Simple analysis of total mercury and methylmercury in seafood using heating vaporization atomic absorption spectrometry

Yoshimoto, Keisuke; Anh, Hoang Thi Van; Yamamoto, Atsushi; Koriyama, Chihaya; Ishibashi, Yutaka; Tabata, Masaaki; Nakano, Atsuhiro; Yamamoto, Megumi

doi:10.2131/jts.41.489

Cited by 20 publications

(14 citation statements)

References 26 publications

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“…Similarly, Hg concentrations in sardines were lower than the Hg levels in canned sardines (117 ng/g, 141 ng/g, and 143 ng/g) reported by Boadi et al (2011). The current study found that the percentage of MeHg in T-Hg in canned fish samples ranged from 90.4% to 100%, which was consistent with levels found in fish, squid and shrimp in previous studies (Bloom, 1992;Yoshimoto et al, 2016;Hoang et al, 2017).…”

Section: Discussionsupporting

confidence: 88%

“…Extraction of T-Hg and MeHg from canned fish samples followed the protocol described previously (Miyamoto et al, 2010;Yoshimoto et al, 2016). Briefly, the muscle of canned fish samples was solubilized with 0.1% Cys solution (1 mL) and 5 M NaOH (1 mL), then incubated at 80°C for 1 hr with vortexing every 10 min.…”

Section: Determination Of Total Mercury and Methylmercury In Canned Fishmentioning

confidence: 99%

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Hair mercury levels in relation to fish consumption among Vietnamese in Hanoi

Hoang

Do²,

Agusa

et al. 2017

J. Toxicol. Sci.

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-People are exposed to methylmercury (MeHg) mainly through fish consumption, which is increasing in Vietnam. However, little information is available on estimating the health risk of MeHg exposure through fish consumption in Vietnam. The present study examined the association between mercury (Hg) levels in hair and selenium (Se) levels in toenails of 196 Vietnamese people and their fish consumption, using a dietary questionnaire to obtain information pertinent for assessing health risk owing to MeHg exposure. The geometric mean of Hg levels in the hair of males and females was 617 ng/g and 575 ng/g, respectively. We found that Hg levels in the hair of 98% of the Vietnamese study subjects were lower than the provisional tolerable weekly intake for MeHg (1.6 μg Hg/kg body weight; which is equivalent to a hair Hg concentration of approximately 2,300 ng/g, with an uncertainty factor of 6.4). There were significant differences in the age-adjusted geometric mean of Hg levels found in hair from females related to their frequency of freshwater fish consumption. The levels of Hg in hair and Se in toenails increased with an increased frequency of marine fish consumption, and both showed a significant positive correlation in subjects who consumed marine fish ≥ once/week. This is the first cross-sectional study to investigate the association between hair Hg levels and fish consumption in Vietnam. These findings provide valuable information for future assessments of the health risk of MeHg exposure through fish consumption in Vietnam.

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Section: Discussionsupporting

confidence: 88%

Section: Determination Of Total Mercury and Methylmercury In Canned Fishmentioning

confidence: 99%

Hair mercury levels in relation to fish consumption among Vietnamese in Hanoi

Hoang

Do²,

Agusa

et al. 2017

J. Toxicol. Sci.

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“…THg and MeHg extractions from earthworms were performed based on the reported protocol for biological tissue (Miyamoto et al, 2010;Yoshimoto et al, 2016). For THg extraction, an aliquot of approximately 0.1 g (dw) of each earthworm sample was weighed in 15 mL polypropylene (PP) tubes with screw caps, in triplicate.…”

Section: Extraction Of Mercury and Methylmercurymentioning

confidence: 99%

“…CRM165 (5.340 ± 0.140 mg kg -1 ) showed a THg recovery of 97.6 ± 4.3% (i.e., 5.210 ± 0.170 mg kg -1 ) with a CV of 4.4%, and CRM250 (i.e., 0.610 ± 0.020 mg kg -1 ) showed a recovery of 98.01 ± 2.9% (i.e., 0.580 ± 0.020 mg kg -1 ) with a CV of 2.95%. Therefore, we used 97.5% (the mean CRM MeHg recovery for swordfish and codfish) as the correction factor for calculating the concentration of MeHg extracted from earthworm samples (Yoshimoto et al, 2016;Miyamoto et al, 2010). The recovery tests for soil THg were measured using CRM lake sediments 7303-a (CRM E-0190) and marine sediments 7302-a (CRM A-0210).…”

Section: Mercury Determination Quality Control (Qc) and Quality Assmentioning

confidence: 99%

Comparison of mercury and methylmercury bioaccumulation in earthworms (<i>Bimastus parvus</i>) native to landfill-leachate-contaminated forest soil

Arizono

Ji³

et al. 2018

J. Toxicol. Sci.

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Total mercury (THg) and methylmercury (MeHg) bioaccumulation was explored in the Bimastus parvus species of earthworm (B. parvus) native to the leachate-contaminated forest soils around a Hg-polluted traditional landfill in Japan. General soil properties and concentrations of THg and MeHg in forest soils and in B. parvus were determined. The results indicated that the average THg concentrations in B. parvus and in forest soils in the leachate-contaminated sites were 10.21 and 14.90 times higher than those in the reference sites, respectively, whereas similar average MeHg concentrations were observed in forest soils (< 0.01 mg kg) and in B. parvus (0.100-0.114 mg kg) across all sampled sites. The average bioaccumulation factors of THg in B. parvus (BAF) in forest soil were similar between the leachate-contaminated sites and the reference sites. Cluster and regression analyses demonstrated that the B. parvus Hg (THg and MeHg) and soil THg were positively correlated with each other and with soil organic matter (SOM) and clays, but were negatively correlated with sand and hardly correlated with silts and pH in leachate-contaminated forest soils. From these results, it was proposed that Hg exposure to food chains is possible through B. parvus, because B. parvus showed a high ability to accumulate THg and MeHg in both leachate-contaminated and reference forest soils. Together, these findings indicated that the role of B. parvus in MeHg production is not clear, and it is possible that the MeHg in B. parvus was firstly formed within forest soils and then accumulated in their tissues.

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“…T‐Hg concentrations in blood and sciatic nerve were measured with a direct thermal decomposition Hg analyzer (SP‐3D and MA‐3000; Nippon Instruments, Tokyo, Japan) as previously described (Yamamoto et al, ; Yamamoto et al, ). T‐Hg and MeHg contents in brain tissue were measured using a protocol we developed previously (Hoang, Do, et al, ; Hoang, Sakamoto, & Yamamoto, ; Miyamoto, Kuwana, Ando, Yamamoto, & Nakano, ; Yoshimoto et al, ). The samples were solubilized with 0.1% Cys solution (1 mL) and 5 m NaOH (1 mL), then incubated at 80°C for 1 hour with vortexing every 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of neurobehavioral impairment in methylmercury‐treated KK‐Ay mice by dynamic weight‐bearing test

Yamamoto

Motomura

Yanagisawa

et al. 2018

J of Applied Toxicology

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Methylmercury (MeHg) is known to cause neurobehavioral impairment in human and experimental animals. We previously reported that MeHg (5 mg Hg/kg) induced severe neurobehavioral dysfunction in 4-week-old KK-Ay mice, although it is difficult to evaluate quantitatively the neurobehavioral impairment in MeHg-treated KK-Ay mice because of their obesity. The aim of this study was to evaluate MeHg-induced neurobehavioral dysfunction in KK-Ay mice using the dynamic weight-bearing test, which analyzes the animal's weight distribution between the four limbs. Male 12-week-old KK-Ay mice were treated with MeHg (5 mg Hg/kg) three times per week for 5 weeks. Body weight loss began after approximately 2 weeks of MeHg treatment, and decreased significantly at 4 weeks. Seven of the nine MeHg-treated mice exhibited overt neurological symptoms such as ataxia and gait disturbance. The weight-bearing load was lower for the forelimb than for the hindlimb at baseline and until 1 week after MeHg treatment was initiated. In weeks 2-4, the dynamic weight-bearing loads on the forelimb and hindlimb were similar. The load on the forelimb exceeded the load on the hindlimb after 5 weeks of treatment. This finding indicates that the dynamic weight-bearing test is useful for semi-quantitative evaluation of neurobehavioral impairment in MeHg-treated rodents, and is less stressful for the animals. Infiltration of CD204-positive macrophages was observed in the sciatic nerve of MeHg-treated mice, suggesting that CD204 can serve as a useful marker of tissue injury in peripheral nerves and a possible target in regenerating peripheral nerves and controlling neuropathies.

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Simple analysis of total mercury and methylmercury in seafood using heating vaporization atomic absorption spectrometry

Cited by 20 publications

References 26 publications

Hair mercury levels in relation to fish consumption among Vietnamese in Hanoi

Hair mercury levels in relation to fish consumption among Vietnamese in Hanoi

Comparison of mercury and methylmercury bioaccumulation in earthworms (<i>Bimastus parvus</i>) native to landfill-leachate-contaminated forest soil

Evaluation of neurobehavioral impairment in methylmercury‐treated KK‐Ay mice by dynamic weight‐bearing test

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