Silk fibroin/carboxymethyl chitosan hydrogel with tunable biomechanical properties has application potential as cartilage scaffold

Li, Tao; Song, Xiongbo; Weng, Changmei; Wang, Xin; Gu, Liling; Gong, Xiaoyuan; Wei, Quanfang; Duan, Xiaojun; Yang, Liu; Chen, Cheng

doi:10.1016/j.ijbiomac.2019.06.245

Cited by 73 publications

(18 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, SF-1 hydrogel exhibited an average G’ of 140 Pa, and the G’ increased to 1200 Pa by increasing the concentration to 4%. The results show that the SF hydrogels are weak in strength, which is consistent with the previous studies reporting the low mechanical strength of SF hydrogel identified by enzyme-mediated crosslinking [ 29 , 30 ]. A recent study reported a G’ of less than 100 Pa for 1% SF hydrogel HRP-mediated crosslinking, which increased to 750 Pa by increasing the SF concentration to 6%, demonstrating the low mechanical stability of SF hydrogel [ 23 ].…”

Section: Resultssupporting

confidence: 91%

Ovarian Cell Encapsulation in an Enzymatically Crosslinked Silk-Based Hydrogel with Tunable Mechanical Properties

Jafari

Dadashzadeh

Moghassemi

et al. 2021

Gels

View full text Add to dashboard Cite

An artificial ovary is a promising approach for preserving fertility in prepubertal girls and women who cannot undergo current cryopreservation strategies. However, this approach is in its infancy, due to the possible challenges of creating a suitable 3D matrix for encapsulating ovarian follicles and stromal cells. To maintain the ovarian stromal cell viability and proliferation, as a first step towards developing an artificial ovary, in this study, a double network hydrogel with a high water swelling capacity (swelling index 15–19) was developed, based on phenol conjugated chitosan (Cs-Ph) and silk fibroin (SF) through an enzymatic crosslinking method using horseradish peroxidase. The addition of SF (1%) to Cs (1%) decreased the storage modulus (G’) from 3500 Pa (Cs1) to 1600 Pa (Cs-SF1), and the hydrogels with a rapid gelation kinetic produced a spatially homogeneous distribution of ovarian cells that demonstrated 167% proliferation after 7 days. This new Cs-SF hydrogel benefits from the toughness and flexibility of SF, and phenolic chemistry could provide the potential microstructure for encapsulating human ovarian stromal cells.

show abstract

Section: Resultssupporting

confidence: 91%

Ovarian Cell Encapsulation in an Enzymatically Crosslinked Silk-Based Hydrogel with Tunable Mechanical Properties

Jafari

Dadashzadeh

Moghassemi

et al. 2021

Gels

View full text Add to dashboard Cite

show abstract

“…Hydrogels are one of the smart materials in cartilage scaffolds due to their innate hydrated structure, distinctive biocompatibility and capacity to join chemical cues [138]. These benefits promote the development of ECM-like matrices derived from molecular building-blocks, such as elastin-like polymers [139], peptide amphiphiles [140] or the commercially available Puramatrix (3DM) [141], that imitate the function and structure of native ECM.…”

Section: The Next Generation Biomaterials For Cartilage Tissue Enginementioning

confidence: 99%

The Use of Nanomaterials in Tissue Engineering for Cartilage Regeneration; Current Approaches and Future Perspectives

Eftekhari

Dizaj

Sharifi

et al. 2020

IJMS

107

View full text Add to dashboard Cite

The repair and regeneration of articular cartilage represent important challenges for orthopedic investigators and surgeons worldwide due to its avascular, aneural structure, cellular arrangement, and dense extracellular structure. Although abundant efforts have been paid to provide tissue-engineered grafts, the use of therapeutically cell-based options for repairing cartilage remains unsolved in the clinic. Merging a clinical perspective with recent progress in nanotechnology can be helpful for developing efficient cartilage replacements. Nanomaterials, < 100 nm structural elements, can control different properties of materials by collecting them at nanometric sizes. The integration of nanomaterials holds promise in developing scaffolds that better simulate the extracellular matrix (ECM) environment of cartilage to enhance the interaction of scaffold with the cells and improve the functionality of the engineered-tissue construct. This technology not only can be used for the healing of focal defects but can also be used for extensive osteoarthritic degenerative alterations in the joint. In this review paper, we will emphasize the recent investigations of articular cartilage repair/regeneration via biomaterials. Also, the application of novel technologies and materials is discussed.

show abstract

“…This increase could be due to the presence of the microparticles in the hydrogel (Atoufi et al, 2019). In the year 2017, Li and his colleagues obtained less modulus of chondroitin sulfate/pullulan in cartilage tissue engineering (Li et al, 2018), also, in the year 2019, Li and his colleagues achieved similar results (on average in different compounds) on fibroin/CMC (Li et al, 2019). In fact, as the material changes, the pressure modulus changes.…”

Section: Resultsmentioning

confidence: 91%

Cartilage regeneration with dual‐drug‐releasing injectable hydrogel/microparticle system: In vitro and in vivo study

Naghizadeh

Karkhaneh

Nokhbatolfoghahaei

et al. 2020

Journal Cellular Physiology

View full text Add to dashboard Cite

In this study, we developed an injectable in situ forming hydrogel/microparticle system consisting of two drugs, melatonin and methylprednisolone, to investigate the capability of the system for chondrogenesis in vitro and in vivo. The chemical, mechanical, and rheological properties of the hydrogel/microparticle were investigated. For in vitro evaluation, the adipose-derived stem cells might be mixed with hydrogel/microparticles, then cellular viability was analyzed by acridine orange/propidium iodide and 4′,6-diamidino-2-phenylindole staining and also dimethylmethylene blue assay were conducted to find the amount of proteoglycan. The real-time polymerase chain reaction for aggrecan, sex-determining region Y-Box 9, collagen I (COL1), and COL2 gene expression was performed after 14 and 21 days. For evaluation of cartilage regeneration, the samples were implanted in rabbit knees with cartilaginous experimental defects. Defects were created in both knees of three groups of rabbits. Group 1 was the control with no injection, and Groups 2 and 3 were loaded with hydrogel/cell and hydrogel/microparticle/cell; respectively. Then, after 3 and 6 months, histological evaluations of the defected sites were carried out. The amount of glycosaminoglycans after 14 and 21 days increased significantly in hydrogels/microparticles loaded with cells. The expression of marker genes was also significant in hydrogels/microparticles loaded with cells. According to histology analysis, the hydrogels/microparticles loaded with cells showed the best cartilage regeneration. Overall, our study revealed that the developed injectable hydrogel/microparticle can be used for cartilage regeneration.

show abstract

Silk fibroin/carboxymethyl chitosan hydrogel with tunable biomechanical properties has application potential as cartilage scaffold

Cited by 73 publications

References 44 publications

Ovarian Cell Encapsulation in an Enzymatically Crosslinked Silk-Based Hydrogel with Tunable Mechanical Properties

Ovarian Cell Encapsulation in an Enzymatically Crosslinked Silk-Based Hydrogel with Tunable Mechanical Properties

The Use of Nanomaterials in Tissue Engineering for Cartilage Regeneration; Current Approaches and Future Perspectives

Cartilage regeneration with dual‐drug‐releasing injectable hydrogel/microparticle system: In vitro and in vivo study

Contact Info

Product

Resources

About