Silk fibroin and sodium alginate blend: Miscibility and physical characteristics

Moraes, Mariana Agostini de; Silva, Mariana Ferreira; Weska, Raquel Farias; Beppu, Marisa Masumi

doi:10.1016/j.msec.2014.03.047

Cited by 40 publications

(25 citation statements)

References 27 publications

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“…So far, there have been studies on beneficial properties of SF/SA blended films and hydrogels. There was a study reporting SF/SA blend films with globular micro-structure exhibited suitable water vapor transmission, swelling capacity, mechanical properties and non-cytotoxity for wound dressings32. In other case studies, the SF/SA hydrogel scaffolds crosslinked by Ca 2+ were used for recreating artificial stem cell niche33, and another novel SF/SA hybrid scaffolds offered new and important options to the needs related to biomineralization in tissue engineering34.…”

mentioning

confidence: 99%

A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering

Wang

Shi

et al. 2016

Sci Rep

156

103

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A cytocompatible porous scaffold mimicking the properties of extracellular matrices (ECMs) has great potential in promoting cellular attachment and proliferation for tissue regeneration. A biomimetic scaffold was prepared using silk fibroin (SF)/sodium alginate (SA) in which regular and uniform pore morphology can be formed through a facile freeze-dried method. The scanning electron microscopy (SEM) studies showed the presence of interconnected pores, mostly spread over the entire scaffold with pore diameter around 54~532 μm and porosity 66~94%. With significantly better water stability and high swelling ratios, the blend scaffolds crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) provided sufficient time for the formation of neo-tissue and ECMs during tissue regeneration. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) results confirmed random coil structure and silk I conformation were maintained in the blend scaffolds. What’s more, FI-TR spectra demonstrated crosslinking reactions occurred actually among EDC, SF and SA macromolecules, which kept integrity of the scaffolds under physiological environment. The suitable pore structure and improved equilibrium swelling capacity of this scaffold could imitate biochemical cues of natural skin ECMs for guiding spatial organization and proliferation of cells in vitro, indicating its potential candidate material for soft tissue engineering.

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mentioning

confidence: 99%

A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering

Wang

Shi

et al. 2016

Sci Rep

156

103

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“…In those studies, phase separation is observed, as well as the presence of globular structures even in low concentration of polymers (Antonov and Moldenaers, 2011;Mession et al, 2012). These structures are also observed by scanning electron microscopy in our previous study of SF-SA blends (de Moraes et al, 2014;Lopes et al, 2018), which investigated the physical-chemical behavior of the blends.…”

Section: Introductionmentioning

confidence: 55%

“…Silk fibroin solution was prepared following the method previously published (de Moraes et al, 2014). Briefly, Bombyx mori silkworm cocoons (Bratac, Brazil) were degummed using 1 g.L −1 Na 2 CO 3 solution in a thermostatic bath at 85 • C (Nova Analitica, Brazil) for 30 min, the process was repeated other two times.…”

Section: Preparation Of Solutions: Silk Fibroin and Sodium Alginatementioning

confidence: 99%

“…Blends of Silk fibroin (SF) and sodium alginate (SA) have been studied since the beginning of the 90’s years ( Liang and Hirabayashi, 1992 ). Most of the works with these two polymers explore the potential of the blends as a biomaterial due to their characteristics such as biocompatibility, biodegradability and low toxicity ( de Moraes and Beppu, 2013 ; de Moraes et al, 2014 ; Ming and Zuo, 2014 ; Zhang et al, 2015 ; Wang et al, 2016 ). Despite that, the commercialization of these products is not a reality due to the complexity of the system formed by these polymers.…”

Section: Introductionmentioning

confidence: 99%

“…So, macromolecules that interact through strong bonds, for example, hydrogen bonds, can form partially miscible systems. The interaction between SF and natural polysaccharides is well established in the literature and it can occur by hydrogen bonds and covalent bonds ( de Moraes et al, 2014 ; Zhang et al, 2015 ; Wang et al, 2019 ). In protein-polysaccharide aqueous systems, intermolecular complexes are formed and macroscopically phase separation occurs by thermodynamic incompatibility ( Mession et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phase Diagram and Estimation of Flory-Huggins Parameter of Interaction of Silk Fibroin/Sodium Alginate Blends

Lopes

Moraes

Beppu

2020

Front. Bioeng. Biotechnol.

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Silk fibroin (SF) and sodium alginate (SA) are natural polymers used to produce biomaterials. One of the strategies to improve the properties of these products is to prepare blends with them, which are partially miscible. Phase separation is observed, therefore, the thermodynamic analysis of this system is important to predict the final state and composition of this blends. This study explored blends with a different initial composition of SF, SA, and water (WA) at 25 • C and neutral pH. After phase separation, two phases were identified, one rich in SF and other rich in SA. The Flory-Huggins parameters of interaction of polymer-solvent and polymer-polymer were estimated using the extended equation and data of phase equilibrium, their values indicates the partial miscibility of the polymers.

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Alginate‐based Films and Membranes: Preparation, Characterization and Applications

2016

Nanostructured Polymer Membranes

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Silk fibroin and sodium alginate blend: Miscibility and physical characteristics

Cited by 40 publications

References 27 publications

A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering

A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering

Phase Diagram and Estimation of Flory-Huggins Parameter of Interaction of Silk Fibroin/Sodium Alginate Blends

Alginate‐based Films and Membranes: Preparation, Characterization and Applications

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