Silk Fibroin/Tannin/ZnO Nanocomposite Hydrogel with Hemostatic Activities

Abstract

The inevitable bleeding and infections caused by disasters and accidents are the main causes of death owing to extrinsic trauma. Hemostatic agents are often used to quickly suppress bleeding and infection, and they can solve this problem in a short time. Silk fibroin (SF) has poor processibility in water, owing to incomplete solubility therein. In this study, aiming to overcome this disadvantage, a modified silk fibroin (SF-BGE), easily soluble in water, was prepared by introducing butyl glycidyl ether (BGE) into its side chain. Subsequently, a small amount of tannic acid (TA) was introduced to prepare an SF-BGE /TA solution, and ZnO nanoparticles (NPs) were added to the solution to form the coordination bonds between the ZnO and TA, leading to an SF-based nanocomposite hydrogel. A structural characterization of the SF-BGE, SF-BGE/TA, SF-BGE/TA/ZnO, and the coordination bonds between ZnO/TA was observed by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and the phase change was observed by rheological measurements. The pore formation of the SF-BGE/TA/ZnO hydrogel and dispersibility of ZnO were verified through energy-dispersive X-ray spectroscopy (EDS) and scanning electron microscopy (SEM). The cytocompatible and hemostatic performances of the SF-BGE/TA/ZnO NPs composite hydrogels were evaluated, and the hydrogels showed superior hemostatic and cytocompatible activities. Therefore, the SF-based nanocomposite hydrogel is considered as a promising material for hemostasis.

Silk Fibroin and Its Nanocomposites for Wound Care: A Comprehensive Review

“…Hydrogels take up large volumes of liquid to attain hemostasis in large arterial bleeds. Several research groups have developed SF-based adhesives by tannic acid (TA) modification − and photocuration. − The abundant phenolic moieties present in TA offer a strong binding affinity toward the mucosal layer. Bai et al .…”

“…Although fibrin-based sealants are available for clinical applications, they are ineffective under dynamic conditions (e.g., beating heart, shear of blood flow) due to their poor adhesive strength on bleeding tissues. 118 Further, in an attempt to develop multifunctional hemostatic hydrogel, Yang et al 119 combined modified SF with ZnO nanoparticles. Herein, the authors chemically modified SF by introducing butyl glycidyl ether (BGE) into its side chain to obtain a water-soluble derivative (SF-BGE) ( Figure 7 Aa).…”

Chitosan-Based Biomaterials for Hemostatic Applications: A Review of Recent Advances

“…Similar to copper nanoparticles, zinc nanoparticles have been found to promote blood clotting and prevent bleeding when applied to a wound. Several studies showed that zinc nanoparticles significantly accelerated blood clotting in vitro and demonstrated antibacterial activity [ 68 , 69 , 70 , 71 , 72 , 73 ].…”

Study of the Possibility of Using Sol–Gel Technology to Obtain Magnetic Nanoparticles Based on Transition Metal Ferrites

“…Research in the field of medicine is becoming more and more interdisciplinary, new methods of treatment are created. Interventional procedures are widely used [ 1 , 2 , 3 ], such as transarterial chemoembolization, catheter intra-arterial delivery of nanoparticles, etc. The possibilities of non-surgical and drug-free direct selective influence on individual cells in an external magnetic field by oscillations of magnetic nanoparticles fixed on the cell membranes or introduced into cells have been investigated [ 4 , 5 , 6 , 7 ].…”