Silk Fibroin Films Decorated with Magnetic Nanoparticles for Wound Healling Applications

Bunea, Mihaela-Cristina; Vasile, Eugenia; Gălățeanu, Bianca; Hudiţă, Ariana; Serban, Mirela; Zaharia, Cătălin

doi:10.37358/mp.17.1.4791

Cited by 7 publications

(9 citation statements)

References 14 publications

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“…The effect of pH on the color development in ninhydrin method was previously studied [13] for a variety of amino acid concentrations and in a restricted pH range (5.3-8.05), while in the present study was specifically analyzed for amino acid lysine and in a large pH range (2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Apart from this, the ratio of Lys to Nin was also investigated and identified as the most important parameter affecting the reaction.…”

Section: Introductionmentioning

confidence: 89%

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A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry

Bouzid¹,

Filho²,

Marques³

et al. 2020

Rev. Chim.

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The methodology of the amino acid lysine (Lys) analysis using ninhydrin (Nin) reagent by spectrophotometric method was in-depth studied and improved. The optimization of time, Lys to Nin volume ratio and temperature were explicitly investigated. A specific wavelength of 479 nm was set according to the peak of the absorbance spectrum. The reaction time between Lys and Nin, in aqueous solution, occurs during 50 min. The Lys/Nin ratio 1.67 appeared most effective for this reaction. Moreover, the absorbance increased with temperature until a stable value was achieved nearly at 85�C. It was also found that the reaction is more efficient at initial pH 6 corresponding to formation of high amount of coloured product. Furthermore, the pH of post-reaction mixture was around 4 for different concentrations of lysine and initial pH range 3�10. For lysine solution contaminated with potassium (a representative interfering species) and after variation of the reaction conditions, the maximum wavelength (479 nm) was not affected. Mastering the use of these parameters leads to a good usage of this analytical technique which is simple, fast, accurate, less expensive and especially less harmful towards the environment and human health.

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Section: Introductionmentioning

confidence: 89%

“…Amino acids have also an essential role in regulating neural activities in vivo [7] and have a great importance in all basic biological processes in the cell [8]. They are also used in the synthesis of certain biomaterials such as silk fibroin-magnetite for wounds healing [9].…”

Section: Introductionmentioning

confidence: 99%

A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry

Bouzid¹,

Filho²,

Marques³

et al. 2020

Rev. Chim.

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“…The results showed low cytotoxicity to human progenitor cells with good antimicrobial activity, suggesting potential applications in tissue regeneration. Bunea et al developed a promising wound dressing based on a silk fibroin-IONP scaffold that exhibited good cytocompatibility on human ASCs [ 611 ]. Anghel et al modified a textile wound dressing by coating with a nanofluid containing IONPs and natural microbicidal compounds, such as vegetal eugenol and limonene [ 612 ].…”

Section: Other Soft Tissue Regeneration and Engineeringmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

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“…They are biocompatible, biodegradable, non-toxic, and thanks to the above properties, they are commonly used in biomedical applications (tissue repair, drug delivery, cellular therapy) [ 195 ]. There are very few reports of silk fibroin materials with the presence of magnetic particles: magnetic silk fibroin composite nanofibers, magnetic silk fibroin e-gel scaffolds, silk fibroin films decorated with magnetic particles, and silk fibroin/chitosan/magnetite scaffolds have been studied [ 196 , 197 , 198 , 199 ]. Silk fibroin-based materials with addition of noble metals (tetrachloropalladate (II) (Na 2 PdCl 4 ), potassium tetrachloroplatinate (II) (K 2 PtCl 4 ), gold chloride trihydrate (HAuCl 4 ·3H 2 O), silver nitrate (AgNO 3 ), tetrachloroauric acid (HAuCl 4 )) also can be found in the literature [ 200 , 201 ].…”

Section: Blendingmentioning

confidence: 99%

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Grabska-Zielińska

Sionkowska

2021

Materials

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This review supplies a report on fresh advances in the field of silk fibroin (SF) biopolymer and its blends with biopolymers as new biomaterials. The review also includes a subsection about silk fibroin mixtures with synthetic polymers. Silk fibroin is commonly used to receive biomaterials. However, the materials based on pure polymer present low mechanical parameters, and high enzymatic degradation rate. These properties can be problematic for tissue engineering applications. An increased interest in two- and three-component mixtures and chemically cross-linked materials has been observed due to their improved physico-chemical properties. These materials can be attractive and desirable for both academic, and, industrial attention because they expose improvements in properties required in the biomedical field. The structure, forms, methods of preparation, and some physico-chemical properties of silk fibroin are discussed in this review. Detailed examples are also given from scientific reports and practical experiments. The most common biopolymers: collagen (Coll), chitosan (CTS), alginate (AL), and hyaluronic acid (HA) are discussed as components of silk fibroin-based mixtures. Examples of binary and ternary mixtures, composites with the addition of magnetic particles, hydroxyapatite or titanium dioxide are also included and given. Additionally, the advantages and disadvantages of chemical, physical, and enzymatic cross-linking were demonstrated.

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Silk Fibroin Films Decorated with Magnetic Nanoparticles for Wound Healling Applications

Cited by 7 publications

References 14 publications

A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry

A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

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