Silk Sericin-Functionalized Bacterial Cellulose as a Potential Wound-Healing Biomaterial

Abstract

Bacterial cellulose (BC) is a polysaccharide known as a suitable matrix for proper wound healing. To improve this ability, BC was functionalized with silk sericin (SS) that has cytoprotective and mitogenic effects. The composites obtained by solution impregnation were stabilized by hydrogen bonds, and SS could be released in a controlled manner. The constructs were highly porous with interconnected pores allowing for high water uptake that varied with the SS concentration used for sample preparation. While SS did not disrupt the stability of the BC network, soluble SS diffusing from the composites did not influence keratinocyte growth but enhanced fibroblast proliferation, which would further optimize the wound healing process and improve extracellular matrix production, accelerating healing. Further, improved cell viability was observed upon the composites. Because of their attractive structure and properties, these BC-SS biomaterials represent potential candidates not only for wound dressing applications but also for tissue engineering.

Composites of Bacterial Cellulose and Small Molecule-Decorated Gold Nanoparticles for Treating Gram-Negative Bacteria-Infected Wounds

“…The water uptake ability was studied by gravimetric method . The water uptake by the membranes was determined by the equation: w = ( w s − w d )/ w d , where w d is the weight of the dry membrane and w s is the wet weight at different times …”

Fabrication of Bacterial Cellulose-Curcumin Nanocomposite as a Novel Dressing for Partial Thickness Skin Burn

“…To this end, the use of bacterial cellulose (BC), produced by microbial cells ( Ullah et al, 2017 ) and cell-free systems ( Ullah et al, 2015 ; Kim et al, 2019 ), has received a growing interest as a biomaterial in various biomedical applications ( Klemm et al, 2001 ; Czaja et al, 2006 ; Khan et al, 2015a , b ; Hussain et al, 2019 ), specialty membrane ( Rajwade et al, 2015 ), biosensors ( Jasim et al, 2017 ; Farooq et al, 2020 ), and drug release ( Numata et al, 2015 ; Li et al, 2018 ). These applications utilize both pure BC and its composites with other materials, such as biopolymers including collagen ( Takeda et al, 2016 ), silk-sericin ( Lamboni et al, 2016 ), gelatin ( Khan et al, 2018 ), alginate ( Kirdponpattara et al, 2015 ), and chitosan ( Ul-Islam et al, 2019 ), and nanoparticles such as silver ( Maneerung et al, 2008 ), zinc ( Ul-Islam et al, 2014 ; Khalid et al, 2017a ), titanium dioxide ( Khan et al, 2015a , b ; Ullah et al, 2016a ; Khalid et al, 2017b ), and gold ( Khan et al, 2018 ), as well as clay materials such as pristine and modified montmorillonite ( Ul-Islam et al, 2013b ). The unique features of BC include high purity, better mechanical properties, high water holding capacity (WHC) and slow water release rate (WRR), active surface area, moderate biocompatibility, biodegradability, three-dimensional (3D) reticulate fibrous structure, micro-porosity, optical transparency, and non-toxicity, and moldability into different shapes ( Czaja et al, 2006 ; Ul-Islam et al, 2013a ; Ullah et al, 2016b ; Di et al, 2017 ).…”

Effect of Quercetin Incorporated Silk Sericin/Gelatin Scaffolds in Wound Healing

“…Silk sericin solutions display maximum absorbance at 276 nm, which is similar to previous reports. 30 Gelatin shows maximum absorbance at 272 nm and quercetin at 372.97 nm, SS/G at 278 nm, which indicates that silk sericin and gelatin have merged together at one peak, since both sample are proteins and SS/G/Q at 278 nm and 372.92 nm that indicates the successful incorporation of quercetin.…”