Silk Fibroin-Based Biomaterials in Biomedical Applications

Byram, Prasanna Kumar; Das, Lopamudra; Sunka, Krishna Chaitanya; Kulkarni, Gaurav; Dhara, Santanu; Chakravorty, Nishant

doi:10.1007/978-981-16-7152-4_8

Cited by 4 publications

(2 citation statements)

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“…has been a topic of intense research in recent years [23]. While both the polymers, SF and SS are biocompatible individually and have been used in various biomedical applications [25][26][27][28]; however, SS and SF in native form have been found to be pro-inflammatory [29]. This could be attributed to the presence of other biomolecules found in native silk, such as fibrohexamerin and seroin, which could be the cause of the inflammation [30].…”

Section: Introductionmentioning

confidence: 99%

Bioactive self-assembling silk fibroin–sericin films for skin tissue engineering

Byram,

Mukherjee,

Rahaman

et al. 2024

Biomed. Mater.

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The quest for an ideal wound dressing material has been a strong motivation for researchers to explore novel biomaterials for this purpose. Such explorations have led to the extensive use of silk fibroin as a suitable polymer for several applications over the years. Unfortunately, another major silk protein - sericin has not received its due attention yet in spite of having favorable biological properties. In this study, we report an approach of blending silk fibroin (SF) and silk sericin (SS) without the usage of chemical crosslinkers is made possible by the usage of formic acid (FA) which evaporates to induce β-sheets formation to form cytocompatible films. Raman spectroscopy confirms the presence of SF/SS components in blend and formation of β-sheet in films. In situ, gelation kinetics studies were conducted to understand the change in gelation properties with addition of sericin into silk fibroin. MTT and Live/dead assays were performed to study cellular attachment, viability and proliferation on SF/SS films. The antibacterial properties of SF/SS films were tested using Gram-negative and Gram-positive bacteria. The re-structured SF/SS films were stable, transparent, show good mechanical properties, antibacterial activity and cytocompatibility, therefore can serve as suitable biomaterial candidates for skin regeneration applications.

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Section: Introductionmentioning

confidence: 99%

Bioactive self-assembling silk fibroin–sericin films for skin tissue engineering

Byram,

Mukherjee,

Rahaman

et al. 2024

Biomed. Mater.

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“…Silk fibroin, a natural protein extracted from Bombyx mori cocoons, is endowed with outstanding mechanical properties, biocompatibility, and bioresorbability and has attracted significant attention as a biomedical material [28][29][30][31], including as a scaffold for tissue engineering [32][33][34][35]. The lysine residues present in silk fibroin provide free amino groups capable of participating in the aza-Michael polyaddition process leading to PAAs.…”

Section: Introductionmentioning

confidence: 99%

Toughening Polyamidoamine Hydrogels through Covalent Grafting of Short Silk Fibers

et al. 2022

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Linear amphoteric polyamidoamines (PAAs) are usually water-soluble, biodegradable and biocompatible. Crosslinked PAAs form in water hydrogels, retaining most of the favorable properties of their linear counterparts. The hydrogels prepared by the radical post-polymerization of the oligo-α,ω-bisacrylamido-terminated PAA called AGMA1, obtained by the polyaddition of 4-aminobutylguanidine (agmatine) with 2,2-bis(acrylamido)acetic acid, exhibit excellent cell-adhesion properties both in vitro and in vivo. However, due to their low mechanical strength, AGMA1 hydrogels cannot be sewn to biological tissues and need to be reinforced with fibrous materials. In this work, short silk fibers gave excellent results in this sense, proving capable of establishing covalent bonds with the PAA matrix, thanks to their lysine content, which provided amino groups capable of reacting with the terminal acrylamide groups of the AGMA1 precursor in the final crosslinking phase. Morphological analyses demonstrated that the AGMA1 matrix was intimately interconnected and adherent to the silk fibers, with neither visible holes nor empty volumes. The silk/H-AGMA1 composites were still reversibly swellable in water. In the swollen state, they could be sewn and showed no detachment between fibers and matrix and exhibited significantly improved mechanical properties compared with the plain hydrogels, particularly as regards their Young’s modulus and elongation at break.

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