Simple and practical sandwich-type enzyme immunoassay for human oxidatively modified low density lipoprotein using antioxidized phosphatidylcholine monoclonal antibody and antihuman apolipoprotein-B antibody

Kohno, Hiroaki; Sueshige, Nobuyuki; Oguri, Kazuhito; Izumidate, Hironobu; Masunari, Toshiyuki; Kawamura, Mizuho; Itabe, Hiroyuki; Takano, Tatsuya; Hasegawa, Akira; Nagai, Ryozo

doi:10.1016/s0009-9120(00)00065-5

Cited by 69 publications

(46 citation statements)

References 32 publications

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“…Plasma Ox-LDL was assayed using a Kyowa Medex MX kit (Kyowa Medex Inc. Tokyo), which is a sandwich-type enzyme immunoassay using anti-oxidized phosphatidylcholine monoclonal antibody (DLH3) and anti-human apolipoprotein-B antibody. 15,16) Statistical analysis: Data are presented as the mean ± SD. The KolmogorovSmirnov test was used to determine whether or not the data were normally distributed.…”

Section: Methodsmentioning

confidence: 99%

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Tea Catechin Consumption Reduces Circulating Oxidized Low-Density Lipoprotein

et al. 2007

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SUMMARYIt has been reported that green tea consumption reduces the risk of coronary artery disease and cardiac events. Catechin is a major constituent of Japanese green tea and an antioxidant. Lipids and oxidization of low-density lipoprotein cholesterol (LDL-C) play important roles in atherosclerosis. Therefore, we evaluated the effect of catechin intake on the lipid profile and plasma oxidized LDL. The study population consisted of 40 healthy adult volunteers (10 men, 30 women). Catechin was extracted from green tea leaves. The subjects were randomly divided into two groups, a catechin group (n = 29) and a control group (n = 11). In the catechin group, catechin (500 mg: equivalent to 6 or 7 cups of green tea) was administered orally. Venous blood samples were obtained before eating a meal at the start and after 4 weeks without any lifestyle modification. Plasma oxidized LDL assay was performed with a sandwich-type enzyme immunoassay using anti-oxidized phosphatidylcholine monoclonal antibody. The baseline lipid profiles and tea consumptions were similar between the two groups. Plasma oxidized LDL was significantly decreased after catechin administration (from 9.56 ± 9.2 to 7.76 ± 7.7 U/mL, P = 0.005), while plasma LDL-C, triglyceride, and HDL-C concentrations did not change. Catechin decreased the plasma oxidized LDL concentration without significant change in plasma LDL concentration. The mechanism of the beneficial effects of green tea on coronary artery disease might result from a decrease in plasma oxidized LDL. (Int Heart J 2007; 48: 725-732)

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Section: Methodsmentioning

confidence: 99%

“…15,16) Two studies have reported the effects of statins on Ox-LDL. 17,18) Catechins are the major constituent of green tea and are antioxidants.…”

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confidence: 99%

Tea Catechin Consumption Reduces Circulating Oxidized Low-Density Lipoprotein

et al. 2007

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“…Measurement of plasma oxLDL concentration: Plasma oxLDL concentration was calculated by measurement of the apolipoprotein B-containing lipoproteins that were transporting oxidized phosphatidylcholine (PC); this was done with a sandwich enzyme-linked immunosorbent assay (Kyowa Medex, Tokyo, Japan) according to a method developed by Itabe et al 11,12 In this method, a mouse monoclonal antibody, FOH1a/ DLH3, is used as the first antibody. The second antibody is goat antihuman apolipoprotein B polyclonal antibody.…”

Section: Study Patientsmentioning

confidence: 99%

Oxidized low‐density lipoprotein and high‐density lipoprotein cholesterol modulate coronary arterial remodeling: An intravascular ultrasound study

Yoneyama

Arakawa

Yonemura

et al. 2003

Clinical Cardiology

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SummaryBackground: Oxidized low-density lipoprotein (oxLDL) not only plays an important role in plaque formation, but also impairs the endothelium-dependent relaxation. Constrictive remodeling rather than intimal hyperplasia mainly contributes to restenosis after balloon angioplasty. Probucol (powerful antioxidant) reduced restenosis rate by improving constrictive remodeling. Thus, oxLDL may modulate coronary arterial remodeling.Hypothesis: The study was designed for using intravascular ultrasound to test the hypothesis that arterial constrictive remodeling (CR) was associated with oxLDL in patients with coronary artery disease.Methods: Intravascular ultrasound was performed in 36 patients with de novo atherosclerotic coronary. Remodeling was defined and evaluated as follows: remodeling index (RI) = lesion vessel area (VA)/(proximal reference VA + distal reference VA)/2. Constrictive remodeling (CR) was defined as remodeling index (RI) < 0.9. Neutral and expansive remodeling (NER) was defined as RI ≥ 0.9. The level of plasma ox-LDL was measured by sandwich ELISA using the monoclonal antibody (DLH3)-recognized oxidatively modified lipoproteins and the antihuman apoprotein B monoclonal antibody.

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“…During diabetes, persistent hyperglycemia causes increased production of free radicals especially reactive oxygen species (ROS), for all tissues from glucose auto-oxidation and protein glycosylation. Free radicals are generated as by-products of normal cellular metabolism; however, several conditions are known to disturb the balance between ROS production and cellular defense mechanisms [5]. …”

Section: Discussionmentioning

confidence: 99%