Silver nanoparticles promote osteogenesis of mesenchymal stem cells and improve bone fracture healing in osteogenesis mechanism mouse model

Zhang, Ruizhong; Lee, Puiyan; Lui, Vincent Chi Hang; Chen, Yan; Liu, Xuelai; Lok, Chun Nam; To, Michael; Yeung, Kelvin Wai Kwok; Wong, Kenneth K.

doi:10.1016/j.nano.2015.07.016

Cited by 193 publications

(125 citation statements)

References 58 publications

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“…Another study, however, shows that Ag NP, which enter the cells through endocytosis and then accumulate in the cytosol, attenuate both osteogenic and adipogenic differentiation of hMSC, even at non-toxic concentrations [17]. On the contrary, Ag NP stimulate the osteogenic differentiation of murine MSC by activating TGF beta signaling [18]. …”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells

et al. 2017

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Infections of orthopedic implants are associated with high morbidity. The emergence of antibiotic resistant strains and the tendency of microbes to form biofilms on orthopedic devices prompt the individuation of novel antimicrobial agents. Silver nanoparticles represent an interesting alternative, but their effects on bone cells need to be clarified. We focused on osteoblast-like cells and on bone marrow-mesenchymal stem cells and found that these cells are rather resistant to the cytotoxic effects of silver nanoparticles, with a half maximal inhibitory concentration around 25 µg/mL as detected by MTT assay. Within a month of treatment, osteoblast-like cells adapt to the presence of the nanoparticles by upregulating hsp70 as shown by western blot. Hsp70 overexpression correlates with the restoration of normal cell proliferation. No alterations in the extent and time requirements were detected in mesenchymal stem cell induced to differentiate in osteoblasts in the presence of silver nanoparticles. Because the concentrations of silver nanoparticles which show antimicrobial activity are lower than those exerting toxic effects on bone-forming cells in vitro, we suggest that silver nanoparticles might represent a challenging tool to fight infections in orthopedic implants.

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Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells

et al. 2017

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“…Hsa-miR-34a-3p, hsa-miR-145-3p, hsa-miR-301a-3p, hsa-miR-323a-3p, hsa-miR-331-5p and hsa-miR-424-3p were predicted to regulate the TGF-β/bone morphogenetic protein (BMP) signaling pathway. The TGF-β/BMP signaling pathway has been reported to be associated with osteogenic differentiation of MSCs (31). Hsa-miR-34a-3p, hsa-miR-7-5p and hsa-miR-323a-3p were predicted to regulate the fibroblast growth factor signaling pathway, which has been reported to be important for differentiation, growth and proliferation of MSCs (32,33).…”

Section: Discussionmentioning

confidence: 99%

Interferon-γ alters the microRNA profile of umbilical cord-derived mesenchymal stem cells

Cheng

Cui

Wang

et al. 2016

Molecular Medicine Reports

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Numerous studies have demonstrated that interferon-γ (IFN-γ) is an important inflammatory cytokine, which may activate the immunomodulatory abilities of mesenchymal stem cells (MSCs), and may influence certain other functions of these cells. MicroRNAs are small non-coding RNAs that regulate the majority of the biological functions of cells and are important in a variety of biological processes. However, few studies have been performed to investigate whether IFN-γ affects the microRNA profile of MSCs. The aim of the present study was to analyze the microRNA profile of MSCs derived from the umbilical cord (UC-MSCs) cultured in the presence or absence of IFN-γ (IFN-UC-MSCs). An array that detects 754 microRNAs was used to determine the expression profiles. Statistical analysis of the array data revealed that 8 microRNAs were significantly differentially expressed in UC-MSCs and IFN-UC-MSCs. Reverse transcription-quantitative polymerase chain reaction validated the differential expression of the 8 identified microRNAs. The target genes of the 8 microRNAs were predicted through two online databases, TargetScan and miRanda, and the predicted results were screened by bioinformatics analysis. The majority of the target genes were involved in the regulation of transcription, signal transduction, proliferation, differentiation and migration. These results may provide insight into the mechanism underlying the regulation of the biological functions of MSCs by IFN-γ, in particular the immunomodulatory activity.

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“…The potent and broad‐spectrum antimicrobial activity of AgNPs has been exploited for manufacturing medical devices and for tissue engineering. AgNPs showed osteoinductivity in mouse MSCs through enhancing proliferation and osteogenic differentiation of MSCs, demonstrated by induction of ALP activity .…”

Section: Nanoparticles and Stem Cell Differentiationmentioning

confidence: 99%

The potential of nanoparticles in stem cell differentiation and further therapeutic applications

Dayem

Choi

Yang

et al. 2016

Biotechnology Journal

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Tissue regeneration could offer therapeutic advantages for individuals experiencing organ or tissue damage. Recently, advances in nanotechnology have provided various nanomaterials, with a wide range of applications, for modulating stem cell behavior and for further therapeutic applications in tissue regeneration. Defects in cell proliferation and differentiation, a low mechanical strength of scaffolds, and inefficient production of factors that are essential for stem cell differentiation are the current challenges in tissue regeneration. This review provides a brief explanation about the link between nanotechnology and tissue engineering, highlighting the current literature about the interaction between nanoparticles (NPs) and stem cells, the promotional effect of NPs on stem cell differentiation into various lineages, and their possible therapeutic applications. We also tried to describe the mechanism through which NPs regulate the spatial-temporal release and kinetics of vital growth and differentiation factors, enhance stem cell differentiation, and improve culture conditions for in vivo tissue regeneration. The field of nanotechnology is promising and provides novel nanomaterials and methods with valuable clinical applications in the regenerative medicine. Understanding the mechanism, as well as the toxic effects of NPs in stem cell biology will undoubtedly provide valuable insight into their clinical application in the regenerative medicine.

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Silver nanoparticles promote osteogenesis of mesenchymal stem cells and improve bone fracture healing in osteogenesis mechanism mouse model

Cited by 193 publications

References 58 publications

Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells

Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells

Interferon-γ alters the microRNA profile of umbilical cord-derived mesenchymal stem cells

The potential of nanoparticles in stem cell differentiation and further therapeutic applications

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