1998
DOI: 10.1093/nar/26.2.391
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Similarities and differences among 105 members of the Int family of site-specific recombinases

Abstract: Alignments of 105 site-specific recombinases belonging to the Int family of proteins identified extended areas of similarity and three types of structural differences. In addition to the previously recognized conservation of the tetrad R-H-R-Y, located in boxes I and II, several newly identified sequence patches include charged amino acids that are highly conserved and a specific pattern of buried residues contributing to the overall protein fold. With some notable exceptions, unconserved regions correspond to… Show more

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Cited by 426 publications
(454 citation statements)
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“…S7B). Notably, as in the case of the Cre and Flp recombinases, ACV ORF33 possesses an active-site tryptophan instead of the histidine residue found in the active sites of the vast majority of tyrosine recombinases (27,28). The closest homolog of ORF33 (with the same domain organization) is encoded in the genome of the ammonia-oxidizing soil thaumarchaeon Candidatus Nitrosopumilus koreensis, whereas weak hits to proteins from other organisms are confined to the catalytic domain.…”
Section: Resultsmentioning
confidence: 99%
“…S7B). Notably, as in the case of the Cre and Flp recombinases, ACV ORF33 possesses an active-site tryptophan instead of the histidine residue found in the active sites of the vast majority of tyrosine recombinases (27,28). The closest homolog of ORF33 (with the same domain organization) is encoded in the genome of the ammonia-oxidizing soil thaumarchaeon Candidatus Nitrosopumilus koreensis, whereas weak hits to proteins from other organisms are confined to the catalytic domain.…”
Section: Resultsmentioning
confidence: 99%
“…2,4 Replacement of E319 by any of several amino acids (including glycine), whose only common element is loss of a negative charge, increases recombination of HK022 sites. 22 These considerations suggest that E319 does not directly contact core site DNA, but instead affects site recognition indirectly, perhaps through interaction with residues that themselves contact DNA.…”
Section: Discussionmentioning
confidence: 99%
“…Family members are characterized by an invariant tyrosine residue that becomes transiently joined to the DNA backbone during strand cleavage and by several other highly conserved amino acids that activate strand cleavage, exchange, and rejoining. 2 Comparison of the three-dimensional structures of four widely diverged family members reveals considerable conservation of structure around the catalytic center. 3 -6 Phage l Int (Int-l) recognizes two distinct sequence motifs, the arm-type and the core-type binding sites (Figure 1).…”
Section: Introductionmentioning
confidence: 99%
“…1 A). This integrase belongs to the tyrosine recombinase family that catalyzes a sitespecific recombination between a chromosomal site (attB) and a similar or identical sequence (attP) found on mobile genetic elements including bacteriophages, insertion sequences, and transposons (15,16). To explore the biological functions of PAPI-1 int, we generated an int deletion mutant of strain PA14 (PA14⌬int) and examined the excision of PAPI-1 in this mutant strain.…”
mentioning
confidence: 99%