Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine

Hu, Jiang; Li, Xiangmin; Xiong, Ying; Pei, Ke; Nie, Lijuan; Xiong, Yonghua

doi:10.3390/toxins9030083

Cited by 58 publications

(22 citation statements)

References 29 publications

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“…This weaker attachment of the aptamer to the surface favors the aptamer–OTA interaction, the displacement of the aptamer, and cargo delivery. However, both LOD values comply with those established by the legislation and are similar to or better than most published techniques for the detection of OTA (see Table S4) …”

Section: Resultssupporting

confidence: 83%

“…This weaker attachment of the aptamer to the surfacef avors the aptamer-OTAi nteraction, the displacement of the aptamer,a nd cargo delivery.H owever,b oth LOD values comply with those established by the legislation and are similar to or better than most published techniques for the detection of OTA( see Ta ble S4). [42][43][44][45][46][47][48][49][50][51][52][53][54] Additionally,t he selectivity of S3 (Figure 3i nb lack) and S5 ( Figure 3i ng rey) to OTAw as also studied. With this aim, cargo releasefrom S3 and S5 was testedi np resence of OTA, fumonisin B1 and aflatoxin B1.…”

Section: Sensitivity and Selectivity Studiesmentioning

confidence: 99%

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Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

et al. 2017

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Aptamers have been used as recognition elements for several molecules due to their great affinity and selectivity. Additionally, mesoporous nanomaterials have demonstrated great potential in sensing applications. Based on these concepts, we report herein the use of two aptamer‐capped mesoporous silica materials for the selective detection of ochratoxin A (OTA). A specific aptamer for OTA was used to block the pores of rhodamine B‐loaded mesoporous silica nanoparticles. Two solids were prepared in which the aptamer capped the porous scaffolds by using a covalent or electrostatic approach. Whereas the prepared materials remained capped in water, dye delivery was selectively observed in the presence of OTA. The protocol showed excellent analytical performance in terms of sensitivity (limit of detection: 0.5–0.05 nm), reproducibility, and selectivity. Moreover, the aptasensors were tested for OTA detection in commercial foodstuff matrices, which demonstrated their potential applicability in real samples.

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Section: Resultssupporting

confidence: 83%

Section: Sensitivity and Selectivity Studiesmentioning

confidence: 99%

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

et al. 2017

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“…Such pairs can be two kinds of nanoparticles attached to different immunoreagents. Thus, Shi et al [83] successfully used for this purpose quantum dots and gold nanoparticles in the analysis of ractopamine, Anfossi et al [84]-quantum dots and gold or silver nanoparticles in the analysis of fumonisin, and Jiang et al [85]-ruthenium-doped silicon nanoparticles and silver nanoparticles in the analysis of ochratoxin A. Another perspective approach is open sandwich immunoassay (OSI).…”

Section: Proper Interaction For Lfiamentioning

confidence: 99%

“…Systems using fluorescein isothiocyanate and gold nanoparticles were developed by Wang et al for the detection of cancer embryonic antigens [108]. Other variants of fluorescent LFIA were also described, for example, registration of background fluorescence quenching in Chen et al [109], silver nanoparticle-based fluorescence quenching in Jiang et al [85], and quenching of the fluorescence of quantum dots by gold and silver nanoparticles in Anfossi et al [84]. (See also Section 5 with their consideration as examples of competitive immunoassays with a direct dependence of the detected signal on the analyte content.…”

Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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“…Taking ochratoxin A (OTA) as an example, Zhu et al developed an ELISA method by using the conjugation of botryoid-shaped Au/Ag nanoparticles and second antibodies connected with horseradish peroxidase (HRP) [2]. Hu et al developed a silver nanoparticle-based fluorescence-quenching lateral flow immunoassay based on the monoclonal antibody [3]. Tang et al developed a noncompetitive and homogeneous fluorescence resonance energy transfer immunoassay using nanobodies [4].…”

Section: Introductionmentioning

confidence: 99%

Change of Amino Acid Residues in Idiotypic Nanobodies Enhanced the Sensitivity of Competitive Enzyme Immunoassay for Mycotoxin Ochratoxin A in Cereals

Zhang

Tang

et al. 2020

Toxins

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Anti-idiotypic nanobodies, usually expressed by gene engineering protocol, has been shown as a nontoxic coating antigen for toxic compound immunoassays. We here focused on how to increase immunoassay sensitivity by changing the nanobody's primary sequence. In the experiments, two anti-idiotype nanobodies against monoclonal antibody 1H2, which is specific to ochratoxin A, were obtained and named as nontoxic coating antigen 1 (NCA1) and nontoxic coating antigen 2 (NCA2). Three differences between the nanobodies were discovered. First, there are six amino acid residues (AAR) of changes in the complementarity determining region (CDR), which compose the antigen-binding site. One of them locates in CDR1 (I-L), two of them in CDR2 (G-D, E-K), and three of them in CDR3 (Y-H, Y-W). Second, the affinity constant of NCA1 was tested as 1.20 × 10 8 L mol −1 , which is about 4 times lower than that of NCA2 (5.36 × 10 8 L mol −1 ). Third, the sensitivity (50% inhibition concentration) of NCA1 for OTA was shown as 0.052 ng mL −1 , which was 3.5 times lower than that of nontoxic coating antigen 2 (0.015 ng mL −1 ). The results indicate that the AAR changes in CDR of the anti-idiotypic nanobodies, from nonpolar to polar, increasing the affinity constant may enhance the immunoassay sensitivity. In addition, by using the nontoxic coating antigen 2 to substitute the routine synthetic toxic antigen, we established an eco-friendly and green enzyme-linked immunosorbent assay (ELISA) method for rapid detection of ochratoxin A in cereals. The half-maximal inhibitory concentration (IC 50 ) of optimized ELISA was 0.017 ng mL −1 with a limit of detection (LOD) of 0.003 ng mL −1 . The optimized immunoassay showed that the average recoveries of spiked corn, rice, and wheat were between 80% and 114.8%, with the relative standard deviation (RSD) ranging from 3.1-12.3%. Therefore, we provided not only basic knowledge on how to improve the structure of anti-idiotype nanobody for increasing assay sensitivity, but also an available eco-friendly ELISA for ochratoxin A in cereals.Key Contribution: Two anti-idiotype nanobodies were developed and used as non-toxic antigens against monoclonal antibody 1H2, which is specific to ochratoxin A. A basic knowledge was discovered Toxins 2020, 12, 273 2 of 15 that the change of amino acid residues in idiotypic nanobodies enhanced the sensitivity by comparing the amino acid sequences, structure, affinity constants, and sensitivities between both nanobodies.

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Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine

Cited by 58 publications

References 29 publications

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Change of Amino Acid Residues in Idiotypic Nanobodies Enhanced the Sensitivity of Competitive Enzyme Immunoassay for Mycotoxin Ochratoxin A in Cereals

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