Similarities and distinctions between murine natural killer cells and lymphokine-activated killer cells

Merluzzzi, Vincent J.; Smith, Mark D.; Last-Barney, Kathleen

doi:10.1016/0008-8749(86)90054-7

Cited by 18 publications

(5 citation statements)

References 9 publications

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“…Previous studies have shown that IL-2 activation of lymphoid cultures results in a decrease in the number of IL-2-unresponsive lymphocytes, with a concomitant increase in the number of lymphocytes responsive to IL-2 (39). Studies have indicated that at least 3 days of IL-2 activation are required for the induction of anti-P815 cell activity, with 7 days required for maximal activity (23,24). The results of this study do not demonstrate an absolute requirement for the expansion of an IL-2-activated cell population which mediates the C. albicans growth inhibition.…”

Section: Discussioncontrasting

confidence: 44%

“…The IL-2-activated lymphocytes are clearly lymphokine activated, but whether they are a lymphokine-activated killer lymphocyte (LAK) population or a subset of the LAK population is unclear. Various studies have revealed substantial heterogeneity in LAK populations following activation with IL-2 (24,26,32). The lytic activity of various types of LAK and their apparent reactivities are highly dependent on their states of activation.…”

Section: Discussionmentioning

confidence: 99%

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Growth inhibition of Candida albicans by interleukin-2-activated splenocytes

Beno

Mathews

1992

Infect Immun

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Murine splenocytes, Percoll-enriched low-density lymphocytes, and interleukin-2 (IL-2)-activated lymphocytes were assessed for the capacity to limit the growth of the hyphal form of Candida albicans. No fungal-growth-inhibitory activity was exhibited for C. albicans by either splenocytes or Percoll-enriched lymphocytes. These cells were capable of cytotoxic activity for a natural killer cell-sensitive cell line. However, when cultured for several days with IL-2, splenocytes acquired the capacity to inhibit the growth of the fungus. The appearance of the antifungal activity coincided with the development of cytotoxic activity for the natural killer cell-insensitive cell line. Anti-C. albicans and antitumor activities of IL-2-activated lymphocytes were competitively and reciprocally inhibited by C. albicans and the natural killer cell-sensitive and-insensitive cell lines. The antifungal activity of the IL-2-activated lymphocytes was exhibited against a number of clinical

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Section: Discussioncontrasting

confidence: 44%

Section: Discussionmentioning

confidence: 99%

Growth inhibition of Candida albicans by interleukin-2-activated splenocytes

Beno

Mathews

1992

Infect Immun

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“…In addition, LAK-like lytic activity against P815 targets was not detected in these animals. This is in agreement with an earlier report that beige mice are deficient in LAK precursor cells [7]. These data suggest that the beige defect may extend to non-MHC-restricted CTL, as well as L A K and N K cells.…”

Section: In Vivo Induction Of Cytolytic Activity With Anti-cd3 Monoclsupporting

confidence: 90%

Amelioration of experimental lung metastasis in mice by therapy with anti-CD3 monoclonal antibodies

Staňková

Anderson

Roder

1989

Cancer Immunol Immunother

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Binding of CD3-specific antibodies to the TcR-CD3 complex results in T cell activation without the need for occupation of the T cell receptor (TcR) by its ligand. Murine T cells activated in this manner will kill a broad range of tumor targets but not normal lymphoblasts. We report here that non-specific cytolytic activity can be induced in vivo by a single i.p. injection of nonlytic 145-2C11 anti-CD3 monoclonal antibody. At least three populations of effector cells are activated in these mice. These are non-MHC (major histocompatibility complex) restricted cytotoxic T lymphocytes, activated natural killer cells, and lymphokine-activated killer cells. Anti-CD3 treatment is effective in significantly reducing the number of lung tumor nodules which form in mice inoculated with oncogenic ras-transfected syngeneic 10T1/2 fibroblasts. Anti-CD3-activated killer cells may, therefore, find a future role in cancer immunotherapy.

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“…The major serologic phenotype of the LAK cell is Thy 1 +, Lyt 1-, Lyt 2 +, and asialo GMI-, and the minor phenotype is asialo GM1 + from normal mouse spleen ceils [15]. On the contrary, beige mice which lack NK effector cells and IL-2-inducible NK cells also lacked the ability to generate LAK cells [14]. Andriole et al demonstrated that the LAK cell system appeared to differ from the NK cell on the basis of an analysis of several congenitally immunodeficient mice.…”

Section: Discussionmentioning

confidence: 99%

Induction of activated natural killer cells from murine spleen cells primed in vivo and subsequently challenged in vitro with the streptococcal preparation OK432

Yamaue¹,

Katsumi²,

Tabuse³

et al. 1987

Cancer Immunol Immunother

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The present study shows that natural killer cell-mediated cytotoxicity of BALB/c mouse spleen cells to syngeneic tumor cells was augmented by in vivo priming or in vitro stimulation with the streptococcal preparation OK432. The augmentation of spleen cell cytotoxicity to syngeneic tumor cells by in vivo priming alone with OK432 was lower than that obtained by in vitro stimulation alone with OK432. When the murine spleen cells primed in vivo with OK432 were rechallenged in vitro with OK432 at various intervals, the natural cytotoxicity was more strongly enhanced than that seen with in vitro stimulation alone. The cell surface phenotype of killer cells activated with OK432 was Thy 1+ and asialo GM1+, suggesting the activated natural killer cell. Next, mice were transplanted with syngeneic colon adenocarcinoma cells, and primed in vivo with OK432. These spleen cells were subsequently challenged in vitro with OK432. These spleen cells displayed a strong cytotoxic activity not only to the transplanted adenocarcinoma cells but also to other syngeneic tumor cells.

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Similarities and distinctions between murine natural killer cells and lymphokine-activated killer cells

Cited by 18 publications

References 9 publications

Growth inhibition of Candida albicans by interleukin-2-activated splenocytes

Growth inhibition of Candida albicans by interleukin-2-activated splenocytes

Amelioration of experimental lung metastasis in mice by therapy with anti-CD3 monoclonal antibodies

Induction of activated natural killer cells from murine spleen cells primed in vivo and subsequently challenged in vitro with the streptococcal preparation OK432

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