Multiple-injection strategy that has been applied widely in diesel engines usually features a short duration for each injection pulse. As a result, the shortened injection makes the needle opening and closing transients increasingly important for spray in an injection event. Owing to the needle movement, the spray development during the transient processes is complex and quite different from the spray at the quasi-steady state. However, so far modeling of the spray development during the transient processes is far from adequate. Particularly, a theoretical zero-dimensional (0-D) spray tip penetration model considering the needle opening and sac pressurization processes as well as ambient and injection conditions during the start-of-injection (SOI) transients is still absent. In this paper, considering the sac pressurization processes, the 0-D model of spray tip penetration during the SOI transients is derived. Then, the model is validated against the experimental spray data using a long-distance microscope together with an ultrahigh speed CMOS camera. The model and experimental results show that the spray tip penetration shows a t3/2 dependence at the initial stage of injection rather than the t dependence suggested by Hiroyasu’s model. Later, the spray tip penetration shows a t3/4 dependence owing to the spray breakup, and a t1/2 dependence with the completion of sac pressurization. The models and analysis are believed to provide new insights into the transient spray behaviors and valuable reference for engineers and researchers who are considering the model-based development of next-generation diesel engines.