Silymarin modulates doxorubicin-induced oxidative stress, Bcl-xL and p53 expression while preventing apoptotic and necrotic cell death in the liver

Patel, Nirav; Joseph, Cecil K.; Corcoran, George B.; Ray, Sidhartha D.

doi:10.1016/j.taap.2010.02.002

Cited by 151 publications

(125 citation statements)

References 98 publications

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“…Apoptosis is an important mechanism regulating cell number and their development in different organs and tissues and is a common feature of hepatotoxicity induced by chemicals or drugs. Doxorubicin is known to kill cells primarily by fragmenting DNA and causing cell death by apoptosis (Ray and Jena, 2000;Patel et al, 2010). In the present study, immunohistochemistry for caspase-3 expression in mice livers indicated that DOX induced apoptosis in hepatocytes.…”

Section: Discussionsupporting

confidence: 57%

Protective effect of Moringa peregrina seed oil against doxorubicin-induced hepatotoxicity in male mice

2016

Res. Opin. Anim. Vet. Sci.

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The present study aimed to illustrate the histopathological and immunohistochemical changes in the liver of mice exposed to anticancer drug, doxorubicin (DOX), and to evaluate the possible protective role of Moringa peregrina seed oil. Thirty two mature male mice were randomly divided into four groups. One group served as a control, the other was given a dose of M. peregrine at the rate of 150 mg/kg body weight, third group was DOX treated, and fourth was DOX plus M. peregrina treated. Histopathological examination of liver sections showed that DOX caused disturbance in hepatic cords, hepatocytes degeneration, inflammation, necrotic areas, vacuolation and dilation of the blood sinusoids. Doxorubicin treated animals demonstrated a positive reaction to Caspase-3 in hepatocytes as compared with controls. Administration of M. peregrina seed oil plus doxorubicin showed reduction in caspase-3 immunoreactivity and apoptotic index induced by DOX. Treatment with M. peregrina seed oil 150 mg/kg body weight reduced the damage caused by DOX in the liver. Keywords: Doxorubicin; Moringa seed oil; histopathology; liver; mice To cite this article: Sliai AM and GH Abdel-Rahman, 2016. Protective effect of Moringa peregrina seed oil against doxorubicin-induced hepatotoxicity in male mice. Res. Opin. Anim. Vet. Sci., 6(2): 69-73.

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Section: Discussionsupporting

confidence: 57%

Protective effect of Moringa peregrina seed oil against doxorubicin-induced hepatotoxicity in male mice

2016

Res. Opin. Anim. Vet. Sci.

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“…Similar to other cancer chemotherapeutic agents, doxorubicin induces apoptosis both in vivo [19][20][21][22] and in vitro. 2,8,9 To determine whether the pro-apoptotic effects of doxorubicin are regulated through activation of ZAK, we examined the cleavage of poly (ADP-ribose) polymerase (PARP) (from 116 kDa to 89 kDa) and caspase-3 (from procaspase to p20/p19 and p17 subunits), which are frequently employed as indicators of apoptosis.…”

Section: Doxorubicinmentioning

confidence: 99%

ZAK is required for doxorubicin, a novel ribotoxic stressor, to induce SAPK activation and apoptosis in HaCaT cells

Sauter

Magun

Iordanov

et al. 2010

Cancer Biology & Therapy

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“…The net result of these events is the accumulation of a variety of toxic lipid peroxides and MDA. The level of liver tissue MDA is reported to be a reliable marker of lipid peroxidation (Patel et al, 2010). Therefore; the hepatoprotective activity exhibited by N. S extract might be due to the antioxidative nature of the plant.…”

Section: Discussionmentioning

confidence: 99%

Nigella sativa and Aloe vera protection against doxorubicin hepatotoxicity

Elzawahry¹,

Kholoussi²,

Raouf³

et al. 2016

J. Exper. Appl. Anim. Sci.

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Doxorubicin (DOX) is a potent available antitumor agent; however, its clinical use is limited due to its significant toxicity to most tissues and organs. Administration of DOX induced acute hepatotoxicity manifested by significant elevation in serum and liver lipid peroxidation. The present study was designed to evaluate and confirm the protective effect of Nigella sativa (N.S), Aloe vera (A.V) and their mixture. A cumulative dose (30 mg/kg i.p.) of DOX divided into 6 equal doses administered over six weeks to male albino rats separate or combined with N.S, A.V or their mixture extract. At the end of the experiment, biochemical and histological parameters were assessed. Concerning oxidative stress and antioxidant defense system, the depleted hepatic glutathione content (GSH) of DOX-administered rats as well as glutathione peroxidase (GPx), catalase (CAT) and super oxide dismutase (SOD) activities were increased above normal levels as a result of pretreatment with N.S, A.V and their mixture. However, while elevated lipid peroxidation was noticed in DOX treated rats, pretreatment with N.S, A.V and their mixture produced a detectable decrease in the lipid peroxidation level. Histological measurements showed that DOX caused a marked rise multiple focal coagulative necrosis of hepatocytes associated with hemorrhage while rats pretreated with N.S, A.V and their mixture in combination with DOX showed no histopathological changes compared to normal control. From these data, it can be concluded that N.S, A.V and their mixture of extracts could be protective for the liver cells against DOX-induced hepatotoxicity due to their contents of polyphenolic compounds that might serve as novel adjuvant therapy with DOX.

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Silymarin modulates doxorubicin-induced oxidative stress, Bcl-xL and p53 expression while preventing apoptotic and necrotic cell death in the liver

Cited by 151 publications

References 98 publications

Protective effect of Moringa peregrina seed oil against doxorubicin-induced hepatotoxicity in male mice

Protective effect of Moringa peregrina seed oil against doxorubicin-induced hepatotoxicity in male mice

ZAK is required for doxorubicin, a novel ribotoxic stressor, to induce SAPK activation and apoptosis in HaCaT cells

Nigella sativa and Aloe vera protection against doxorubicin hepatotoxicity

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