Silver Impregnation Stain for
            <i>Leptospira</i>
            and Flagella

Blenden, Donald C.; Goldberg, Herbert S.

doi:10.1128/jb.89.3.899-900.1965

Cited by 95 publications

(29 citation statements)

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“…2 and 3). The organism is not motile under the conditions described here, and no evidence of flagella was found although several stains were used (4,9,23). Spore stains were made on cultures at different stages of growth, as well as after heat and oxygen treatments, and all results were negative.…”

Section: Resultsmentioning

confidence: 84%

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen1

Huisingh

McNeill

Matrone

1974

Applied Microbiology

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Several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. One isolate, strain D, was selected for characterization. This organism is a nonsporeforming, obligately anaerobic, mesophilic, nonmotile, gram-negative, straight rod. Cell-free extracts show absorption maxima for cytochrome c3 and desulfoviridin, characteristic of Desulfovibrio. Carbohydrates, as a sole carbon source, will support growth. Lactate supports growth in the presence of sulfate, not in its absence, whereas glucose or pyruvate support growth either in the presence or absence of sulfate. The isolate has a deoxyribonucleic acid base composition of 61.2% guanine plus cytosine, which is similar to that of several other species of Desulfovibrio; however, it differs from previously described species in morphology, motility, and carbon source utilization. Cell-free extracts of this bacterium exhibit adenosine 5'-triphosphate-sulfurylase, adenosine-5'-phosphosulfate-reductase, and hydrogenase activity. After incubation of cell-free extracts with adenine 5'-triphosphate and 3SO042-, adenosine-5'-phosphosulfate rather than 3'-phosphoadenosine-5'-phosphosulfate was shown to be labeled, indicating that the pathway of sulfate reduction in this organism is similar to that of other dissimilatory sulfate reducers. This is the first report of a Desulfovibrio sp. isolated from the rumen.

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Section: Resultsmentioning

confidence: 84%

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen1

Huisingh

McNeill

Matrone

1974

Applied Microbiology

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“…Staining to visualize general flagellation by the silver impregnation method was carried out essentially as described earlier (Blenden and Goldberg, 1965). For colocalization of mCherry-tagged stator subunits with the flagellum, the flagellar filaments were visualized by fluorescence labelling using Alexa Fluor 488 carboxylic acid succinimidyl ester (Invitrogen) essentially as previously described (Turner et al, 2000).…”

Section: Flagella Stainingmentioning

confidence: 99%

Two different stator systems drive a single polar flagellum in Shewanella oneidensis MR‐1

Paulick

Koerdt

Lassak

et al. 2009

Molecular Microbiology

130

137

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SummaryThe Gram-negative metal ion-reducing bacterium Shewanella oneidensis MR-1 is motile by means of a single polar flagellum. We identified two potential stator systems, PomAB and MotAB, each individually sufficient as a force generator to drive flagellar rotation. Physiological studies indicate that PomAB is sodium-dependent while MotAB is powered by the proton motive force. Flagellar function mainly depends on the PomAB stator; however, the presence of both stator systems under low-sodium conditions results in a faster swimming phenotype. Based on stator homology analysis we speculate that MotAB has been acquired by lateral gene transfer as a consequence of adaptation to a low-sodium environment. Expression analysis at the single cell level showed that both stator systems are expressed simultaneously. An active PomB-mCherry fusion protein effectively localized to the flagellated cell pole in 70-80% of the population independent of sodium concentrations. In contrast, polar localization of MotB-mCherry increased with decreasing sodium concentrations. In the absence of the Pom stator, MotB-mCherry localized to the flagellated cell pole independently of the sodium concentration but was rapidly displaced upon expression of PomAB. We propose that selection of the stator occurs at the level of protein localization in response to sodium concentrations.

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“…The shape and motility of cultures from the lag, exponential and stationary phases of growth were observed by phase-contrast microscopy (1000i). Motility was determined by the hanging-drop method and staining of the flagellum was by the silver-impregnation method (Blenden & Goldberg, 1965). All biochemical tests were performed by growing the culture at 20 mC.…”

Section: Morphology Motility and Biochemical Characteristicsmentioning

confidence: 99%

Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.

Reddy¹,

Prakash²,

Matsumoto³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Strain CMS 90rT, a red-pigmented bacterium, was isolated from a cyanobacterial mat sample from a pond located in McMurdo, Antarctica. Based on its chemotaxonomic and phylogenetic properties, strain CMS 90r(T) was identified as a member of group I of Arthrobacter. It shared 16S rDNA similarity of 98% with Arthrobacter oxydans ATCC 14358T and Arthrobacter polychromogenes ATCC 15216T, while DNA-DNA similarities determined for these three organisms were less than 70%. It also differed from all 17 reported Arthrobacter species with A3alpha-variant peptidoglycan in that it possessed a unique peptidoglycan (Lys-Gly-Ala3) and contained galactose, glucose, ribose and rhamnose as cell-wall sugars. These data and the presence of diagnostic phenotypic traits support the description of CMS 90r(T) as a novel species of Arthrobacter, for which the name Arthrobacter roseus sp. nov. is proposed. The type strain is strain CMS 90r(T) (= MTCC 3712T = DSM 14508T).

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Silver Impregnation Stain for Leptospira and Flagella

Abstract: Leptospires are difficult to stain with the usual bacterial stains; many different stains have been proposed, most of which are designed for staining of tissue sections. Silver stains that are available for smear preparations yield variable, unreliable

Cited by 95 publications

References 0 publications

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen1

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen1

Two different stator systems drive a single polar flagellum in Shewanella oneidensis MR‐1

Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.

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