HighlightsWe have evaluated the role of carbon sources in controlling excessive phenolic secretion from callus culture of cotton.Maltose was found to control phenolic secretion when it is used as a sole carbon source.Efficient protocol was developed for high frequency callus induction and proliferation for SVPR-2 cotton cultivar using different plant growth regulators.Adjustment of carbon source concentrations was found to play an important role in cotton callus culture.The present work will be helpful for establishing callus culture and getting regeneration to carry out genetic transformation studies in SVPR-2 cotton cultivar.

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