Similarity between the high density lipoprotein profile of fetal lung and of amniotic fluid

Gebhardt, D. O. E.; Beintema, A.

doi:10.1016/0009-8981(80)90038-8

Cited by 3 publications

(2 citation statements)

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“…One reason for this may be their occurrence in amniotic fluid at only a few percent of human adult plasma concentrations [1], and thus a need for very sensitive apolipoprotein assays. In their studies Guibaud et al [2] found ai-lipoproteins in amniotic fluid, later confirmed by Gebhardt et al [3] and Gebhardt and Beintema [4], who extended the investigations on this lipoprotein fraction and in addi tion identified apo A-I (3). By raising antibodies in rab bits against concentrated amniotic fluid proteins from different time points during gestation, Sutcliffe and Brock [5] also identified di-lipoproteins.…”

Section: Introductionmentioning

confidence: 51%

“…The reasons are not yet apparent. In fetal lung homogenates, an HDL-like particle containing A-I was described [4], suggesting an additional source of am niotic fluid apo A-I, probably increasing with fetal growth. The augmentation in apo A-I may parallel the increase of lecithin in late pregnancy [14], Interestingly, apoproteins of pulmonary surfactant were detected in amniotic fluid from week 30 to 32 of gestation, increas ing severalfold to a maximum at week 37.…”

Section: Discussionmentioning

confidence: 99%

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Apolipoproteins in Human Amniotic Fluid: Concentrations, Isoforms and Polymorphism

Steinmetz

Sefraoui²,

Scheffler³

et al. 1989

Gynecol Obstet Invest

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Apolipoproteins were determined in 50 human amniotic fluids obtained by amniocentesis during weeks 16 and 22 (n = 26) or 33 and 41 (n = 24) of gestation. Whereas apo A-I, A-II, A-IV, and E were identified at levels of 1 0.7, 0.8 and 1% of normal human adult plasma, respectively, apo B levels were only 0.04% of plasma concentration and apo C-III levels were below the detection limits of the assay. Amniotic fluid levels of apo A-II, A-IV and E did not differ between early and late stages of pregnancy, but levels of apo B decreased and apo A-I increased significantly in late pregnancy. Isofocusing showed apo A-I, A-II and A-IV in identical positions as compared to human adult plasma. Furthermore the known genetic polymorphism of apo A-IV was detectable. Individuals heterozygous for the variant form apo A-IV-2, showing the phenotype apo A-IV (2–1), had significantly higher levels of apo A-I and A-II as compared to the common phenotype apo A-IV (1–1). We conclude that human amniotic fluid contains the major plasma apolipoproteins at about 1% of plasma levels with the exception of apo B which shows a level at an order of magnitude less than high-density lipoprotein apoproteins in comparison to their plasma counterparts.

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