsMorphologic changes and cell viability of BMSCs were detected after different doses of silicate exposure. Autophagic proteins (LC3, p62), LC3 turnover assay, and RFP-GFP-LC3 assay were applied to detect the changes of autophagic flux following silicate treatment. Furthermore, to identify the potential mechanism of autophagic dysfunction, we tested the acetyl-α-tubulin protein level and histone deacetylase 6 (HDAC6) activity after high-dose silicate exposure as well as the changes in microtubule and autophagic activity after HDAC6 siRNA was applied.ResultsIt was found that a high dose of silicate could induce a decrease in cell viability; LC3-II and p62 simultaneously increased after high-dose silicate exposure. A high concentration of silicate could induce autophagic dysfunction and cause autophagosomes to accumulate via microtubule destabilization. Results showed that acetyl-α-tubulin decreased significantly with high-dose silicate treatment, and inhibition of HDAC6 activity can restore microtubule structure and autophagic flux.ConclusionsMicrotubule destabilization caused by a high concentration of silicate via HDAC6 activation contributed to autophagic dysfunction in BMSCs, and inhibition of HDAC6 exerted a cytoprotection effect through restoration of the microtubule structure and autophagic flux.

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“…In a typical bone regeneration process, scaffolds placed in bone defect sites can lead to rapid initiation of efficient cell recruitment, adhesion, proliferation, and osteodifferentiation, which are important for bone regeneration and bone remodeling. 54,55 Eight weeks after treatment, the tibia plateau of the VCM-NPs/Gel group appeared flatter than that of the VCM/Gel group. The images of the 3D reconstruction in Figure 8C indicate a progressive increase in bone volume at the end of the 8th week post-treatment with VCM/Gel, VCM-NPs/Gel, and VCS, while it tended toward bone loss in the model groups.…”

Section: Radiographic Datamentioning

confidence: 98%

<p>Injectable Chitosan-Based Thermosensitive Hydrogel/Nanoparticle-Loaded System for Local Delivery of Vancomycin in the Treatment of Osteomyelitis</p>

Jin¹,

Zhang²,

Shen³

et al. 2020

IJN

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Purpose: Osteomyelitis, particularly chronic osteomyelitis, remains a major challenge for orthopedic surgeons. The traditional treatment for osteomyelitis, which involves antibiotics and debridement, does not provide a complete solution for infection and bone repair. Antibiotics such as vancomycin (VCM) are commonly used to treat osteomyelitis in clinical settings. VCM use is limited by a lack of effective delivery methods that provide sustained, high doses to entirely fill irregular bone tissue to treat infections. Methods: We engineered a chitosan (CS)-based thermosensitive hydrogel to produce a VCM-nanoparticle (NPs)/Gel local drug delivery system. The VCM-NPs were formed with quaternary ammonium chitosan and carboxylated chitosan nanoparticles (VCM-NPs) by positive and negative charge adsorption to enhance the encapsulation efficiency and drug loading of VCM, with the aim of simultaneously preventing infection and repairing broken bones. This hydrogel was evaluated in a rabbit osteomyelitis model. Results: The VCM-NPs had high encapsulation efficiency and drug loading, with values of 60.1±2.1% and 24.1±0.84%, respectively. When embedded in CS-Gel, the VCM-NPs maintained their particle size and morphology, and the injectability and thermosensitivity of the hydrogel, which were evaluated by injectability test and rheological measurement, were retained. The VCM-NPs/Gel exhibited sustained release of VCM over 26 days. In vitro tests revealed that the VCM-NPs/Gel promoted osteoblast proliferation and activity against Staphylococcus aureus. In vivo, VCM-NPs/Gel (with 10 mg vancomycin per rabbit) was used to treat rabbits with osteomyelitis. The VCM-NPs/Gel showed excellent anti-infection properties and accelerating bone repair under osteomyelitis conditions. Conclusion: The reported multifunctional NPs hydrogel system for local antibiotic delivery (VCM-NPs/Gel) showed bone regeneration promotion and anti-infection properties, demonstrating significant potential as a scaffold for effective treatment of osteomyelitis.

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Silicate-based bioceramic scaffolds for dual-lineage regeneration of osteochondral defect

Cited by 119 publications

References 54 publications

A Physiology-Inspired Multifactorial Toolbox in Soft-to-Hard Musculoskeletal Interface Tissue Engineering

A Physiology-Inspired Multifactorial Toolbox in Soft-to-Hard Musculoskeletal Interface Tissue Engineering

Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells

<p>Injectable Chitosan-Based Thermosensitive Hydrogel/Nanoparticle-Loaded System for Local Delivery of Vancomycin in the Treatment of Osteomyelitis</p>

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