Silver Nanoparticles as Potent Multidrug-Resistant Incorporants in Biomedicine

Uddin, Imran; Parimi, Divya S.; Bollu, Tarun K.; Bhatt, Chandra S.; Suresh, Anil K.

doi:10.1007/978-3-030-84126-3_21

Cited by 6 publications

(5 citation statements)

References 51 publications

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“…Figure 3j depicts the utilization of E. coli and S. aureus , both gram-negative and gram-positive bacteria, as representative microorganisms to investigate the in vitro antibacterial efficacy of diverse nanofibrous membranes. Similarly, many studies have reported the effectiveness of AgNPs against multidrug-resistant bacterial strains, such as MRSA [ 50 ]. Both the PLLA/Ag and PLLA/C/Ag membranes exhibited similar results and showed a certain effect against MRSA ( Figure S6 , see online supplementary material).…”

Section: Resultsmentioning

confidence: 99%

“…First, AgNPs can penetrate the outer membrane of bacteria, increasing membrane permeability and leading to the leakage of cellular contents and ultimately cell death [ 69 , 70 ]. Second, AgNPs have an affinity for the sulfur or phosphorus groups present in intracellular components such as DNA and proteins, and thus the AgNPs can alter the structure and function of these components [ 50 , 71–73 ]. Third, the silver ions released from AgNPs can interact with cellular components such as membranes and nucleic acids [ 50 , 71–73 ].…”

Section: Discussionmentioning

confidence: 99%

“…Second, AgNPs have an affinity for the sulfur or phosphorus groups present in intracellular components such as DNA and proteins, and thus the AgNPs can alter the structure and function of these components [ 50 , 71–73 ]. Third, the silver ions released from AgNPs can interact with cellular components such as membranes and nucleic acids [ 50 , 71–73 ]. Moreover, AgNPs embedded in wound dressings, such as hydrogels and nanofibrous membranes, can also exhibit bacteriostatic effects [ 72 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

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Immunomodulatory poly(L-lactic acid) nanofibrous membranes promote diabetic wound healing by inhibiting inflammation, oxidation and bacterial infection

Wu,

Zhang,

Lin

et al. 2024

Burns &Amp; Trauma

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Background Given the significant impact on human health, it is imperative to develop novel treatment approaches for diabetic wounds, which are prevalent and serious complications of diabetes. The diabetic wound microenvironment has a high level of reactive oxygen species (ROS) and an imbalance between proinflammatory and anti-inflammatory cells/factors, which hamper the healing of chronic wounds. This study aimed to develop poly(L-lactic acid) (PLLA) nanofibrous membranes incorporating curcumin and silver nanoparticles (AgNPs), defined as PLLA/C/Ag, for diabetic wound healing. Methods PLLA/C/Ag were fabricated via an air-jet spinning approach. The membranes underwent preparation and characterization through various techniques including Fourier-transform infrared spectroscopy, measurement of water contact angle, X-ray photoelectron spectroscopy, X-ray diffraction, scanning electron microscopy, assessment of in vitro release of curcumin and Ag+, testing of mechanical strength, flexibility, water absorption and biodegradability. In addition, the antioxidant, antibacterial and anti-inflammatory properties of the membranes were evaluated in vitro, and the ability of the membranes to heal wounds was tested in vivo using diabetic mice. Results Loose hydrophilic nanofibrous membranes with uniform fibre sizes were prepared through air-jet spinning. The membranes enabled the efficient and sustained release of curcumin. More importantly, antibacterial AgNPs were successfully reduced in situ from AgNO3. The incorporation of AgNPs endowed the membrane with superior antibacterial activity, and the bioactivities of curcumin and the AgNPs gave the membrane efficient ROS scavenging and immunomodulatory effects, which protected cells from oxidative damage and reduced inflammation. Further results from animal studies indicated that the PLLA/C/Ag membranes had the most efficient wound healing properties, which were achieved by stimulating angiogenesis and collagen deposition and inhibiting inflammation. Conclusions In this research, we successfully fabricated PLLA/C/Ag membranes that possess properties of antioxidants, antibacterial agents and anti-inflammatory agents, which can aid in the process of wound healing. Modulating wound inflammation, these new PLLA/C/Ag membranes serve as a novel dressing to enhance the healing of diabetic wounds.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Immunomodulatory poly(L-lactic acid) nanofibrous membranes promote diabetic wound healing by inhibiting inflammation, oxidation and bacterial infection

Wu,

Zhang,

Lin

et al. 2024

Burns &Amp; Trauma

View full text Add to dashboard Cite

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“…AgNPs’ nanoscale size also allows them to pass through bacterial cell walls and alter the structure of the cell membrane. Organelle rupture and potential cell lysis can result from the cytoplasmic membrane becoming denatured ( Uddin et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Biogenic silver nanoparticles eradicate of Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) isolated from the sputum of COVID-19 patients

et al. 2023

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In recent investigations, secondary bacterial infections were found to be strongly related to mortality in COVID-19 patients. In addition, Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) bacteria played an important role in the series of bacterial infections that accompany infection in COVID-19. The objective of the present study was to investigate the ability of biosynthesized silver nanoparticles from strawberries (Fragaria ananassa L.) leaf extract without a chemical catalyst to inhibit Gram-negative P. aeruginosa and Gram-positive Staph aureus isolated from COVID-19 patient’s sputum. A wide range of measurements was performed on the synthesized AgNPs, including UV–vis, SEM, TEM, EDX, DLS, ζ -potential, XRD, and FTIR. UV-Visible spectral showed the absorbance at the wavelength 398 nm with an increase in the color intensity of the mixture after 8 h passed at the time of preparation confirming the high stability of the FA-AgNPs in the dark at room temperature. SEM and TEM measurements confirmed AgNPs with size ranges of ∼40-∼50 nm, whereas the DLS study confirmed their average hydrodynamic size as ∼53 nm. Furthermore, Ag NPs. EDX analysis showed the presence of the following elements: oxygen (40.46%), and silver (59.54%). Biosynthesized FA-AgNPs (ζ = −17.5 ± 3.1 mV) showed concentration-dependent antimicrobial activity for 48 h in both pathogenic strains. MTT tests showed concentration-dependent and line-specific effects of FA-AgNPs on cancer MCF-7 and normal liver WRL-68 cell cultures. According to the results, synthetic FA-AgNPs obtained through an environmentally friendly biological process are inexpensive and may inhibit the growth of bacteria isolated from COVID-19 patients.

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“…The mode of AgNPs antibacterial action remains to be elucidated, but there are several mechanisms proposed. Some scientists suggest that these particles adhere to the bacterial cell membrane and degrade the lipopolysaccharide molecules in the outer membrane of gram-negative bacteria which results in the formation of pores in the membrane, altering the membrane permeability and respiratory functions of the cells [37,38]. There is another plausible mechanism of their action: AgNPs could release toxic Ag + ions which react with thiol groups of proteins present in the cell, leading to the activation of reactive oxygen species (ROS) that cause oxidative damage to the microbial cell.…”

Section: Discussionmentioning

confidence: 99%

Bacteria-mediated synthesis of silver nanoparticles under photo-irradiation and their efficacy against methicillin-resistant Staphylococcus aureus

Malik,

Saleem,

Usama

et al. 2023

Microbiology Independent Research Journal (MIR Journal)

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Over the last few decades, morbidity and mortality rates from infectious diseases have increased due to antibiotic resistance. Community-acquired and nosocomial-related methicillin-resistant bacterial strains are proliferating, which makes the discovery of new antibiotics and the search for new approaches to fight pathogenic bacteria an urgent task. One of the new promising classes of antimicrobials is silver nanoparticles (AgNPs). The goal of this study was to investigate the bacteria-mediated synthesis of AgNPs and evaluate their antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). Indigenous bacteria from soil samples from Multan (Pakistan) were isolated and three strains – Klebsiella spp., Citrobacter freundii, and Bacillus cereus – were selected for the synthesis of AgNPs under photo-irradiation. The biosynthesis of AgNPs was confirmed by UV-visible spectroscopy. The AgNPs obtained in our experiments showed strong antibacterial activity against MRSA isolated from patients with community-acquired skin infections (from the dermatology ward of Jinnah Hospital Lahore) with 15 mm to 25 mm zones of inhibition (ZOI) according to the well diffusion method. The results of this project showed that microbial reduction of AgNO3 under photo-irradiation produces AgNPs that are highly active against MRSA.

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Silver Nanoparticles as Potent Multidrug-Resistant Incorporants in Biomedicine

Cited by 6 publications

References 51 publications

Immunomodulatory poly(L-lactic acid) nanofibrous membranes promote diabetic wound healing by inhibiting inflammation, oxidation and bacterial infection

Immunomodulatory poly(L-lactic acid) nanofibrous membranes promote diabetic wound healing by inhibiting inflammation, oxidation and bacterial infection

Biogenic silver nanoparticles eradicate of Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) isolated from the sputum of COVID-19 patients

Bacteria-mediated synthesis of silver nanoparticles under photo-irradiation and their efficacy against methicillin-resistant Staphylococcus aureus

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