1988
DOI: 10.3109/10520298809107156
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Silver Staining of the Nucleolar Organizer Regions (NORS) in Semithin Lowicryl Sections

Abstract: The one-step silver technique was applied to semithin Lowicryl sections of root meristem cells of Allium cepa and a human tumor cell line (TG cells). In vegetal cells, after 5 min of staining reaction, the Ag-NOR proteins formed ring-shaped structures peripherally within the nucleolus. In animal cells silver granules were distributed over the entire nucleolus. The specificity of the staining reaction was increased by incubation of the sections in NH4Cl and Schiff's reagent prior to Ag-NOR silver staining.

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Cited by 13 publications
(12 citation statements)
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“…NOR-silver staining has been described as a cytochemical method for light and electron microscopy (Foucrier et al, 1990;Rowlands et al, 1990;Hubbell, 1985;Moreno et al. 1985;Thiebaut et al.…”
Section: Discussionmentioning
confidence: 99%
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“…NOR-silver staining has been described as a cytochemical method for light and electron microscopy (Foucrier et al, 1990;Rowlands et al, 1990;Hubbell, 1985;Moreno et al. 1985;Thiebaut et al.…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, to overcome these difficulties, pre-treatment with Schiffs reagent (Thiebaut et al, 1984), glycine (Cromie et al, 1988), or ammonium chloride (Moreno et al, 1985) has been recommended. High levels of nonspecific staining on ultra-thin sections have also been found when phosphate buffer was used (Moreno et al, 1985). We therefore tested the effect of different fixatives on the efficiency of the one-step silver reaction with gelatin developer.…”
Section: Discussionmentioning
confidence: 99%
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“…The one step method (Howell & Black, 1980), is an ingenious modification of the more time consuming original three step technique and has itself undergone modification including preincubation with glycine to reduce incubation time (Cromie et al, 1988), the use of polyethylene glyco as a protective colloidal developer (in place of gelatin), to reduce non-specific deposit (Rowlands et al, 1990), and the inclusion of a celloidin film, also to reduce nonspecific (Chiu et al, 1989). Furthermore, minor adaptations have been employed to allow application to cell imprints Ruschoff et al, 1989), cytospin preparations , and semithin methacrylate sections (Moreno et al, 1989).…”
Section: Physiology and Theoretical Basismentioning
confidence: 99%