Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation

Bogdanov, E A; Доминова, И. Н.; Shusharina, Natalia; Botman, Stepan; Kasymov, Vitaliy; Патрушев, М. В.

doi:10.1371/journal.pone.0068280

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Titanium dioxide films were deposited on a commercially available pure titanium substrate, a nylon substrate, a PTFE substrate, and a monocrystalline silicon substrate by IBAD under argon gas flow. The ion beam deposition system has been described previously [22]. It mainly consisted of one Kaufman ion source and one Hall ion source, one target holder, and one rotatable planet-type sample holder in the path of Ar ion source and deposited material.…”

Section: Sample Preparationmentioning

confidence: 99%

The Particles Precipitation and Osseointegration of a Tio2 Thin-Film Coating by Ion Beam Deposition - An In vivo Study

Bogdanov¹,

Vavilina²,

Shusharina³

et al. 2014

J Nanomed Nanotechnol

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Figure 1: a) The RBS spectrum of TiO 2 deposited on monocrystalline silicon. b) AES wide scan spectra of TiO 2 thin film surface on PTFE substrate. c) AES wide scan spectra of TiO 2 thin film surface on PTFE substrate after ion etch. d) AES in-depth profiling of TiO 2 thin film surface on nylon substrate. Observed thin film without any impurities e) AES wide scan spectra of Ag thin film indicator layer on nylon implant model coated by TiO 2 thin film. f) SEM image of titanium implant for particles precipitation analysis. Citation: Bogdanov E, Vavilina Y, Shusharina N, Goykhman A, Patrushev M (2014) The Particles Precipitation and Osseointegration of a Tio 2 Thin-Film Coating by Ion Beam Deposition -An In vivo Study.

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Section: Sample Preparationmentioning

confidence: 99%

The Particles Precipitation and Osseointegration of a Tio2 Thin-Film Coating by Ion Beam Deposition - An In vivo Study

Bogdanov¹,

Vavilina²,

Shusharina³

et al. 2014

J Nanomed Nanotechnol

Self Cite

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Research progress of All-in-One PCR tube biosensors based on functional modification and intelligent fabrication

Wang,

Zhu,

Guo

et al. 2024

Biosensors and Bioelectronics

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DNA extraction from plant food supplements: Influence of different pharmaceutical excipients

Costa

Amaral

Fernandes

et al. 2015

Molecular and Cellular Probes

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a b s t r a c tThe consumption of plant food supplements (PFS) has been growing globally, with an increase of misleading labeling and fraudulent practices also being reported. Recently, the use of molecular biology techniques has been proposed to detect botanical adulterations, one of the possible frauds in PFS. However, difficulties in recovering DNA from some PFS samples have been described. Aiming at using DNA-based methods for the unequivocal identification of plant species in PFS, adequate DNA isolation is required. However, PFS often contain pharmaceutical excipients known to have adsorbent properties that might interfere with DNA extraction. Thus, the aim of this work was to assess the effect of different excipients (talc, silica, iron oxide and titanium dioxide) on the recovery/amplification of DNA. For that purpose, known amounts of template maize DNA were spiked either to PFS or to model mixtures of excipients and quantified by real-time PCR. The tested excipients evidenced clear adsorption phenomena that justify the hampering effect on DNA extraction from PFS. The use of either 10% talc or 0.5% dyes completely adsorbed DNA, resulting in negative PCR amplifications. For the first time, pharmaceutical excipients were shown to affect DNA extraction explaining the inability of recovering DNA from some PFS samples in previous studies.

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Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation

Cited by 3 publications

References 22 publications

The Particles Precipitation and Osseointegration of a Tio2 Thin-Film Coating by Ion Beam Deposition - An In vivo Study

The Particles Precipitation and Osseointegration of a Tio2 Thin-Film Coating by Ion Beam Deposition - An In vivo Study

Research progress of All-in-One PCR tube biosensors based on functional modification and intelligent fabrication

DNA extraction from plant food supplements: Influence of different pharmaceutical excipients

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