Silk Fibroin Nanoparticles for Drug Delivery: Effect of Bovine Serum Albumin and Magnetic Nanoparticles Addition on Drug Encapsulation and Release

Gianak, Olga; Pavlidou, E.; Sarafidis, C.; Karageorgiou, Vassilis; Deliyanni, Eleni A.

doi:10.3390/separations5020025

Cited by 39 publications

(26 citation statements)

References 24 publications

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“…These interactions may be responsible for promoting the association of porphyrins with the fibroin fibers, as it is known that fibroin can form π–π stacking interactions with aromatic rings by means of tyrosine residues . It has been previously reporter in the literature that curcumin attached to fibroin nanoparticle via π–π stacking and this reduced curcumin release and in our case this mechanism could be possible being the responsible for the silk fibers/porphyrin interactions …”

Section: Resultssupporting

confidence: 52%

Silk fibroin hydrogels for potential applications in photodynamic therapy

et al. 2018

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In this study, we prepared translucid hydrogels with different concentrations of silk fibroin, extracted from raw silk fibers, and used them as a matrix to incorporate the photosensitizer 5-(4-aminophenyl)-10,15,20-tris-(4-sulphonatophenyl) porphyrin trisodium for application in photodynamic therapy (PDT). The hydrogels obtained were characterized by rheology, spectrophotometry, and scattering techniques to elucidate the factors involved in the formation of the hydrogel, and to characterize the behavior of silk fibroin (SF) after incorporating of the porphyrin to the matrix. The rheology results demonstrated that the SF hydrogels had a shear thinning behavior. In addition, we were able to verify that the structure of the material was able to be recovered over time after shear deformation. The encapsulation of porphyrins in hydrogels leads to the formation of self-assembled peptide nanostructures that prevent porphyrin aggregation, thereby greatly increasing the generation of singlet oxygen. Also, our findings suggest that porphyrin can diffuse out of the hydrogel and permeate the outer skin layers. This evidence suggests that SF hydrogels could be used as porphyrin encapsulation and as a drug carrier for the sustained release of photosensitizers for PDT. K E Y W O R D Sfibroin, hydrogel, photodynamic therapy, porphyrin, rheology, singlet oxygen

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Section: Resultssupporting

confidence: 52%

Silk fibroin hydrogels for potential applications in photodynamic therapy

et al. 2018

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“…Finding a suitable carrier that delivers the drugs and targets the tumor blood vessels' leaky vasculature passively is a practical way to make the chemotherapeutics effectively. 7 Serum albumin is a typical model protein, and because it can bind with various ligands, it is also called a multifunctional carrier protein of plasma. [8][9][10] Due to these characteristics, bovine serum albumin (BSA) is usually made into nanoparticles (NPs), which are biodegraded and tractable to be prepared in specific sizes.…”

Section: Introductionmentioning

confidence: 99%

<p>Novel Albumin Nanoparticle Enhanced the Anti-Insulin-Resistant-Hepatoma Activity of Metformin</p>

Liu

Lin

et al. 2020

IJN

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Introduction: Metformin is an ideal candidate to treat the liver tumor with insulin resistance because of its good performance in the treatment of type 2 diabetes and the advantage in cancer therapy. We aim to develop a delivery system with higher efficiency than free drug. Methods: Metformin-bovine serum albumin (met-BSA) nanoparticles (NPs) were prepared using the anti-solvent precipitation method with a stabilizer of BSA for particle growth. The therapeutic effect of the drug was tested by the insulin-resistant HepG2 cells and C57BL/6J mice at a glucose starvation condition. The interaction mechanism of the drug and the protein during the formation of the NPs was tested using a series of spectroscopy. Results: Metformin and BSA formed nonporous and spherical particles of about 200 nm with proper lognormal distribution and thermostability. The cellular uptake, as well as the anti-liver cancer activities of met-BSA, was enhanced dramatically compared with the free drug. The thermodynamic studies suggested that the weak binding of metformin to BSA was governed by hydrogen bonds and van der Waals forces. Moreover, the results of synchronous, circular dichroism (CD) and three-dimensional fluorescence demonstrated that the BSA skeleton and chromophore microenvironments were changed in the presence of metformin. Conclusion: Therefore, met-BSA has been proved as a simple yet effective therapeutic agent for cancer with insulin resistance, promising for future clinic translations in cancer treatment.

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“…XRD was employed to further characterize the magnetic silk fibroin nanoparticles. As shown in Figure b a broad peak around 2θ=20° was seen which could be attributed to the silk II crystal structure of fibroin . In addition, diffraction peaks corresponding to the (220), (311), (400), (440), (422), (511) and (440) planes are related to the crystal structure of Fe3O4 which confirm the formation of magnetic silk fibroin nanoparticles …”

Section: Resultsmentioning

confidence: 67%

Synthesis of Magnetic Silk Nanostructures with Peroxidase‐Like Activity as an Approach for the Detection of Glucose

et al. 2020

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Nanozymes have fascinated increasing attention in the field of enzyme based detection methods. In this study, we have focused on developing a rapid, colorimetric sensing method using peroxidase mimicking nanostructures for the visual detection of glucose molecules in biological samples. For this, using Fe3O4 and silk fibroin proteins, magnetic silk nanostructures (MSF nanostructures) were specifically tailored. Given their ability of catalyzing the oxidation of 3,3′,5,5′‐tetramethylbenzidine (TMB) in the presence of hydrogen peroxide, they can be used as colorimetric catalysts in the detection of glucose based on the production of H2O2. Using MSF nanostructures, glucose molecules were detected by both the naked eye and UV‐Vis spectrophotometry in concentrations ranging from 1.4 to 86.0 μM, with a detection limit of 0.5 μM and a good linear relationship (R2=0.988). In conclusion, it can be stated that the proposed nanostructure holds great potential for biosensing applications.

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Silk Fibroin Nanoparticles for Drug Delivery: Effect of Bovine Serum Albumin and Magnetic Nanoparticles Addition on Drug Encapsulation and Release

Cited by 39 publications

References 24 publications

Silk fibroin hydrogels for potential applications in photodynamic therapy

Silk fibroin hydrogels for potential applications in photodynamic therapy

<p>Novel Albumin Nanoparticle Enhanced the Anti-Insulin-Resistant-Hepatoma Activity of Metformin</p>

Synthesis of Magnetic Silk Nanostructures with Peroxidase‐Like Activity as an Approach for the Detection of Glucose

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