Silicate bioceramics enhanced vascularization and osteogenesis through stimulating interactions between endothelia cells and bone marrow stromal cells

Li, Haiyan; Xue, Ke; Kong, Ni; Liu, Kai; Chang, Jiang

doi:10.1016/j.biomaterials.2014.01.039

Cited by 230 publications

(177 citation statements)

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“…However, the osteoblast differentiation master transcriptional factors were not significantly induced within this short period, although alkaline phosphatase tissue-nonspecific isoform (ALPL) was overexpressed ( Fig. 6B and Supplementary Table S17), which was consistent with other literatures [12,13,26].…”

Section: Et Alsupporting

confidence: 90%

“…Tissue engineering-based research in both two-dimensional (2D) and three-dimensional cocultures in vitro revealed that the active interaction between ECs and MSCs (or osteoprogenitor and fibroblast) induced the formation of tube-like cell aggregation structures. This result indicates that angiogenesis and prevascularization are involved in the cellcell communication and remodeling of the functional blood vessel development in vivo [5,[18][19][20][21][22][23][24][25][26]. Moreover, MSCs could repress ECs by regulating cytokine-induced leukocyte recruitment [27], while the activated ECs could regulate the MSCs-to-ECs transmigration in a leukocyte-like mechanism [28,29].…”

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confidence: 99%

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Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

LiJunxiang

MaYing

TengRuifang

et al. 2015

Stem Cells and Development

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Mesenchymal stem cells (MSCs) show great promise in blood vessel restoration and vascularization enhancement in many therapeutic situations. Typically, the co-implantation of MSCs with vascular endothelial cells (ECs) is effective for the induction of functional vascularization in vivo, indicating its potential applications in regenerative medicine. The effects of MSCs-ECs-induced vascularization can be modeled in vitro, providing simplified models for understanding their underlying communication. In this article, a contact coculture model in vitro and an RNA-seq approach were employed to reveal the active crosstalk between MSCs and ECs within a short time period at both morphological and transcriptional levels. The RNA-seq results suggested that angiogenic genes were significantly induced upon coculture, and this prevascularization commitment might require the NF-kB signaling. NF-kB blocking and interleukin (IL) neutralization experiments demonstrated that MSCs potentially secreted IL factors including IL1b and IL6 to modulate NF-kB signaling and downstream chemokines during coculture. Conversely, RNA-seq results indicated that the MSCs were regulated by the coculture environment to a smooth muscle commitment within this short period, which largely induced myocardin, the myogenic co-transcriptional factor. These findings demonstrate the mutual molecular mechanism of MSCs-ECs-induced prevascularization commitment in a quick response.

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Section: Et Alsupporting

confidence: 90%

mentioning

confidence: 99%

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

LiJunxiang

MaYing

TengRuifang

et al. 2015

Stem Cells and Development

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“…31,32 The ionic dissolution products used in the present work have insufficient concentration of Si given that they were obtained using HBSS containing glucose (1 g L À1 ), an agent known to delay the dissolution of BGs. 33 However, our preliminary degradation studies in HBSS for 72 h revealed that Si concentrations leached from particles (<5 mm) of 45S5 BG and 45S5.2B BG were 18.79 mg mL À1 and 17.82 mg mL À1 , respectively, while the amount of borate ions released from the 45S5.2B BG particles was about 20 mM.…”

Section: Discussionmentioning

confidence: 99%

Angiogenic effects of ionic dissolution products released from a boron-doped 45S5 bioactive glass

et al. 2015

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In regenerative medicine of vascularized tissues, there is a great interest in the use of biomaterials that are able to stimulate angiogenesis, a process necessary for rapid revascularization to allow the transport and exchange of oxygen, nutrients, growth factors and cells that take part in tissue repair and/or regeneration. An increasing number of publications have shown that bioactive glasses stimulate angiogenesis. Because it has been established that boron (B) may play a role in angiogenesis, the aim of this study was to assess the in vivo angiogenic effects of the ionic dissolution products that from a bioactive glass (BG) in the 45S5 system doped with 2 wt% B 2 O 3 (45S5.2B). The pro-angiogenic capacity of 45S5.2B BG was assessed on the vasculature of the embryonic quail chorioallantoic membrane (CAM).Ionic dissolution products from 45S5.2B BG increased angiogenesis. This is quantitatively evidenced by the greater expression of integrin a v b 3 and higher vascular density in the embryonic quail CAM. The response observed at 2 and 5 days post-treatment was equivalent to that achieved by applying 10 mg mL À1 of basic fibroblast growth factor. These results show that the ionic dissolution products released from the bioactive glass 45S5.2B stimulate angiogenesis in vivo. The effects observed are attributed to the presence the ionic dissolution products, which contained 160 AE 10 mM borate.

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“…One of the authors' labs has demonstrated that silicate-based biomaterials stimulate osteogenesis and angiogenesis. The Si/Ca ions released from the materials stimulate osteogenic differentiation of bone marrow mesenchymal 7,56 or urine-derived stem cells (USCs) 57 and angiogenic differentiation of endothelial cells (ECs) when SC or EC are separately cultured 7,57 or in a SC-EC co-culture 56 system. Here we report that hESCs tend to differentiate into mesodermal cells in the presence of CS extracts (Fig.…”

Section: Discussionmentioning

confidence: 99%

Bioactive calcium silicate extracts regulate the morphology and stemness of human embryonic stem cells at the initial stage

Zhang

Lü

et al. 2016

RSC Adv.

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Embryonic stem cells (ESCs) are undifferentiated cells that have the capacity to self-renew and differentiate into a variety of cells and provide cell sources for regenerative medicine or biological specimens for cytotoxicity tests. Calcium silicate (CS), a bioactive silicate ceramic, can stimulate the osteogenic differentiation of various types of stem cells, but its role in regulating the biological phenotypes of ESCs remains unclear. Here, the impact of CS on human ESCs was investigated using CS-supplemented medium. The cytotoxicity of CS to hESCs and its effects on apoptosis, growth, proliferation, and differentiation were quantified systematically. Morphological analysis of hESC colonies indicated that the bioactive ions released from CS have little cytotoxicity to hESCs at two CS concentrations. Immunofluorescence and flow cytometry analyses showed that apoptosis was time-independent at early or late stages of hESC growth. In contrast, CS ion extracts regulated hESC differentiation in a time-dependent manner: ESC stemness was preserved by enhancing Oct-4, Sox-2, and Nanog gene expression at day 3, while the cells tended to differentiate at day 6. Combined tests on gene and protein levels further indicated that hESCs tended to differentiate into mesoderm in the presence of CS ion extracts, especially at low CS concentrations. These results demonstrate the effects of CS extracts on hESC stemness and differentiation at the molecular and cellular levels, suggesting that CS-based biomaterials could serve as a potential regulator for ESCs in regenerative medicine.

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Silicate bioceramics enhanced vascularization and osteogenesis through stimulating interactions between endothelia cells and bone marrow stromal cells

Cited by 230 publications

References 50 publications

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

Angiogenic effects of ionic dissolution products released from a boron-doped 45S5 bioactive glass

Bioactive calcium silicate extracts regulate the morphology and stemness of human embryonic stem cells at the initial stage

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