Simple and rapid method determination for metformin in human plasma using high performance liquid chromatography tandem mass spectrometry: Application to pharmacokinetic studies

Simultaneous quantification of ramipril, glimepiride and metformin in human plasma by ultra-performance liquid chromatography – tandem mass spectrometry

“…Full details of recovery and matrix effect were discussed by (Matuszewski et al, 1998). Extraction recovery of the three studied drugs was assessed by comparing their peak areas in the pre-extraction samples, which are spiked plasma samples subjected to the whole extraction procedure, with their peak areas in the post extraction samples, which are spiked at the end of the extraction procedure.…”

“…GLM was determined by LC-MS/MS methods either individually or with some other drugs in biological samples (Salem et al, 2004;Chakradhar et al, 2007;Liu et al, 2010). LC-MS/MS was also used for quantification of MET either alone or in presence of other antidiabetic drugs (AbuRuz et al, 2005;Mistri et al, 2007;Zhang et al, 2007;Marques et al, 2007;Sengupta et al, 2009;Liu and Coleman, 2009). Ultra-performance liquid chromatography (UPLC) has a significant advantage in resoluting the separated anaytes, sensitivity and speed of analysis, especially when coupling with mass spectrometers.…”

Development of a rapid reversed phase-high performance liquid chromatography method for simultaneous determination of metformin and vildagliptin in formulation and human plasma

“…A sur vey of the literature revealed some highperformance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry methods for estimation of MET alone [9][10][11][12][13][14][15] and in combination with other drugs. Few analytical methods are documented in the literature for determination of VLD in solid dosage forms 16,17 or in plasma.…”

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

“…Literature survey revealed HPLC, RP-HPLC, LC-MS, Spectrofluorimetric and simultaneous UV spectrophotometric methods are reported for the estimation of metformin hydrochloride [2][3][4][5], pioglitazone hydrochloride [6][7][8][9] and glibenclamide [10] alone or in combination with other anti-diabetic agents. So the present study aim to develop a simple, selective and precise RP-HPLC method for the simultaneous estimation of MET, PIO and GLI in bulk drug samples and in combined dosage formulation.…”