Silymarin attenuates the renal ischemia/reperfusion injury-induced morphological changes in the rat kidney

Şentürk, Hakan; Kabay, Şahin; Bayramoğlu, Gökhan; Özden, Hilmi; Yaylak, Faik; Yücel, Mehmet; Olgun, Esra Gurlek; Kutlu, Ali

doi:10.1007/s00345-008-0256-1

Cited by 37 publications

(24 citation statements)

References 23 publications

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“…[20] Our results confirm that renal I/R enhances oxidative stress by modulating production of free radicals and reactive oxygen species, and also modulating the production of toxic cytokines, leading to inflammation and leukocyte infiltration or direct tissue damage, as shown in previous studies. [21,22] In this study, we also investigated the effects of NS on the parameters implicating oxidative and antioxidative status. We observed significantly decreased TOS and OSI levels and increased TAC levels with the administration of NS intraperitoneally, which could be partly related to the antioxidant and free-radical scavenging effects of NS.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Nigella sativa against Ischemia-Reperfusion Injury of Kidneys

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Nigella sativa against Ischemia-Reperfusion Injury of Kidneys

et al. 2010

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“…2,3,18,19,41 The discrepant effect on oxidative stress suggests that heme-catalyzed reactive species generation may differ from reactive species generated in other experimental conditions. Alternatively, silymarin-induced p53 activation could upregulate genes that encode ROS-generating enzymes (PIG 3, PIG 6) 42 or suppress antioxidant genes (MnSOD), 43 either way tipping the balance toward oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…This AKI model (Gly-AKI) is characterized by renal ischemia and myoglobin-derived heme-iron-mediated renal oxidative stress [10][11][12][13][14] leading to severe tubular injury (necrosis and apoptosis), renal and systemic inflammatory response, preglomerular vasoconstriction, and acute renal failure. [15][16][17] Based on the previous studies in rats subjected to renal ischemia/reperfusion injury, 18,19 we expected that silymarin would have antioxidant and protective effect in Gly-AKI rats. The initial purpose of this experimental study was to evaluate the potential of this herbal extract in the renal protection after rhabdomyolysis.…”

Section: Introductionmentioning

confidence: 99%

Silymarin exacerbates p53-mediated tubular apoptosis in glycerol-induced acute kidney injury in rats

2010

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Background/aims: Silymarin is an herbal extract with antioxidant properties that can reduce oxidative stressmediated injuries in murine models of liver, heart, and kidney diseases. Silymarin can also increase p53-mediated cellular apoptosis in vitro. We tested the effect of silymarin administration before glycerol-induced acute kidney injury (Gly-AKI) in rats. Methods: Renal function, tubular injury, oxidative stress, leukocytes infiltration, and renal expression of apoptosis regulating proteins (p53, p-p53, Bax, Bcl-2, survivin, and cleaved caspase-3) were evaluated 6 or 24 h after glycerol. Results: Silymarin exacerbated the renal impairment and tubular apoptosis but had no effect on tubular necrosis or renal leukocytes infiltration. Renal lipid and DNA peroxidation was increased after glycerol and silymarin did not reduce oxidative stress. Proteins p53, p-p53, and proapoptotic Bax were upregulated in Gly-AKI rats treated with silymarin, whereas anti-apoptotic Bcl-2 was reduced in this group. Cleaved caspase-3 was overexpressed in Gly-AKI rats, particularly when treated with silymarin. Survivin was less expressed in Gly-AKI than in controls, but this deficit was not aggravated by silymarin. Conclusion: The persistence of oxidative stress, inflammatory reaction, and tubular necrosis, as well as exacerbation of p53-mediated tubular apoptosis, led to a more severe renal impairment in Gly-AKI rats treated with silymarin.

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“…The favorable effect of silibinin may be masked by the presence of ethanol effect and this can be avoided by the use of water soluble form of silibinin in further studies. Thus, several lines of evidence show its beneficial effects in IRI of distinct origin [5][6][7][8] .…”

Section: Resultsmentioning

confidence: 99%

“…With its anti-oxidant and anti-inflammatory properties, silibinin (also known as silybin) constitutes the most important part of this extract 4 . Thus, silymarin and/or silibinin have been found to exert favorable effects in various IRI models of different tissues, including liver, brain, kidney, and heart [5][6][7][8][9][10] . However, silibinin has not been explored in skeletal muscle IRI.…”

Section: Introductionmentioning

confidence: 99%

Effects of silibinin and ethanol on skeletal muscle ischemia-reperfusion injury

et al. 2013

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PURPOSE:To investigate the potential beneficial effect of silibinin in ischemia-reperfusion injury (IRI) of skeletal muscle. METHODS: Under urethane anesthesia, four experimental groups were established in Balb/c mice: I) Sham-control, II) IRI (Tourniquet-induced) (2+1 h), III) IRI+ethanol (10%), and IV) IRI+silibinin (50 mg/kg/IP). The viability of muscle (left) was evaluated by the triphenyltetrazolium chloride dye method and calculated as the percentage of the contralateral control muscle (right). Malondialdehyde, superoxide dismutase, and catalase were measured in the gastrocnemius muscle via a spectrophotometer. RESULTS:The viability of gastrocnemius muscle in group II was significantly lower in comparison with that seen in group I. The administration of either ethanol or silibinin rendered the tissues to recover nearly to the baseline level. Additionally, malondialdehyde levels were higher in group II than those in group I. The application of silibinin prior to the reperfusion attenuated these to the control levels. However, malondialdehyde levels in the ethanol administrated group were reduced as well. The enhanced superoxide dismutase activity seen in the IRI group was not diminished in the animals treated with either silibinin or ethanol. Similarly, there were no differences between groups regarding the catalase activities. CONCLUSION: Ethanol seems to be effective in attenuating IRI in skeletal muscle and no definite conclusion can be made on silibinin effect.

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Silymarin attenuates the renal ischemia/reperfusion injury-induced morphological changes in the rat kidney

Cited by 37 publications

References 23 publications

Protective Effects of Nigella sativa against Ischemia-Reperfusion Injury of Kidneys

Protective Effects of Nigella sativa against Ischemia-Reperfusion Injury of Kidneys

Silymarin exacerbates p53-mediated tubular apoptosis in glycerol-induced acute kidney injury in rats

Effects of silibinin and ethanol on skeletal muscle ischemia-reperfusion injury

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