Silver nanoparticles outperform gold nanoparticles in radiosensitizing U251 cells in vitro and in an intracranial mouse model of glioma

Liu, Pei Dang; Jin, Haizhen; Guo, Zhirui; Ma, Jun; Zhao, Jing; Li, Dongdong; Wu, Hao; Gu, Ning

doi:10.2147/ijn.s115473

Cited by 117 publications

(93 citation statements)

References 56 publications

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“…Similar results were also seen in NIH 3T3 mouse embryonic fibroblast cells, where nanosilver was observed to be contained in endosomes but not inside the nucleus, mitochondria, or golgi apparatus [63]. Nanosilver aggregates were observed in endosomes in U251 glioblastoma cells treated with citrate-coated nanosilver (15.26 nm) at a non-toxic concentration [64]. In vivo, examination of the livers of male Sprague Dawley rats treated with nanosilver (PVP-coated, 22.32 nm) via intraperitoneal injection revealed that the nanosilver was deposited in endosomes and lysosomes in Kupffer cells, and was generally in the liver cells closest to the blood vessels [65].…”

Section: Uptake Of Nanosilversupporting

confidence: 74%

A Current Overview of the Molecular Effects of Nanosilver

Cameron¹,

Willmore²

2018

Preprint

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Nanosilver plays an important role in nanoscience and nanotechnology, and is becoming increasingly used for applications in nanomedicine. Nanosilver ranges in size from one to one hundred nanometers. Smaller particles more readily enter cells and interact with the cellular components. The exposure dose, particle size, coating, and aggregation state of the nanosilver, as well as the cell type or organism that it is tested on, all have a large determining factor on the effect and potential toxicity of nanosilver. A high exposure dose to nanosilver alters the cellular stress responses and initiates cascades of signaling that can eventually trigger organelle autophagy and apoptosis. This review summarizes the current knowledge of the effects of nanosilver on cellular metabolic function and response to stress. Both the causative effects of nanosilver on oxidative stress, endoplasmic reticulum stress, and hypoxic stress, as well as the effects of nanosilver on the responses to such stresses, are outlined. The interactions and effects of nanosilver on cellular uptake, oxidative stress (reactive oxygen species), inflammation, hypoxic response, mitochondrial function, endoplasmic reticulum function and the unfolded protein response, autophagy and apoptosis, angiogenesis, epigenetics, genotoxicity, and cancer development and tumorigenesis, as well as other pathway alterations are examined in this review.

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Section: Uptake Of Nanosilversupporting

confidence: 74%

A Current Overview of the Molecular Effects of Nanosilver

Cameron¹,

Willmore²

2018

Preprint

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“…increase radiotherapy efficacy and prevent the development of drug-resistant tumors [44]. Another approach thoroughly studied by Rezaee, et al showed that electroporation enhances radiosensitizing effect of AuNPs in HT-29 colon adenocarcinoma cells as a result of increasing cell membrane permeability.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Cytotoxic and Antiproliferative Effects of Nanomaterials on Cancer Cell Lines: A Review

Grijalva¹,

Vallejo-López²,

Salazar³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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Cell models for the study of antiproliferative and/or cytotoxic properties of engineered nanoparticles are valuable tools in cancer research. Several techniques and methods are readily available for the study of nanoparticles' properties regarding selective toxicity and/or antiproliferative effects. Setting up of those techniques, however, needs to be carefully monitored. Harmonization of the wide range of methods available is necessary for assay comparison and replicability. Although individual or core laboratory capabilities play a role in selection and availability of techniques, data arising from cancer cell models are useful in guiding further research. The variety of cell lines available and the diversity of metabolic routes involved in cell responses make in vitro cell models suitable for the study of the biological effect of nanoparticles at the cell level and a valid approach for further in vivo and clinical studies. The present systematic review looks at the in vitro biological effects of different types of nanoparticles in cancer cell models.

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“…Nanoparticles (NP) have been used for therapy in diverse fields as a radiosensitiser [4], fluorescent labels [5], transfection vectors [6] and as drug carriers [7]. The size, shape and material composition confer advantageous properties on NP for various applications [8].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, metal nanoparticles have been utilised to enhance cytotoxicity in cancer cells using oxidising treatments such as radiation therapy [18,19]. Liu, et al, demonstrated AgNP outperforming gold nanoparticles (AuNP) in radiosensitising glioma cell line U251, where combination of AgNP and radiotherapy showed significant anti-glioma effect in vitro and in vivo in comparison to AuNP [4]. These studies resulted to multitude applications of employing NPs to cancer treatments through a vast number of strategies.…”

Section: Introductionmentioning

confidence: 99%

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

Manaloto

Gowen²,

Leśniak³

et al. 2020

Preprint

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Running title: Cold Atmospheric Plasma enhances silver nanoparticle uptake and cytotoxicity.2 Abstract Silver nanoparticles (AgNP) emerged as a promising reagent for cancer therapy with oxidative stress implicated in the toxicity. Meanwhile, studies reported cold atmospheric plasma (CAP) generation of reactive oxygen and nitrogen species has selectivity towards cancer cells. Gold nanoparticles display synergistic cytotoxicity when combined with CAP against cancer cells but there is a paucity of information using AgNP, prompting to investigate the combined effects of CAP using dielectric barrier discharge system (voltage of 75 kV, current is 62.5mA, duty cycle of 7.5kVA and input frequency of 50-60Hz) and 10nm PVA-coated AgNP using U373MG Glioblastoma Multiforme cells. Cytotoxicity in U373MG cells was >100-fold greater when treated with both CAP and PVA-AgNP compared with either therapy alone (IC 50 of 4.30 µg/mL with PVA-AgNP alone compared with 0.07 µg/mL after 25s CAP and 0.01 µg/mL 40s CAP). Combined cytotoxicity was ROS-dependent and was prevented using N-Acetyl Cysteine. A novel darkfield spectral imaging method investigated and quantified AgNP uptake in cells determining significantly enhanced uptake, aggregation and subcellular accumulation following CAP treatment, which was confirmed and quantified using atomic absorption spectroscopy. The results indicate that CAP decreases nanoparticle size, decreases surface charge distribution of AgNP and induces uptake, aggregation and enhanced cytotoxicity in vitro.

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Silver nanoparticles outperform gold nanoparticles in radiosensitizing U251 cells in vitro and in an intracranial mouse model of glioma

Cited by 117 publications

References 56 publications

A Current Overview of the Molecular Effects of Nanosilver

A Current Overview of the Molecular Effects of Nanosilver

Cytotoxic and Antiproliferative Effects of Nanomaterials on Cancer Cell Lines: A Review

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

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