Silver-Enhanced Colloidal Gold for Light Microscopy

Preparation, use, and enlargement of ultrasmall gold particles in immunoelectron microscopy

“…Also, pretreatment with Lugol's iodine (with controversial effects and unknown reaction mechanisms; see Scopsi, 1989;Hacker, 1989) has been reported to induce silver staining of collagen when using a silver lactatecontaining enhancer . Hacker (1989) reported that parabenzoquinone, used as a fixative, interferes with the silver enhancement process.…”

“…Here, the metallic silver, which is produced by chemical reduction of silver ions, deposits on the surface of the gold colloids, which serve as nuclei for microcrystallization (for reviews, see Hacker, 1989;Larsson, 1989;Scopsi, 1989;Danscher et al, 1995;Hacker et al, 1995). In contrast to photographic development, where the composition of the film emulsion and the development process are well defined, the enlargement of gold labels on biological specimens is performed rather empirically.…”

Immunocytochemical investigation of insulin secretion by pancreatic beta-cells in control and diabetic Psammomys obesus.

“…To reveal the immunostaining. the protein A-gold method followed by silver enhancement was performed (49). The sections were incubated with a protein A-gold complex prepared with 15-nm gold particles, as previously described (50).…”

Polymerase Chain Reaction (PCR) In Situ Hybridization: Detection of Human Papillomavirus (HPV) DNA in SiHa Cell Monolayers

“…For this, the specimens are immersed in a freshly prepared solution consisting of 100 mg silver acetate (code 85140, Fluka, FRO) in 50 ml deionized, glass distilled water, and 250 mg hydroquinone in 50 ml citrate buffer (23.5 g trisodium citrate dihydrate and 25.5 g citric acid monohydrate in 850 ml deionized, glass distilled water, pH 3.8). 34,35 Counterstaining may be performed with Mayer's hematoxylin andlor eosin andlor nuclear fast red. Dehydrate in graded alcohols, clear in xylene and mount in DPX (BDH-Biochemicals, UK) or Canada balm or cytoseal (Curtin Matheson Scientific In., Wayne, NJ, USA).…”