Silver nitrate promotes high-frequency multiple shoot regeneration in cotton (Gossypium hirsutum L.) by inhibiting ethylene production and phenolic secretion

Kumar, G. Prem; Shanmugam, Sivakumar; Siva, G.; Vigneswaran, M.; Kumar, T. Senthil; Jayabalan, N.

doi:10.1007/s11627-016-9782-5

Cited by 33 publications

(4 citation statements)

References 64 publications

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“…Silver ions in AgNO3 can interfere with ethylene signaling by blocking ethylene receptors, thus inhibiting the plant's response to ethylene [28][29][30][31]. Furthermore, silver nitrate can reduce ethylene production by inhibiting aminocyclopropane-1-carboxylic acid [32], a key component of the ethylene biosynthesis pathway, while promoting cell division and growth through the stimulation of polyamines [32][33][34]. Different concentrations of AgNO3 have varying effects on plant growth parameters.…”

Section: Discussionmentioning

confidence: 99%

Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization

Kovács,

Portocarrero,

Honfi

et al. 2024

Plants

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The endangered plant species Adenophora liliifolia faces threats to its survival in the wild, necessitating the development of effective micropropagation techniques for potential reintroduction efforts. This study demonstrates that Adenophora liliifolia effectively reproduces on MS synthetic medium with diverse plant growth regulators (PGR) and natural extracts, facilitating swift micropropagation for potential future reintroduction endeavors. It highlights the substantial impact of PGR composition and natural extracts on the growth and development of A. liliifolia. The ideal growth medium for A. liliifolia was determined to be ½ MS with specific treatments. Additionally, incorporating silver nitrate (AgNO3) at 5 mg L−1 into the medium led to enhanced root formation and shoot length, albeit excessive concentrations adversely affected root development. Varying concentrations of NAA significantly affected different plant growth parameters, with the 0.1 mg L−1 treatment yielding comparable plant height to the control. Moreover, 50 mL L−1 of coconut water bolstered root formation, while 200 mL L−1 increased shoot formation during in vitro propagation. However, elevated doses of coconut water (CW) impeded root development but stimulated shoot growth. Experiments measuring chlorophyll a + b and carotenoid content indicated higher concentrations in the control group than differing levels of applied coconut water. Optimizing pH levels from 6.8–7 to 7.8–8.0 notably enhanced plant height and root formation, with significant carotenoid accumulation observed at pH 6.8–7. Soil samples from A. liliifolia’s natural habitat exhibited a pH of 6.65. Ultimately, the refined in vitro propagation protocol effectively propagated A. liliifolia, representing a pioneering effort and setting the stage for future restoration initiatives and conservation endeavors.

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Section: Discussionmentioning

confidence: 99%

Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization

Kovács,

Portocarrero,

Honfi

et al. 2024

Plants

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“…The lowest values of EC% rate, CI% rate and RE number were obtained from MS medium with 4 mg L −1 AgNO 3 . The gaseous ingredients inside the tissue culture system, with a particular emphasis on ethylene, have a significant influence on the growth and developmental processes of plants [ 2 , 6 ]. According to the literature, the presence of ethylene has been shown to hinder the process of somatic embryogenesis and the production of shoot primordia in callus cultures [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

“…Ethylene, a plant growth regulator, is known to influence morphogenesis in plant culture [ 2 , 3 , 4 ]. Prior research has shown that ethylene has the potential to hinder shoot rejuvenation, callus growth, and somatic embryogenesis [ 5 , 6 ]. Closed vessels are utilized in tissue culture to prevent contamination.…”

Section: Introductionmentioning

confidence: 99%

Machine Learning Analysis of the Impact of Silver Nitrate and Silver Nanoparticles on Wheat (Triticum aestivum L.): Callus Induction, Plant Regeneration, and DNA Methylation

Türkoğlu,

Haliloğlu,

Demirel

et al. 2023

Plants

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The objective of this study was to comprehend the efficiency of wheat regeneration, callus induction, and DNA methylation through the application of mathematical frameworks and artificial intelligence (AI)-based models. This research aimed to explore the impact of treatments with AgNO3 and Ag-NPs on various parameters. The study specifically concentrated on analyzing RAPD profiles and modeling regeneration parameters. The treatments and molecular findings served as input variables in the modeling process. It included the use of AgNO3 and Ag-NPs at different concentrations (0, 2, 4, 6, and 8 mg L−1). The in vitro and epigenetic characteristics were analyzed using several machine learning (ML) methods, including support vector machine (SVM), random forest (RF), extreme gradient boosting (XGBoost), k-nearest neighbor classifier (KNN), and Gaussian processes classifier (GP) methods. This study’s results revealed that the highest values for callus induction (CI%) and embryogenic callus induction (EC%) occurred at a concentration of 2 mg L−1 of Ag-NPs. Additionally, the regeneration efficiency (RE) parameter reached its peak at a concentration of 8 mg L−1 of AgNO3. Taking an epigenetic approach, AgNO3 at a concentration of 2 mg L−1 demonstrated the highest levels of genomic template stability (GTS), at 79.3%. There was a positive correlation seen between increased levels of AgNO3 and DNA hypermethylation. Conversely, elevated levels of Ag-NPs were associated with DNA hypomethylation. The models were used to estimate the relationships between the input elements, including treatments, concentration, GTS rates, and Msp I and Hpa II polymorphism, and the in vitro output parameters. The findings suggested that the XGBoost model exhibited superior performance scores for callus induction (CI), as evidenced by an R2 score of 51.5%, which explained the variances. Additionally, the RF model explained 71.9% of the total variance and showed superior efficacy in terms of EC%. Furthermore, the GP model, which provided the most robust statistics for RE, yielded an R2 value of 52.5%, signifying its ability to account for a substantial portion of the total variance present in the data. This study exemplifies the application of various machine learning models in the cultivation of mature wheat embryos under the influence of treatments and concentrations involving AgNO3 and Ag-NPs.

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“…Another report published by Tamimi (2015), showed that AgNO 3 produced maximum shoot length, leaf surface area and leaf chlorophyll content (120%) in the Musa acuminata L. Additionally, AgNO 3 decrease amount of ACC and S-adenosylmethionine synthetase activity as key precursors ethylene and polyamines production. It need to be keep in mind that, polyamines increase plant growth and cell proliferation under salt stress in tomato plants (Prem Kumar et al, 2016). So, AgNO 3 may increase salt tolerance by polyamine production in plant.…”

Section: Discussionmentioning

confidence: 99%

Ethylene inhibition with silver nitrate (AgNO3) and pyrazinamide (PZA) ameliorates in vitro salt tolerance of tomato (Lycopersicon esculentum L) plantlets

Zarei

Ehsanpour

2023

Plant Cell Tiss Organ Cult

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The purpose of this research was to nd out how in vitro culture of tomato explants respond to salt stress by silver nitrate (AgNO 3 ) and pyrazinamide (PZA) as ethylene inhibitors. Tomato seedlings were grown on MS medium containing NaCl (0, 100, 150 mM) and supplemented with AgNO 3 (0, 2, 4, mg. L -1 ) and pyrazinamide (PZA) (0, 2, 4,mg. L -1 ) to study growth parameters and antioxidant enzymes responses. Salt-stressed plants showed limited growth and a signi cant decrease in fresh and dry weight. Salinity accelerated oxidative damage by increasing hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) levels in tomato leaves. In the present of AgNO 3 and PZA, in vitro grown tomato plants increased fresh and dry weight total chlorophyll, carotenoid in particular at 4 mg. L -1 AgNO 3 and 4 mg. L -1 PZA. Moreover, silver nitrate and PZA reduced H 2 O 2 and MDA contents and oxidative damage by enhancing antioxidant enzyme activity, including catalase, superoxide dismutase, and ascorbate peroxidase under salt stress. According to our ndings, AgNO 3 and PZA improved in vitro salinity tolerance of tomato plant by interfering ethylene action or ethylene generation and increasing biochemical responses.

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Silver nitrate promotes high-frequency multiple shoot regeneration in cotton (Gossypium hirsutum L.) by inhibiting ethylene production and phenolic secretion

Cited by 33 publications

References 64 publications

Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization

Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization

Machine Learning Analysis of the Impact of Silver Nitrate and Silver Nanoparticles on Wheat (Triticum aestivum L.): Callus Induction, Plant Regeneration, and DNA Methylation

Ethylene inhibition with silver nitrate (AgNO3) and pyrazinamide (PZA) ameliorates in vitro salt tolerance of tomato (Lycopersicon esculentum L) plantlets

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