Silver nanoparticle induced toxicity to human sperm by increasing ROS(reactive oxygen species) production and DNA damage

Wang, Enyin; Huang, Yan; Du, Qianming; Sun, Yingpu

doi:10.1016/j.etap.2017.04.010

Cited by 73 publications

(46 citation statements)

References 30 publications

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“…vinelandii growth after AgNPs exposure. Recent studies suggest that ROS generation and oxidative stress are the likely mechanism of AgNPs toxicity [58]. There are antioxidant systems in the cells, which mainly include antioxidant enzymes and antioxidants such as SOD and GSH [59].…”

Section: Resultsmentioning

confidence: 99%

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Zhang

et al. 2018

PLoS ONE

141

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The influence of nanomaterials on the ecological environment is becoming an increasingly hot research field, and many researchers are exploring the mechanisms of nanomaterial toxicity on microorganisms. Herein, we studied the effect of two different sizes of nanosilver (10 nm and 50 nm) on the soil nitrogen fixation by the model bacteria Azotobacter vinelandii. Smaller size AgNPs correlated with higher toxicity, which was evident from reduced cell numbers. Flow cytometry analysis further confirmed this finding, which was carried out with the same concentration of 10 mg/L for 12 h, the apoptotic rates were20.23% and 3.14% for 10 nm and 50 nm AgNPs, respectively. Structural damage to cells were obvious under scanning electron microscopy. Nitrogenase activity and gene expression assays revealed that AgNPs could inhibit the nitrogen fixation of A. vinelandii. The presence of AgNPs caused intracellular reactive oxygen species (ROS) production and electron spin resonance further demonstrated that AgNPs generated hydroxyl radicals, and that AgNPs could cause oxidative damage to bacteria. A combination of Ag content distribution assays and transmission electron microscopy indicated that AgNPs were internalized in A. vinelandii cells. Overall, this study suggested that the toxicity of AgNPs was size and concentration dependent, and the mechanism of antibacterial effects was determined to involve damage to cell membranes and production of reactive oxygen species leading to enzyme inactivation, gene down-regulation and death by apoptosis.

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Section: Resultsmentioning

confidence: 99%

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Zhang

et al. 2018

PLoS ONE

141

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“…Reactive oxygen species (ROS) are highly reactive and unstable. The accumulation of ROS may lead to the oxidation of cellular components when the level is beyond the elimination ability of antioxidants [ 232 ], giving rise to oxidative stress. Oxidative stress may cause many chronic diseases, such as cancer, diabetics, cardiovascular diseases, and other degenerative diseases [ 233 ].…”

Section: Health Effects Associated With Pm Invasionmentioning

confidence: 99%

Interactions of particulate matter and pulmonary surfactant: Implications for human health

Wang

Liu

Zeng

2020

Advances in Colloid and Interface Science

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Particulate matter (PM), which is the primary contributor to air pollution, has become a pervasive global health threat. When PM enters into a respiratory tract, the first body tissues to be directly exposed are the cells of respiratory tissues and pulmonary surfactant. Pulmonary surfactant is a pivotal component to modulate surface tension of alveoli during respiration. Many studies have proved that PM would interact with pulmonary surfactant to affect the alveolar activity, and meanwhile, pulmonary surfactant would be adsorbed to the surface of PM to change the toxic effect of PM. This review focuses on recent studies of the interactions between micro/nanoparticles (synthesized and environmental particles) and pulmonary surfactant (natural surfactant and its models), as well as the health effects caused by PM through a few significant aspects, such as surface properties of PM, including size, surface charge, hydrophobicity, shape, chemical nature, etc. Moreover, in vitro and in vivo studies have shown that PM leads to oxidative stress, inflammatory response, fibrosis, and cancerization in living bodies. By providing a comprehensive picture of PM-surfactant interaction, this review will benefit both researchers for further studies and policy-makers for setting up more appropriate regulations to reduce the adverse effects of PM on public health.

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“…Furthermore, SiO 2 and CuO NPs were found to have different abilities to generate ROS and to induce cytotoxicity in pulmonary epithelial cells . In addition, Wang et al observed ROS production and DNA damage increase after Silver NPs exposure in human sperm.…”

Section: Introductionmentioning

confidence: 99%

The comparative study of the effects of Fe₂O₃ and TiO₂ micro‐ and nanoparticles on oxidative states of lung and bone marrow tissues and colony stimulating factor secretion

Soltani

Kahkhaie

Haftcheshmeh

et al. 2018

J of Cellular Biochemistry

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Nowadays, increased use of nanomaterials in industry and biomedicine poses potential risks to human health and the environment. Studying their possible toxicological effects is therefore of great significance. The present investigation was designed to examine the status of oxidative stress induced by nanoparticles (NPs) of ferric oxide (Fe2O 3) and titanium oxide (TiO 2) with their micro‐sized counterpart on mouse lung and bone marrow–derived normal tissue cells. We assessed the induction of oxidative stress by measuring its indicators such as antioxidant scavenging activity of superoxide dismutase and catalase as well as malondialdehyde concentration. Moreover, colony formation of bone marrow cells was assayed following induction with colony stimulating factor (CSF) from lung cells. NPs had a more potent stimulatory effect on the oxidative stress status than their micron‐sized counterparts. In addition, the highest level of oxidative stress derived from TiO 2 NPs was observed in both tissue types. Cotreatment with NPs and the antioxidant α‐tocopherol reduced antioxidant activities and membrane lipid peroxidation (LPO) in the lung cells, but increased CSF‐induced colony formation activity of bone marrow cells, suggesting that oxidative stress may be the cause of the cytotoxic effects of NPs. It is concluded that free radicals generated following exposure to NPs resulted in signiﬁcant oxidative stress in mouse cells, indicated by increased LPO and antioxidant enzyme activity and decreased colony formation.

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Silver nanoparticle induced toxicity to human sperm by increasing ROS(reactive oxygen species) production and DNA damage

Cited by 73 publications

References 30 publications

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Interactions of particulate matter and pulmonary surfactant: Implications for human health

The comparative study of the effects of Fe₂O₃ and TiO₂ micro‐ and nanoparticles on oxidative states of lung and bone marrow tissues and colony stimulating factor secretion

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Silver nanoparticle induced toxicity to human sperm by increasing ROS(reactive oxygen species) production and DNA damage

Cited by 73 publications

References 30 publications

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Interactions of particulate matter and pulmonary surfactant: Implications for human health

The comparative study of the effects of Fe2O3 and TiO2 micro‐ and nanoparticles on oxidative states of lung and bone marrow tissues and colony stimulating factor secretion

Contact Info

Product

Resources

About

The comparative study of the effects of Fe₂O₃ and TiO₂ micro‐ and nanoparticles on oxidative states of lung and bone marrow tissues and colony stimulating factor secretion