Background: Running shoes with carbon plates have been identified to have positive effects on improving running performance from a biomechanical perspective. However, the specific difference between the effects of carbon plates with different longitudinal bending stiffness (LBS)on biomechanical characteristics and muscular mechanics of lower limbs in adolescent runners remains unclear. This study aimed to identify the difference in biomechanical characteristics and muscular mechanics in lower limbs during running stance phases between wearing shoes with low longitudinal bending stiffness (Llbs) and high longitudinal bending stiffness (Hlbs) carbon plates in adolescent runners.Methods: 10 male adolescent runners with a habit of daily running exercise (age: 13.5 ± 0.6 years; height: 166.3 ± 1.9 cm; bodyweight: 50.8 ± 3.1 kg; foot length: 25.4 ± 0.2 cm) were recruited and asked to conduct two times of tests by wearing shoes with Llbs and Hlbs carbon plates in a randomized order. Paired t-test and statistical parametric mapping (SPM) analysis were used to identify the difference in biomechanical characteristics and muscular mechanics in lower limbs during running stance phases.Result: Under the condition of wearing shoes with Hlbs, the time of foot contact significantly increased, whereas the range of motion (ROM) of hip and metatarsophalangeal (MTP) in the sagittal plane significantly reduced as well as the peak moment of ankle joint in the sagittal plane. The activations of vastus medialis, vastus lateralis, flexor digitorum brevis (flex dig brevis), and flexor hallucis longus (flex hall long) significantly increased under the condition of wearing shoes with Hlbs. According to the results of the SPM analysis, the joint angles (hip, ankle, and MTP), the net joint moments (knee, ankle, and MTP), and the muscle forces (gluteus maximus and tibialis anterior) were significant difference during the running stance phase between conditions of wearing shoes with Hlbs and Llbs.Conclusion: Running shoes with Llb carbon plates are appropriate for adolescent runners due to the advantages of biomechanical characteristics and muscular mechanics.