Silver nanoparticles rapidly induce atypical human neutrophil cell death by a process involving inflammatory caspases and reactive oxygen species and induce neutrophil extracellular traps release upon cell adhesion

Liz, Rafael de; Simard, Jean-Christophe; Leonardi, Laurien Bruna Araújo; Girard, Denis

doi:10.1016/j.intimp.2015.06.030

Cited by 67 publications

(52 citation statements)

References 49 publications

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“…The size of the nanoparticles allows them to easily enter the cells of living organisms, which can lead to several cell lesions 49,50. Concerns on the potential cytotoxicity and genotoxicity of nanoparticles has increased significantly; therefore, these have been intensively studied 51–53…”

Section: Resultsmentioning

confidence: 99%

Antibacterial effect of silver nanoparticles in <em>Pseudomonas aeruginosa</em>

Salomoni¹,

Léo²,

Montemor³

et al. 2017

NSA

267

122

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Pseudomonas aeruginosa has great intrinsic antimicrobial resistance limiting the number of effective antibiotics. Thus, other antimicrobial agents such as silver nanoparticles (AgNPs) are considered potential agents to help manage and prevent infections. AgNPs can be used in several applications against bacteria resistant to common antibiotics or even multi-resistant bacteria such as P. aeruginosa. This study assessed the antimicrobial activity of commercial 10 nm AgNPs on two hospital strains of P. aeruginosa resistant to a large number of antibiotics and a reference strain from a culture collection. All strains were susceptible to 5 µg/mL nanoparticles solution. Reference strains INCQS 0230 and P.a.1 were sensitive to AgNPs at concentrations of 1.25 and 0.156 µg/mL, respectively; however, this was not observed for hospital strain P.a.2, which was more resistant to all antibiotics and AgNPs tested. Cytotoxicity evaluation indicated that AgNPs, up to a concentration of 2.5 µg/mL, are very safe for all cell lines tested. At 5.0 µg/mL, AgNPs had a discrete cytotoxic effect on tumor cells HeLa and HepG2. Results showed the potential of using AgNPs as an alternative to conventional antimicrobial agents that are currently used, and a perspective for application of nanosilver with antibiotics to enhance antimicrobial activity.

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Section: Resultsmentioning

confidence: 99%

Antibacterial effect of silver nanoparticles in <em>Pseudomonas aeruginosa</em>

Salomoni¹,

Léo²,

Montemor³

et al. 2017

NSA

267

122

View full text Add to dashboard Cite

show abstract

“…It causes disturbance of its power function, disintegration of membrane structure and release of cell contents [9, 44–46]. It has been reported that antimicrobial activity of silver nanoparticles is related to the formation of free oxygen radicals, which induce cell membrane damage [47].…”

Section: Discussionmentioning

confidence: 99%

“…Nanometer size of nanoparticles allows them to easily enter to cells of living organisms, leading to various cell injuries [8, 9]. The potential cytotoxicity and genotoxicity of nanoparticles have risen significantly and have been studied intensively [10, 11].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles

Składanowski

Golińska

Rudnicka

et al. 2016

Med Microbiol Immunol

109

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The study was focused on assessment of antibacterial activity, cytotoxicity and immune compatibility of biogenic silver nanoparticles (AgNPs) synthesized from Streptomyces sp. NH28 strain. Nanoparticles were biosynthesized and characterized by UV–Vis spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, nanoparticle tracking analysis system and zeta potential. Antibacterial activity was tested against Gram-positive and Gram-negative bacteria; minimal inhibitory concentration was recorded. Cytotoxicity was estimated using L929 mouse fibroblasts via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Biocompatibility of AgNPs was performed using THP1-XBlue™ cells. Biogenic AgNPs presented high antibacterial activity against all tested bacteria. Minimum inhibitory concentration of AgNPs against bacterial cells was found to be in range of 1.25–10 μg/mL. Silver nanoparticles did not show any harmful interaction to mouse fibroblast cell line, and no activation of nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) cells was observed at concentration below 10 µg/mL. The half-maximal inhibitory concentration (IC50) value was established at 64.5 μg/mL. Biological synthesis of silver can be used as an effective system for formation of metal nanoparticles. Biosynthesized AgNPs can be used as an antibacterial agent, which can be safe for eukaryotic cells.

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“…Upon stimulation by NPs (TiO2—titanium dioxide—and alum), Duffin et al (58) demonstrated neutrophil influx to the lungs and also induced production of IL-18. Silver NPs were also shown to be capable of interacting with neutrophils, inducing apoptosis of these cells, and inducing caspase-1\caspase-4 partially dependent IL-1β secretion (59). In another study, cobalt and nickel NPs were shown to induce higher nitric oxide, TNF-α, and CXCL2 chemokine production, by human peripheral blood neutrophils, than titanium NPs (TiO2NP) (60).…”

Section: Nps As Adjuvants To Generate Th1 and Th17 Responsesmentioning

confidence: 99%

Role of Metallic Nanoparticles in Vaccinology: Implications for Infectious Disease Vaccine Development

2017

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Subunit vaccines are safer but less immunogenic than live-attenuated vaccines or whole cell inactivated vaccines. Adjuvants are used to enhance and modulate antigen (Ag) immunogenicity, aiming to induce a protective and long-lasting immune response. Several molecules and formulations have been studied for their adjuvanticity, but only seven have been approved to formulate human vaccines. Metallic nanoparticles (MeNPs), particularly those containing gold and iron oxides, are widely used in medicine for diagnosis and therapy and have been used as carriers for drugs and vaccines. However, little is known about the immune response elicited by MeNPs or about their importance in the development of new vaccines. There is evidence that these particles display adjuvant characteristics, promoting cell recruitment, antigen-presenting cell activation, cytokine production, and inducing a humoral immune response. This review focuses on the characteristics of MeNPs that could facilitate the induction of a cellular immune response, particularly T-helper 1 and T-helper 17, and their potential functions as adjuvants for subunit vaccines.

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Silver nanoparticles rapidly induce atypical human neutrophil cell death by a process involving inflammatory caspases and reactive oxygen species and induce neutrophil extracellular traps release upon cell adhesion

Cited by 67 publications

References 49 publications

Antibacterial effect of silver nanoparticles in <em>Pseudomonas aeruginosa</em>

Antibacterial effect of silver nanoparticles in <em>Pseudomonas aeruginosa</em>

Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles

Role of Metallic Nanoparticles in Vaccinology: Implications for Infectious Disease Vaccine Development

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