Silver Nanoparticle-Enhanced Fluorescence Resonance Energy Transfer Sensor for Human Platelet-Derived Growth Factor-BB Detection

Abstract

A silver nanoparticle (AgNP)-enhanced fluorescence resonance energy transfer (FRET) sensing system is designed for the sensitive detection of human platelet-derived growth factor-BB (PDGF-BB). Fluorophore-functionalized aptamers and quencher-carrying strands hybridized in duplex are coupled with streptavidin (SA)-functionalized nanoparticles to form a AgNP-enhanced FRET sensor. The resulting sensor shows lower background fluorescence intensity in the duplex state due to the FRET effect between fluorophores and quenchers. Upon the addition of PDGF-BB, the quencher-carrying strands (BHQ-2) of the duplex are displaced leading to the disruption of the FRET effect. As a result, the fluorescent intensity of the fluorophore-aptamer within the proximity of the AgNP is increased. When compared to the gold nanoparticle (AuNP)-based FRET and bare FRET sensors, the AgNP-based FRET sensor showed remarkable increase in fluorescence intensity, target specificity, and sensitivity. Results also show versatility of the AgNP in the enhancement of sensitivity and selectivity of the FRET sensor. In addition, a good linear response was obtained when the PDGF-BB concentrations are in the ranges of 100-500 and 6.2-50 ng/mL with the detection limit of 0.8 ng/mL.

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Silver nanoparticles-enhanced rare earth co-luminescence effect of Tb(III)–Y(III)–dopamine system

“…Silver nanoparticles (AgNPs) have unique optoelectronic properties, such as localized surface plasmon resonance (LSPR), surface-enhanced Raman and surface-enhanced fluorescence [9][10][11].…”

“…These optoelectronic properties have been widely used in bioanalytical researches [11][12][13]. Metal enhanced fluorescence (MEF) [11][12][13][14] occurs due to the couple interaction between fluorophores and the plasmon resonance of metal nanoparticles, resulting in an increase in both the radiative decay rate and fluorescence emission.…”

“…Metal enhanced fluorescence (MEF) [11][12][13][14] occurs due to the couple interaction between fluorophores and the plasmon resonance of metal nanoparticles, resulting in an increase in both the radiative decay rate and fluorescence emission. Studies showed that MEF property can be affected by nanoparticle size, particle shape, spacing distance of fluorophore-tometal, and wavelength [11,14,15]. Dopamine (DA), 4-(2-aminoethyl)-benzen-1, 2-diol, a natural substance, is one of the crucial catecholamines.…”

Aptamer and rolling circle amplification-involved sandwich assay for platelet-derived growth factor-BB with absorbance analysis

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