Synthesis of Magnetic Silk Nanostructures with Peroxidase‐Like Activity as an Approach for the Detection of Glucose

Abstract

Nanozymes have fascinated increasing attention in the field of enzyme based detection methods. In this study, we have focused on developing a rapid, colorimetric sensing method using peroxidase mimicking nanostructures for the visual detection of glucose molecules in biological samples. For this, using Fe3O4 and silk fibroin proteins, magnetic silk nanostructures (MSF nanostructures) were specifically tailored. Given their ability of catalyzing the oxidation of 3,3′,5,5′‐tetramethylbenzidine (TMB) in the presence of hydrogen peroxide, they can be used as colorimetric catalysts in the detection of glucose based on the production of H2O2. Using MSF nanostructures, glucose molecules were detected by both the naked eye and UV‐Vis spectrophotometry in concentrations ranging from 1.4 to 86.0 μM, with a detection limit of 0.5 μM and a good linear relationship (R2=0.988). In conclusion, it can be stated that the proposed nanostructure holds great potential for biosensing applications.

Peroxidase Effect of Ce2(WO4)3 Nanoparticles to Detection of Glucose as a Colorimetric Sensor

“…Human saliva was used to detect glucose concentrations in this assay [21] (Table 3). (All saliva samples were collected with the consent of volunteers from own laboratory).…”

“…[7][8][9][10][11][12][13] To date, an extensive range of nanozymes with enzymatic properties for detection of glucose has been reported; for instance, Au NPs, Ag NPs, Pt NPs and their alloys, [14] carbon-based nanomaterials, [15][16][17] metal-organic frameworks (MOFs), [18] Tungsten disulfide (WS 2 ) nanosheets, [15] etc. [3,[19][20][21] Recently, metal tungstate (MWO 4 ) has captivated extended research interest because of its unique interior structure, which leads to a high electronic conductivity with electron transitions within the 4 f shell. Furthermore, low cost property, has made tungstate a good candidate in diverse areas, such as scintillation detectors, optical fibers, laser host material, microwaves, catalysis, photoluminescence, and super capacitors.…”

“…Nanozymes have also been used in biosensors to detect different molecules, cancer cells, and other targets [7–13] . To date, an extensive range of nanozymes with enzymatic properties for detection of glucose has been reported; for instance, Au NPs, Ag NPs, Pt NPs and their alloys, [14] carbon‐based nanomaterials, [15–17] metal–organic frameworks (MOFs), [18] Tungsten disulfide (WS 2 ) nanosheets, [15] etc [3,19–21] . Recently, metal tungstate (MWO 4 ) has captivated extended research interest because of its unique interior structure, which leads to a high electronic conductivity with electron transitions within the 4 f shell.…”