Similar to oleic acid, eicosapentaenoic acid stimulates apolipoprotein B secretion by inhibiting its intracellular degradation in Hep G2 cells

Kurokawa, Munekazu; Hirano, Tsutomu; Furukawa, Seiichi; Nagano, Seishi; Adachi, Mitsuru

doi:10.1016/0021-9150(94)05399-4

Cited by 10 publications

(5 citation statements)

References 24 publications

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“…In contrast apoAIV secretion reaches its maximum at 2 mM and does not increase thereafter. It may be hypothesized that this relative excess of apoAIV compared with lipids could lead to degradation of part of the protein molecules as it has been shown for other apolipoproteins [Fast et al, 1993; Kurokawa et al, 1995].…”

Section: Discussionmentioning

confidence: 99%

Butyrate stimulates ApoA‐IV‐containing lipoprotein secretion in differentiated Caco‐2 cells: Role in cholesterol efflux

Nazih

Nazih‐Sanderson

Krempf

et al. 2001

J of Cellular Biochemistry

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The aim of this study was to determine: (1) whether the Short Chain Fatty Acids (SCFA) Acetate, Propionate, and Butyrate enhance the synthesis and secretion of intestinal apolipoprotein A-IV-containing lipoproteins and (2) if so, whether these particles are able to promote cholesterol efflux in vitro. For this purpose Caco-2 cells were used for their functional properties of differentiated enterocytes. They were incubated with the three SCFA (2, 4, and 8 mM) for 48 h. Only butyrate stimulated apoA-IV gene expression and this was associated with an increase in apoA-IV secretion. A nondenaturing 2D-PAGE (agarose gel was followed by PAGE) was used to identify apoA-IV-containing lipoproteins in various media, and showed that butyrate stimulated the secretion of two small HDL sized particles. The influence of these secreted particles on cholesterol efflux was investigated using incubation of media with (3)H-cholesterol-labeled Fu5AH cells. The data indicate that conditioned media from Caco-2 cells treated with butyrate resulted in an increase of 20-30% in cholesterol efflux. We conclude that butyrate may regulate apoA-IV secretion and, therefore, modulate reverse cholesterol transport.

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Section: Discussionmentioning

confidence: 99%

Butyrate stimulates ApoA‐IV‐containing lipoprotein secretion in differentiated Caco‐2 cells: Role in cholesterol efflux

Nazih

Nazih‐Sanderson

Krempf

et al. 2001

J of Cellular Biochemistry

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“…Variation of sEH activity may modify plasma 15d-PGJ 2 levels and affect lipid metabolism through PPARc activation. Another type of EET, eicosapentaenoic acid, regulates triglyceride synthesis (Wang et al, 1993, Kurokawa et al, 1995, Rustan et al, 1988. Relationships between these epoxides and the lipoprotein phenotype of patients should be clarified in the future study.…”

Section: Discussionmentioning

confidence: 99%

Soluble epoxide hydrolase variant (Glu287Arg) modifies plasma total cholesterol and triglyceride phenotype in familial hypercholesterolemia: intrafamilial association study in an eight-generation hyperlipidemic kindred

et al. 2003

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Plasma lipid and lipoprotein in general reflect the complex influences of multiple genetic loci, for instance, even familial hypercholesterolemia (FH), a representative example of monogenic hyperlipidemia, often presents with phenotypic heterogeneity. In the course of investigating familial coronary artery disease in Utah, we studied 160 members of an eight-generation extended family of FH in which 69 members were affected with type IIa hyperlipoproteinemia (HLPIIa; high plasma cholesterol) and ten with type IIb hyperlipoproteinemia (HLPIIb; high plasma cholesterol as well as plasma triglyceride). Soluble epoxide hydrolase (EPHX2, sEH) plays a role in disposition of epoxides in plasma lipoprotein particles. Intrafamilial correlation analysis of the modifier effect of Glu287Arg substitution in the EPHX2 gene was carried out among 79 LDLR mutation carriers and 81 noncarriers. In the carriers, plasma cholesterol levels were elevated among carriers of the 287Arg allele (mean±SD=358 ± 72 mg/dl) in comparison with 287Glu homozygotes (mean±SD=302 ± 72 mg/dl) (p=0.0087). Similarly, in the LDLR mutation carriers, the plasma triglyceride levels were elevated among carriers of the 287Arg allele (mean ± SD=260 ± 100 mg/dl) in comparison with 287Glu homozygotes (mean ± SD= 169 ± 83 mg/dl) (p=0.020). No such gene-interactive effect was observed among noncarriers of the LDLR mutation. Half of the patients who presented with HLPIIb had inherited a defective LDLR allele as well as an EPHX2-287Arg allele, whereas the majority who presented with HLPIIa had a defective LDLR allele but not an EPHX2-287Arg allele. These results indicate a significant modification of the phenotype of FH with defective LDLR allele by EPHX2-287Arg variation in our studied kindred.

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“…Increased Apo B secretion can be the result of increased apo B synthesis, and/or decreased apo B removal through lowered LDL receptor activity. It has been reported that LA and EPA increased apo B secretion in HepG2 cells (Kurokawa et al. 1995; Arrol et al.…”

Section: Discussionmentioning

confidence: 98%

“…2000). It has been suggested that the increased apo B secretion by EPA is due to inhibition of apo B intracellular degradation (Kurokawa et al. 1995).…”

Section: Discussionmentioning

confidence: 99%

Influence of Stearidonic Acid on Lipoprotein Secretion and Fatty Acid Composition in Hepg2 Cells

Park

Albright

Storkson

et al. 2007

Journal of Food Lipids

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The health benefits of w-3 fatty acids, particularly fish oils, are well known. It is generally recommended to obtain w-3 fatty acids from fatty fish; however, the possible contaminants in fish products may deter this. Thus, supplements of w-3 fatty acids from plant-originated lipids may be of interest. Among these, a-linolenic acid (ALA) and stearidonic acid (SDA) have drawn attention. Thus, the purpose of this research was to test the possible conversion of these fatty acids, particularly SDA, to eicosapentaenoic acid (EPA) using a human hepatoma cell line, HepG2 cells. In addition, even though w-3 fatty acids have been shown to reduce hyperlipidemia, the effects on lipoprotein metabolism are relatively inconsistent. Thus, we also tested w-3 fatty acids along with SDA on apolipoprotein A and B (apo A and B) secretion from this cell line. The results obtained suggest that both ALA and SDA were converted to EPA and they increased apo B secretion, while having no effect on apo A secretion, similar to the effects of EPA and docosahexaenoic acid. Thus, it may be concluded that SDA can be a possible candidate for use as a precursor for EPA and its effect on lipoproteins was similar to that of EPA.3 Corresponding

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Similar to oleic acid, eicosapentaenoic acid stimulates apolipoprotein B secretion by inhibiting its intracellular degradation in Hep G2 cells

Cited by 10 publications

References 24 publications

Butyrate stimulates ApoA‐IV‐containing lipoprotein secretion in differentiated Caco‐2 cells: Role in cholesterol efflux

Butyrate stimulates ApoA‐IV‐containing lipoprotein secretion in differentiated Caco‐2 cells: Role in cholesterol efflux

Soluble epoxide hydrolase variant (Glu287Arg) modifies plasma total cholesterol and triglyceride phenotype in familial hypercholesterolemia: intrafamilial association study in an eight-generation hyperlipidemic kindred

Influence of Stearidonic Acid on Lipoprotein Secretion and Fatty Acid Composition in Hepg2 Cells

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