Similar Gut Bacterial Microbiota in Two Fruit-Feeding Moth Pests Collected from Different Host Species and Locations

Gong, Qiang; Cao, Li‐Jun; Sun, Lina; Chen, Jin-Cui; Gong, Ya‐Jun; Pu, De-Qiang; Huang, Qiong; Hoffmann, Ary A.; Wei, Shu‐Jun

doi:10.3390/insects11120840

Cited by 12 publications

(16 citation statements)

References 68 publications

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“…At the phylum level, there were no significant differences in the bacterial composition of G. molesta across the five diets of apples, peaches, nectarines, crisp pears, and peach shoots, but the diversity of gut bacteria was significantly lower than that of the artificial feed and plums. In these five treatments, Proteobacteria was the absolute dominant phylum, and its relative abundances were more than 90%, similar to previous researches [20,31,33]. However, in the two treatment groups that fed on an artificial feed and plums, the following four phyla were dominant: Proteobacteria, Firmicutes, Bacteroidota, and Actinobacteriota.…”

Section: Discussionsupporting

confidence: 87%

“…The larvae were selected and surface-sterilized in 75% ethanol for 60 s, followed by 3 washes in sterile water for 30 s. Midgut tissues were dissected with sterile forceps in a sterile petri dish with a diameter of 90 mm and rinsed again in sterile phosphate buffered saline (PBS) [7,20,31,33]. Fifty midguts were collected per sample using a sterile centrifuge tube.…”

Section: Dna Extractionmentioning

confidence: 99%

“…Yang et al revealed that the diversity of gut microbes in Plutella xylostella was significantly decreased after the host shifted from radishes to peas and was raised for 17 generations [7]. There have been some studies on the gut microbiota of G. molesta [20,31,33], but as far as we know, there are few reports about the structure of the gut bacterial communities when G. molesta feed on the fruits or young shoots of Rosaceae hosts.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Gut Bacterial Communities of Grapholita molesta (Lepidoptera: Tortricidae) Reared on Different Host Plants

Yuan

Zhang

Liu

et al. 2021

IJMS

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Intestinal symbiotic bacteria have played an important role in the digestion, immunity detoxification, mating, and reproduction of insects during long-term coevolution. The oriental fruit moth, Grapholita molesta, is an important fruit tree pest worldwide. However, the composition of the G. molesta microbial community, especially of the gut microbiome, remains unclear. To explore the differences of gut microbiota of G. molesta when reared on different host plants, we determined the gut bacterial structure when G. molesta was transferred from an artificial diet to different host plants (apples, peaches, nectarines, crisp pears, plums, peach shoots) by amplicon sequencing technology. The results showed that Proteobacteria and Firmicutes are dominant in the gut microbiota of G. molesta. Plum-feeding G. molesta had the highest richness and diversity of gut microbiota, while apple-feeding G. molesta had the lowest. PCoA and PERMANOVA analysis revealed that there were significant differences in the gut microbiota structure of G. molesta on different diets. PICRUSt2 analysis indicated that most of the functional prediction pathways were concentrated in metabolic and cellular processes. Our results confirmed that gut bacterial communities of G. molesta can be influenced by host diets and may play an important role in host adaptation.

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Section: Discussionsupporting

confidence: 87%

Section: Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Gut Bacterial Communities of Grapholita molesta (Lepidoptera: Tortricidae) Reared on Different Host Plants

Yuan

Zhang

Liu

et al. 2021

IJMS

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“…Taxonomic analysis revealed that the wild ghost moth gut bacterial community was dominated by Proteobacteria and Firmicutes (>99%), while the laboratory-reared ghost moth gut bacterial community was mainly composed of Proteobacteria with significantly decreased abundance in Firmicutes, but increased abundance in Bacteroidetes and Actinobacteria ( Figure 1 ). These four phyla were also the most common found in other lepidopteran species [ 35 , 52 , 53 , 54 ]. In this study, the fungal community of the ghost moth populations primarily consisted of Ascomycota and Basidiomycota , which was similar to other lepidopteran insects [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Gut Bacterial and Fungal Communities of the Wild and Laboratory-Reared Thitarodes Larvae, Host of the Chinese Medicinal Fungus Ophiocordyceps sinensis on Tibetan Plateau

Liu

Zheng

Long

et al. 2021

Insects

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By employing a culture-dependent and -independent 16S rRNA and ITS gene high-throughput sequencing analyses, comprehensive information was obtained on the gut bacterial and fungal communities in the ghost moth larvae of three different geographic locations from high-altitude on Tibet plateau and from low-altitude laboratory. Twenty-six culturable bacterial species belonging to 21 genera and 14 fungal species belonging to 12 genera were identified from six populations by culture-dependent method. Carnobacterium maltaromaticum was the most abundant bacterial species from both the wild and laboratory-reared larvae. The most abundant OTUs in the wild ghost moth populations were Carnobacteriaceae, Enterobacteriaceae for bacteria, and Ascomycota and Basidiomycota for fungi. Larval microbial communities of the wild ghost moth from different geographic locations were not significantly different from each other but significant difference in larval microbial community was detected between the wild and laboratory-reared ghost moth. The larval gut of the wild ghost moth was dominated by the culturable Carnobacterium. However, that of the laboratory-reared ghost moth exhibited significantly abundant Wolbachia, Rhizobium, Serratia, Pseudomonas, and Flavobacterium. Furthermore, the larval gut of the wild ghost moth had a significantly higher abundance of Ophiocordyceps but lower abundance of Candida and Aspergillus than that of the laboratory-reared ghost moth.

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“…These results suggest that amplicon sequencing targeting the 16S rRNA V3–V4 regions, which is commonly used to examine the diversity of bacteria in a particular niche, may assign the genus Acetobacter to bacteria taxonomically closer to G55GP T . The genus Acetobacter is often identified by V3–V4 amplicon sequencing in insect guts, including beetles [41], fruit flies [42] and moths [43]. Although some fruit flies do actually harbour Acetobacter [44], it may well be that other insects carry bacteria more closely related to G55GP T .…”

Section: Genome Featuresmentioning

confidence: 99%

Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa

Guzman

Sombolestani

Poehlein

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterium designated G55GPT and pertaining to the family Acetobacteraceae was isolated from the gut of the Madagascar hissing cockroach Gromphadorhina portentosa. The Gram-negative cells were rod-shaped and non-motile. The complete 16S rRNA sequence of the strain G55GPT showed the highest pairwise similarity to Gluconacetobacter johannae CFN-Cf-55T (95.35 %), suggesting it represents a potential new genus of the family Acetobacteraceae . Phylogenetic analysis based on 16S rRNA gene and 106 orthologous housekeeping protein sequences revealed that G55GPT forms a monophyletic clade with the genus Commensalibacter , which thus far has also been isolated exclusively from insects. The G55GPT genome size was 2.70 Mbp, and the G+C content was 45.4 mol%, which is lower than most acetic acid bacteria (51–68 mol%) but comparable to Swingsia samuiensis AH83T (45.1 mol%) and higher than Commensalibacter intestini A911T (36.8 mol%). Overall genome relatedness indices based on gene and protein sequences strongly supported the assignment of G55GPT to a new genus within the family Acetobacteraceae . The percentage of conserved proteins, which is a useful metric for genus differentiation, was below 54 % when comparing G55GPT to type strains of acetic acid bacteria, thus strongly supporting our hypothesis that G55GPT is a member of a yet-undescribed genus. The fatty acid composition of G55GPT differed from that of closely related acetic acid bacteria, particularly given the presence of C19 : 1 ω9c/ω11c and the absence of C14 : 0 and C14 : 0 2-OH fatty acids. Strain G55GPT also differed in terms of metabolic features such as its ability to produce acid from d-mannitol, and its inability to produce acetic acid from ethanol or to oxidize glycerol to dihydroxyacetone. Based on the results of combined genomic, phenotypic and phylogenetic characterizations, isolate G55GPT (=LMG 31394T=DSM 111244T) is considered to represent a new species in a new genus, for which we propose the name Entomobacter blattae gen. nov., sp. nov.

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Similar Gut Bacterial Microbiota in Two Fruit-Feeding Moth Pests Collected from Different Host Species and Locations

Cited by 12 publications

References 68 publications

Comparison of Gut Bacterial Communities of Grapholita molesta (Lepidoptera: Tortricidae) Reared on Different Host Plants

Comparison of Gut Bacterial Communities of Grapholita molesta (Lepidoptera: Tortricidae) Reared on Different Host Plants

Gut Bacterial and Fungal Communities of the Wild and Laboratory-Reared Thitarodes Larvae, Host of the Chinese Medicinal Fungus Ophiocordyceps sinensis on Tibetan Plateau

Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa

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